Abstract: This disclosure provides for an improved method of stabilizing spray-dried bacterial extracts with a composition comprising a stabilizer such that the extracts can be used in cell-free protein synthesis. Also provided herein are formulations for stable, spray-dried bacterial extracts that have increased protein synthesis activity compared to spray-dried bacterial extracts that do not contain a stabilizer.

Abstract: Presented herein is a high-throughput (HTP) genomic engineering platform for improving the production of therapeutic proteins in Chinese hamster ovary (CHO) cells. The disclosed HTP genomic engineering platform is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. The platform utilizes a unique suite of HTP genetic engineering tools to explore the genomic landscape associated with therapeutic protein production pathways, in order to unravel the biological drivers and disentangle the uncharacterized genetic architecture responsible for optimizing therapeutic protein production in CHO cells.

Abstract: A computer-implemented system and method for identifying stale configurations of a software-defined network (SDN) component uses a configuration export file of the SDN component to generate configuration dependency strands for configurations of the SDN component. The configuration dependency strands can then be classified as being stale based on at least one of path length, reference count and a custom attribute to elements in a network infrastructure for the configuration dependency strands.

Abstract: Presented herein is a high-throughput (HTP) genomic engineering platform for improving the production of therapeutic proteins in Chinese hamster ovary (CHO) cells. The disclosed HTP genomic engineering platform is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. The platform utilizes a unique suite of HTP genetic engineering tools to explore the genomic landscape associated with therapeutic protein production pathways, in order to unravel the biological drivers and disentangle the uncharacterized genetic architecture responsible for optimizing therapeutic protein production in CHO cells.

Abstract: A computer-implemented system and method for generating a correlation directed acyclic graph (CDAG) of configurations of a software-defined network (SDN) component for migration from a source computing environment to a destination computing environment uses a configuration export file of the SDN component to draw the CDAG. Correlation keys defined in the configuration export file are used to draw graph nodes. Contents associated with the correlation keys and template entries are used to draw edges between at least some of the graph nodes. The CDAG with the graph nodes and the edges is then displayed.

Abstract: Presented herein is a high-throughput (HTP) genomic engineering platform for improving the production of therapeutic proteins in Chinese hamster ovary (CHO) cells. The disclosed HTP genomic engineering platform is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. The platform utilizes a unique suite of HTP genetic engineering tools to explore the genomic landscape associated with therapeutic protein production pathways, in order to unravel the biological drivers and disentangle the uncharacterized genetic architecture responsible for optimizing therapeutic protein production in CHO cells.

Abstract: A system and method for migrating configurations of a software-defined network (SDN) component from a source computing environment to a destination computing environment uses a configuration export file that includes a metadata section and a templates section. The metadata section includes correlation keys for dependencies found in fetched configurations of the SDN component that correspond to dependency keywords in a configurable dictionary. The templates section includes reframed configurations of the dependencies with at least one reference to the correlation keys in the metadata section. At least some correlation-key references within reframed configurations in the templates section of the configuration export file are substituted with correlation-key replacements for the destination computing environment, which are then applied to a target SDN component in the destination computing environment.

Abstract: A computer-implemented system and method for identifying stale configurations of a software-defined network (SDN) component uses a configuration export file of the SDN component to generate configuration dependency strands for configurations of the SDN component. The configuration dependency strands can then be classified as being stale based on at least one of path length, reference count and a custom attribute to elements in a network infrastructure for the configuration dependency strands.

Abstract: A system and method for migrating configurations of a software-defined network (SDN) component from a source computing environment to a destination computing environment uses a configuration export file that includes a metadata section and a templates section. The metadata section includes correlation keys for dependencies found in fetched configurations of the SDN component that correspond to dependency keywords in a configurable dictionary. The templates section includes reframed configurations of the dependencies with at least one reference to the correlation keys in the metadata section. At least some correlation-key references within reframed configurations in the templates section of the configuration export file are substituted with correlation-key replacements for the destination computing environment, which are then applied to a target SDN component in the destination computing environment.

Abstract: A computer-implemented system and method for generating a correlation directed acyclic graph (CDAG) of configurations of a software-defined network (SDN) component for migration from a source computing environment to a destination computing environment uses a configuration export file of the SDN component to draw the CDAG. Correlation keys defined in the configuration export file are used to draw graph nodes. Contents associated with the correlation keys and template entries are used to draw edges between at least some of the graph nodes. The CDAG with the graph nodes and the edges is then displayed.

Abstract: Diagnostic systems and methods for a surge valve that selectively bypasses a compressor of a turbocharger of an engine involve receiving, by a controller, a barometric pressure signal indicative of a barometric pressure external to the engine, receiving, by the controller, a throttle inlet pressure (TIP) signal indicative of an air pressure at an inlet of a throttle valve of the engine and downstream from the surge valve and the compressor, and performing, by the controller, a diagnostic technique on the surge valve, the diagnostic technique comprising generating a pressure ratio signal that is a ratio of the TIP signal to the barometric pressure signal, applying a high-pass filter to the pressure ratio signal to obtain a filtered pressure ratio signal, and based on a quantity of detected pulses in the filtered pressure ratio signal that exceed the diagnostic threshold, detecting a malfunction of the surge valve.

Abstract: A system and method for visualizing and updating a data analytics model using Graphical User Interface (GUI) is disclosed. The system is configured to receive an input file, from a user, storing a data analytics model. Based upon the input file, the system identifies the standard template, from a set of template, corresponding to a data analytics module of the input file. Further, the system is configured to display a graphical representation corresponding to the data analytics model based upon the standard template of the data analytics model, wherein the graphical representation comprises a set of elements corresponding to the data analytics model. Further, the system enables editing one or more elements from the set of elements of the graphical representation. Upon modification of the elements of graphical representation, the system enables updating the input file, thereby update the data analytics model using the GUI.

Abstract: Diagnostic systems and methods for a surge valve that selectively bypasses a compressor of a turbocharger of an engine involve receiving, by a controller, a barometric pressure signal indicative of a barometric pressure external to the engine, receiving, by the controller, a throttle inlet pressure (TIP) signal indicative of an air pressure at an inlet of a throttle valve of the engine and downstream from the surge valve and the compressor, and performing, by the controller, a diagnostic technique on the surge valve, the diagnostic technique comprising generating a pressure ratio signal that is a ratio of the TIP signal to the barometric pressure signal, applying a high-pass filter to the pressure ratio signal to obtain a filtered pressure ratio signal, and based on a quantity of detected pulses in the filtered pressure ratio signal that exceed the diagnostic threshold, detecting a malfunction of the surge valve.

Abstract: A network management server allocates network addresses to a network gateway connected to a public network after receiving an allocation request that specifies a number of network addresses to allocate to the network gateway. The network management server determines a first and second subnets of the public network having, respectively, first and second pools of available network addresses capable of being allocated to devices on the public network, and allocates one or more available network addresses from the first and second pools to the network gateway.

Abstract: Multiple tenants within a hybrid cloud computing system may need IP addresses to communicate over a computer network external to the hybrid cloud system (such as the Internet). IP addresses are a scarce resource, and each address can only be assigned to a single tenant. With multiple tenants competing for IP addresses, many request collisions may occur if tenants request IP addresses in a naïve manner, such as by requesting the next available IP address numerically. When a collision occurs, a tenant must request a different IP address. Instead, tenants request random IP addresses within a particular subnet in a random manner, thereby reducing the number of collisions that occur and improving the latency associated with requesting an IP address.

Abstract: Metabolically engineered microorganism strains are disclosed, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product. Such chemical products include polyketides, 3-hydroxypropionic acid, and various other chemical products described herein. Methods of production also may be applied to further downstream products, such as consumer products. In various embodiments, modifications to a microorganism and/or culture system divert, at least transiently, usage of malonyl-coA from the fatty acid biosynthesis pathway and thereby provides for usage of the malonyl-coA for a chemical product other than a fatty acid. In various embodiments, the fatty acid biosynthesis pathway is modulated to produce specific fatty acids or combinations of fatty acids.

Abstract: Metabolically engineered microorganism strains are disclosed, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product. Such chemical products include polyketides, 3-hydroxypropionic acid, and various other chemical products described herein. Methods of production also may be applied to further downstream products, such as consumer products. In various embodiments, modifications to a microorganism and/or culture system divert, at least transiently, usage of malonyl-coA from the fatty acid biosynthesis pathway and thereby provides for usage of the malonyl-coA for a chemical product other than a fatty acid. In various embodiments, the fatty acid biosynthesis pathway is modulated to produce specific fatty acids or combinations of fatty acids.

Abstract: A new class of antibodies having specificity for glycoproteins are described. The antibodies are shown to bind sensitively and specifically to mannosylated proteins, such as proteins produced by fungi. Assays using these anti-glycoprotein antibodies for monitoring the presence of glycoproteins in a sample are provided. Such methods can be used to monitor methods for production and/or purification of desired polypeptides, which may be used to modify process parameters to modify (e.g., decrease or increase) the amount of glycosylated polypeptide produced and/or present in the purified product. Also provided are methods of using the subject antibodies for detecting the level of expression and secretion of a polypeptide, and methods of using the subject antibodies to purify or deplete a glycoprotein from a sample. In exemplary embodiments, the desired polypeptide may be a multi-subunit protein, such as an antibody, which may be produced in a yeast, such as Pichia pastoris.

Abstract: A system and method for visualizing and updating a data analytics model using Graphical User Interface (GUI) is disclosed. The system is configured to receive an input file, from a user, storing a data analytics model. Based upon the input file, the system identifies the standard template, from a set of template, corresponding to a data analytics module of the input file. Further, the system is configured to display a graphical representation corresponding to the data analytics model based upon the standard template of the data analytics model, wherein the graphical representation comprises a set of elements corresponding to the data analytics model. Further, the system enables editing one or more elements from the set of elements of the graphical representation. Upon modification of the elements of graphical representation, the system enables updating the input file, thereby update the data analytics model using the GUI.

Abstract: Multiple tenants within a hybrid cloud computing system may need IP addresses to communicate over a computer network external to the hybrid cloud system (such as the Internet). IP addresses are a scarce resource, and each address can only be assigned to a single tenant. With multiple tenants competing for IP addresses, many request collisions may occur if tenants request IP addresses in a naive manner, such as by requesting the next available IP address numerically. When a collision occurs, a tenant must request a different IP address. Instead, tenants request random IP addresses within a particular subnet in a random manner, thereby reducing the number of collisions that occur and improving the latency associated with requesting an IP address.