Patents by Inventor Amanda BORTOLINI SILVEIRA

Amanda BORTOLINI SILVEIRA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240229122
    Abstract: The present disclosure relates to a method for detecting a mutation in a microsatellite sequence locus of a target fragment from a DNA sample, comprising a step of subjecting said DNA sample to a digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers suitable for amplifying said target fragment of the DNA sample including said microsatellite sequence; a first MS oligonucleotide (MS) hydrolysis probe, labeled with a first fluorophore, wherein said first MS oligonucleotide probe is complementary to a wild-type sequence including the microsatellite sequence; a second oligonucleotide reference (REF) hydrolysis probe, labeled with a second fluorophore, wherein said second oligonucleotide REF probe is complementary to a wild-type sequence of said target DNA fragment which does not include said microsatellite sequence.
    Type: Application
    Filed: February 6, 2024
    Publication date: July 11, 2024
    Applicants: Institut Curie, Institut National de la Sante et de la Recherche Medicale, Universite de Versailles Saint-Quentin-en-Yvelines
    Inventors: Charlotte Proudhon, Amélie Kasperek, Amanda Bortolini Silveira, François-Clément Bidard, Marc-Henri Stern
  • Publication number: 20230117652
    Abstract: The invention relates to a method for detecting a mutation in a microsatellite sequence locus of a target fragment from a DNA sample, comprising subjecting said DNA sample to a digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers for amplifying said target fragment of the DNA sample including said microsatellite sequence; a first MS oligonucleotide (MS) hydrolysis probe, labeled with a first fluorophore, wherein said first MS oligonucleotide probe is complementary to a wild-type sequence including the microsatellite sequence; and a second oligonucleotide reference (REF) hydrolysis probe, labeled with a second fluorophore, wherein said second oligonucleotide REF probe is complementary to a wild-type sequence of said target DNA fragment which does not include said microsatellite sequence. The invention also encompasses methods for the diagnosis and prognosis of cancer and a method for determining the efficacy of a cancer treatment.
    Type: Application
    Filed: September 21, 2022
    Publication date: April 20, 2023
    Applicants: Institut Curie, Institut National de la Sante et de la Recherche Medicale, Universite de Versailles Saint-Quentin-en-Yvelines
    Inventors: Charlotte Proudhon, Amélie Kasperek, Amanda Bortolini Silveira, François-Clément Bidard, Marc-Henri Stern
  • Publication number: 20200291466
    Abstract: The invention relates to a method for detecting a mutation in a microsatellite sequence locus of a target fragment from a DNA sample, comprising subjecting said DNA sample to a digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers for amplifying said target fragment of the DNA sample including said microsatellite sequence; a first MS oligonucleotide (MS) hydrolysis probe, labeled with a first fluorophore, wherein said first MS oligonucleotide probe is complementary to a wild-type sequence including the microsatellite sequence; a second oligonucleotide reference (REF) hydrolysis probe, labeled with a second fluorophore, wherein said second oligonucleotide REF probe is complementary to a wild-type sequence of said target DNA fragment which does not include said microsatellite sequence. The invention also encompasses methods for the diagnosis and prognosis of cancer and a method for determining the efficacy of a cancer treatment.
    Type: Application
    Filed: July 11, 2018
    Publication date: September 17, 2020
    Applicants: Institut Curie, Institut National de la Santé et de la Recherche Médicale, Universite de Versailles Saint-Quentin-en-Yvelines
    Inventors: Charlotte PROUDHON, Amélie KASPEREK, Amanda BORTOLINI SILVEIRA, François-Clément BIDARD, Marc-Henri STERN