Abstract: A culture chamber for microbiological competition assays using volatile compounds is provided, having a first receptacle, a second receptacle and a central part between the first receptacle and the second receptacle, wherein the receptacles have an outer wall and a perimeter wall perpendicular to the outer wall and wherein the central part has an intermediate wall with at least one opening and two side walls that extend from the perimeter of the intermediate wall in opposite directions, such that the side walls of the central part surround the perimeter walls of the receptacles and the perimeter walls of the receptacles are supported on the intermediate wall.