Abstract: The present invention in a first aspect relates to a method for generating three-dimensional multicellular spheroids from at least one eukaryotic cell, comprising seeding at least one cell onto a scaffold comprising at least one bi-layer comprising a first layer and a second layer (106) with alternating charges, said first layer comprising a polyelectrolyte being biocompatible with said cells and said second layer comprising nanocellulose.

Abstract: A lysis buffer comprising saponin and cholate and a method for selectively lysing mammalian eukaryotic cells in a liquid sample comprising mammalian eukaryotic cells and possibly microorganisms using the lysis buffer. A visualization buffer comprising ferric cyanide and/or ferrocyanide and ferric citrate and a method for visualization of viable bacterial cells through formation of Prussian blue using the visualization buffer.

Abstract: A method, comprising the steps of providing a sample containing contaminating nucleoside diphosphates and/or nucleoside triphosphates, such as ATP and/or ATP analogues including deoxyribonucleoside triphosphates; reducing the amount of the contaminating nucleoside diphosphates and/or nucleoside triphosphates in the sample with an apyrase enzyme, wherein said apyrase enzyme is a Shigella flexneri apyrase; and performing an analysis of the sample, wherein said analysis comprises an assay that would have been affected by the contaminating nucleoside diphosphates and/or nucleoside triphosphates had they not been reduced in the reduction step.

Abstract: The invention provides a device and methods for separating and isolating cells.

Abstract: Leukocytes (e.g., neutrophils, monocytes and/or lymphocytes) can be captured and separated from blood by removing platelets using a spiral channel, followed by capturing individual leukocyte types in a series of cell capture channels having leukocyte binding moieties. Accordingly, various microfluidic-based cell affinity chromatography methods can be used to separate leukocytes from whole blood.