Patents by Inventor Amar Gupta

Amar Gupta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for performing multiplexed real-time PCR
    Patent number: 11345958
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: May 7, 2021
    Date of Patent: May 31, 2022
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for performing multiplexed real-time PCR
    Patent number: 11198903
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: October 7, 2019
    Date of Patent: December 14, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20210269857
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: May 7, 2021
    Publication date: September 2, 2021
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Photocleavable nucleotide reagents with high stability
    Patent number: 11078534
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: August 3, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Alexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
  • Methods for performing multiplexed real-time PCR
    Patent number: 11034997
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: June 15, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for performing multiplexed PCR
    Patent number: 11028433
    Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: June 8, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Reversibly protected nucleotide reagents with high thermal stability
    Patent number: 10793901
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: October 6, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • Generic method for the stabilization of specific RNA
    Patent number: 10689689
    Abstract: The present invention relates to methods and compositions for the stabilization of specific RNA molecules that can either be the target for detection or the control standard.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: June 23, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch
    Patent number: 10590469
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: March 17, 2020
    Assignee: Roche Molecular Svstems, Inc.
    Inventors: Amar Gupta, Igor Kozlov, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20200048682
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: October 7, 2019
    Publication date: February 13, 2020
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • PHOTOCLEAVABLE NUCLEOTIDE REAGENTS WITH HIGH STABILITY
    Publication number: 20190161797
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Application
    Filed: November 26, 2018
    Publication date: May 30, 2019
    Inventors: Alexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR IN A SELF-CONTAINED NUCLEIC ACID ANALYSIS POUCH
    Publication number: 20190024155
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.
    Type: Application
    Filed: September 25, 2018
    Publication date: January 24, 2019
    Inventors: Amar Gupta, Igor Kozlov, Randall Saiki, Alison Tsan
  • COMPOSITIONS AND METHODS FOR IMPROVING THE THERMAL STABILITY OF NUCLEIC ACID AMPLIFICATION REAGENTS
    Publication number: 20180363044
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises nucleoside polyphosphates having four or more phosphates. The present invention also provides for methods for using the nucleoside polyphosphates having four or more phosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Application
    Filed: June 13, 2018
    Publication date: December 20, 2018
    Inventor: Amar Gupta
  • Nucleic acid target identification by structure based probe cleavage
    Patent number: 10106842
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: October 23, 2018
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • REVERSIBLY PROTECTED NUCLEOTIDE REAGENTS WITH HIGH THERMAL STABILITY
    Publication number: 20180179585
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Application
    Filed: December 18, 2017
    Publication date: June 28, 2018
    Inventor: Amar Gupta
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20180073064
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: September 15, 2017
    Publication date: March 15, 2018
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED PCR
    Publication number: 20180073056
    Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: September 15, 2017
    Publication date: March 15, 2018
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for the enrichment of mutated nucleic acid from a mixture
    Patent number: 9873908
    Abstract: The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: January 23, 2018
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Amar Gupta, Nancy Schoenbrunner, Kevin Janssen
  • Nucleic acid target identification by structure based probe cleavage
    Patent number: 9828629
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: November 28, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • MULTIPLEXED NUCLEIC ACID TARGET IDENTIFICATION BY STRUCUTRE BASED PROBE CLEAVAGE
    Publication number: 20170204462
    Abstract: The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5?-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.
    Type: Application
    Filed: March 28, 2017
    Publication date: July 20, 2017
    Inventor: Amar Gupta