Patents by Inventor Alrik Pieter Los

Alrik Pieter Los has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11447759
    Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.
    Type: Grant
    Filed: February 12, 2020
    Date of Patent: September 20, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
  • Patent number: 11339379
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Grant
    Filed: August 21, 2020
    Date of Patent: May 24, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Remko Tsjibbe Winter, Ben Den Dulk
  • Patent number: 11078504
    Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: August 3, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Zheng Zhao, René Verwaal, Jacobus Thomas Pronk, Rolf Poldermans
  • Publication number: 20210087544
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chose from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filam-tous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be use in a process for the saccharification of cellulosic material.
    Type: Application
    Filed: December 1, 2020
    Publication date: March 25, 2021
    Inventors: Alrik Pieter LOS, Cornelis Maria Jacobus SAGT, Margot Elisabeth Francoise SCHOONEVELD-BERGMANS, Robbertus Antonius DAMVELD
  • Patent number: 10920207
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chose from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filam-tous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be use in a process for the saccharification of cellulosic material.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: February 16, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schooneveld-Bergmans, Robbertus Antonius Damveld
  • Publication number: 20200385692
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Application
    Filed: August 21, 2020
    Publication date: December 10, 2020
    Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Remko Tsjibbe WINTER, Ben DEN DULK
  • Patent number: 10787649
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Grant
    Filed: July 11, 2017
    Date of Patent: September 29, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Remko Tsjibbe Winter, Ben Den Dulk
  • Publication number: 20200190495
    Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.
    Type: Application
    Filed: February 12, 2020
    Publication date: June 18, 2020
    Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Maaike APPELDOORN
  • Publication number: 20200181657
    Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Application
    Filed: December 22, 2016
    Publication date: June 11, 2020
    Inventors: Alrik Pieter LOS, Zheng ZHAO, René VERWAAL, Jacobus Thomas PRONK, Rolf POLDERMANS
  • Patent number: 10655115
    Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.
    Type: Grant
    Filed: January 17, 2019
    Date of Patent: May 19, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
  • Publication number: 20190225947
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Application
    Filed: July 11, 2017
    Publication date: July 25, 2019
    Applicant: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Remko Tsjibbe WINTER, Ben DEN DULK
  • Patent number: 10316305
    Abstract: The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: June 11, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
  • Publication number: 20190136213
    Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72.
    Type: Application
    Filed: January 17, 2019
    Publication date: May 9, 2019
    Inventors: Alrik Pieter LOS, Rene Marcel De Jong, Maaike Appeldoorn
  • Publication number: 20180298363
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chose from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filam-tous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be use in a process for the saccharification of cellulosic material.
    Type: Application
    Filed: June 22, 2018
    Publication date: October 18, 2018
    Inventors: Alrik Pieter Los, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schooneveld-Bergmans, Robbertus Antonius Damveld
  • Patent number: 10077435
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: September 18, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schooneveld-Bergmans, Robbertus Antonius Damveld
  • Publication number: 20180245059
    Abstract: The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
    Type: Application
    Filed: April 25, 2018
    Publication date: August 30, 2018
    Inventors: Alrik Pieter LOS, Rene Marcel DE JONG, Maaike APPELDOORN
  • Patent number: 10035999
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chose from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filam-tous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be use in a process for the saccharification of cellulosic material.
    Type: Grant
    Filed: November 16, 2017
    Date of Patent: July 31, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schooneveld-Bergmans, Robbertus Antonius Damveld
  • Patent number: 9988615
    Abstract: The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: June 5, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
  • Publication number: 20180066243
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chose from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filam-tous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be use in a process for the saccharification of cellulosic material.
    Type: Application
    Filed: November 16, 2017
    Publication date: March 8, 2018
    Inventors: Alrik Pieter LOS, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schooneveld-Bergmans, Robbertus Antonius Damveld
  • Patent number: 9738881
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.
    Type: Grant
    Filed: July 8, 2015
    Date of Patent: August 22, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schoonneveld-Bergmans, Robbertus Antonius Damveld