Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium.

Abstract: A device for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises a container (1) for collecting the sample and a closure (2). The container (1) comprises a first end and a second end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The reservoir portion (5) comprises at least one anchor element (4) to locally catch a polymerized fibrin pellet formed upon the addition of the sample into the container. The separation and concentration process is operated by trapping the target particles or molecules into the so-formed polymerized fibrin pellet that are captured on the anchor element (4).

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium.

Abstract: A device for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises a container (1) for collecting the sample and a closure (2). The container (1) comprises a first end and a second end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The reservoir portion (5) comprises at least one anchor element (4) to locally catch a polymerized fibrin pellet formed upon the addition of the sample into the container. The separation and concentration process is operated by trapping the target particles or molecules into the so-formed polymerized fibrin pellet that are captured on the anchor element (4).

Abstract: A device for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises a container (1) for collecting the sample and a closure (2). The container (1) comprises a first end and a second end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The reservoir portion (5) comprises at least one anchor element (4) to locally catch a polymerized fibrin pellet formed upon the addition of the sample into the container. The separation and concentration process is operated by trapping the target particles or molecules into the so-formed polymerized fibrin pellet that are captured on the anchor element (4).

Abstract: A method of mixing magnetic particles (3) in a reaction chamber (2) that is part of a microfluidic device and that contains the said particles in suspension, comprises the steps: (a) providing an electromagnetic means (1,1?,6,7) to generate magnetic field sequences having polarity and intensity that vary in time and a magnetic field gradient that covers the whole space of the said reaction chamber (2); (b) applying a first magnetic field sequence to separate or confine the particles (3) so the particles occupy a sub-volume in the volume of the reaction chamber (2); (c) injecting a defined volume of the said reagent in the reaction chamber; and (d) applying a second magnetic field sequence to leads the particles (3) to be homogenously distributed and dynamically moving over a substantial portion of the whole reaction chamber volume.

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium,

Abstract: The invention discloses a method for selecting cells depending on their level of displaying and preferably secreting a protein of interest from a population of heterogeneously expressing cells, comprising: (a) contacting said cells with magnetic beads coated with an affinity group to the said cells, (b) mixing the said magnetic beads with the cells to capture the cells displaying/secreting the protein of interest, (c) performing at least one washing step to remove the non-captured cells, and (d) recovering the cells to which that magnetic beads have bound.

Abstract: A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium.

Abstract: device for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises a container (1) for collecting the sample and a closure (2). The container (1) comprises a first end and a second end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The reservoir portion (5) comprises at least one anchor element (4) to locally catch a polymerized fibrin pellet formed upon the addition of the sample into the container. The separation and concentration process is operated by trapping the target particles or molecules into the so-formed polymerized fibrin pellet that are captured on the anchor element (4).

Abstract: A magnetic particle based separation and assaying method uses at least two sets of magnetic particles placed in solution within a container and characterized respectively by a coercive field e1 and e2, wherein e1 is greater than e2. The first magnetic particles with a larger coercive field e1 will be used as carrier to handle the second affinity magnetic particles having a lower coercive field e2. A magnetic particles handling method includes the step of applying an external magnetic field having a polarity and amplitude that varies with time to cause the said carrier magnetic particles to be in relative motion within the container driving thereby the affinity particles to form an homogenous suspension of particles in perpetual relative movement with the respect to the liquid.

Abstract: A magnetic particle based separation and assaying method uses at least two sets of magnetic particles placed in solution within a container and characterized respectively by a coercive field e1 and e2, wherein e1 is greater than e2. The first magnetic particles with a larger coercive field e1 will be used as carrier to handle the second affinity magnetic particles having a lower coercive field e2. A magnetic particles handling method includes the step of applying an external magnetic field having a polarity and amplitude that varies with time to cause the said carrier magnetic particles to be in relative motion within the container driving thereby the affinity particles to form an homogenous suspension of particles in perpetual relative movement with the respect to the liquid.

Abstract: A fluidic assay system assembly comprising: (a) A disposable fluidic cartridge (1) comprising at least one reaction chamber (3) connected to a network of fluidic channels (2) with at least one inlet channel and one outlet channel. The said inlet and outlet channels end at the down side of the fluidic cartridge with at least two connecting pores (4), (4?); (b) A disposable vessel (5) comprising a connection tube 22 immersed in a sample container (6) and ended at the cap of the vessel with an external connection pore (7) ; (c) A fluidic manifold (8) that is interdependent with the bulk system (12) comprising a fluidic network connected (9) to active fluidic parts (10), (11). The said channel network ends at the top side of the fluidic manifold with at least one connecting pore (13). Wherein the first and the second pores of the fluidic cartridge are interfaced by direct physical contact with the sample container and the manifold pores, respectively.

Abstract: A magnetic particle based separation and assaying method uses at least two sets of magnetic particles placed in solution within a container and characterized respectively by a coercive field e1 and e2, wherein e1 is greater than e2. The first magnetic particles with a larger coercive field e1 will be used as carrier to handle the second affinity magnetic particles having a lower coercive field e2. A magnetic particles handling method includes the step of applying an external magnetic field having a polarity and amplitude that varies with time to cause the said carrier magnetic particles to be in relative motion within the container driving thereby the affinity particles to form an homogenous suspension of particles in perpetual relative movement with the respect to the liquid.

Abstract: A fluidic assay system assembly comprising: (a) a disposable fluidic cartridge (1) comprising at least one reaction chamber (3) connected to a network of fluidic channels (2) with at least one inlet channel and one outlet channel. The said inlet and outlet channels end at the down side of the fluidic cartridge with at least two connecting pores (4), (4?); (b) a disposable vessel (5) comprising a connection tube (22) immersed in a sample container (6) and ended at the cap of the vessel with an external connection pore (7); (c) a fluidic manifold (8) that is interdependent with the bulk system (12) comprising a fluidic network connected (9) to active fluidic parts (10), (11). The said channel network ends at the top side of the fluidic manifold with at least one connecting pore (13). Wherein the first and the second pores of the fluidic cartridge are interfaced by direct physical contact with the sample container and the manifold pores, respectively.

Abstract: The invention relates to a method of handling and mixing magnetic particles within a reaction chamber that is part of a microfluidic device and that contains the said particles in suspension. More particularly, the invention concerns a method of handling magnetic particles in a way to improve the mixing of the particles with the surrounding liquids medium and where the liquid is carried by a fluid flow as part of an automated system. Further, the invention describes the use of the method for conducting biological and chemical assays.

Abstract: A method of mixing magnetic particles (3) in a reaction chamber (2) that is part of a microfluidic device and that contains the said particles in suspension, comprises the steps: (a) providing an electromagnetic means (1,1?,6,7) to generate magnetic field sequences having polarity and intensity that vary in time and a magnetic field gradient that covers the whole space of the said reaction chamber (2); (b) applying a first magnetic field sequence to separate or confine the particles (3) so the particles occupy a sub-volume in the volume of the reaction chamber (2); (c) injecting a defined volume of the said reagent in the reaction chamber; and (d) applying a second magnetic field sequence to leads the particles (3) to be homogenously distributed and dynamically moving over a substantial portion of the whole reaction chamber volume.

Abstract: A device for manipulating and mixing magnetic particles (3) in a surrounding liquid medium, comprises at least one couple of magnetic poles (1,1?) facing each other across a gap, the facing poles diverging from a narrow end of the gap to a large end of the gap, the poles (1,1?) forming part of an electromagnetic circuit and being arranged to provide a magnetic field gradient in the gap region; and a reaction chamber (2) that is a part of a fluidic network for containing the said magnetic particles in suspension and placed in the gap of the said electromagnets poles (1,1?). The reaction chamber (2) preferably has at least one part which has a diverging cavity, arranged co-divergently in the diverging gap between the poles.

Abstract: A magnetic particle based separation and assaying method uses at least two sets of magnetic particles placed in solution within a container and characterized respectively by a coercive field e1 and e2, wherein e1 is greater than e2. The first magnetic particles with a larger coercive field e1 will be used as carrier to handle the second affinity magnetic particles having a lower coercive field e2. A magnetic particles handling method includes the step of applying an external magnetic field having a polarity and amplitude that varies with time to cause the said carrier magnetic particles to be in relative motion within the container driving thereby the affinity particles to form an homogenous suspension of particles in perpetual relative movement with the respect to the liquid.

Abstract: A device for manipulating and mixing magnetic particles (3) in a surrounding liquid medium, comprises at least one couple of magnetic poles (1,1?) facing each other across a gap, the facing poles diverging from a narrow end of the gap to a large end of the gap, the poles (1,1?) forming part of an electromagnetic circuit and being arranged to provide a magnetic field gradient in the gap region; and a reaction chamber (2) that is a part of a fluidic network for containing the said magnetic particles in suspension and placed in the gap of the said electromagnets poles (1,1?). The reaction chamber (2) preferably has at least one part which has a diverging cavity, arranged co-divergently in the diverging gap between the poles.