Abstract: The present invention discloses a device and method for multidimensional cell culture, a more particularly three-dimension (3D) and four-dimension (4D) device and method. The device and method of the present invention comprises growing cells as spheroids/tissueoids on non-woven fabric scaffold to create 3D tissue-like structures. The fourth dimension is provided by ability of the system to generate the 3D tissueoids in a much less time span and their ability to grow for extended period of time, even for greater than one year. The present invention also provides methods of use for analysis of cell-drug sensitivity of the device. Further, the invention provides a device for growth and drug sensitivity characterization of cells.