Abstract: Provided is a new agent for increasing the melting temperature (Tm value) of a nucleic acid. The present invention uses a specific (di)allylamine-based compound that meets a specific relationship between the molecular weight of the (di)allylamine-based compound and a numerical value calculated from a specific parameter related to the chemical structure of the (di)allylamine-based compound.

Abstract: An object of the present invention is to provide a convenient method capable of detecting a mutant gene at a mutant gene content rate that is lower than the detection limit of the mutant gene according to a clamp-PCR method; and a kit that implements the same. It is another object of the present invention to provide a convenient method capable of selectively inhibiting amplification of a target base sequence in a nucleic acid amplification reaction; and a kit for implementing the same. Inhibitory effect on the amplification of a nucleic acid having a base sequence complementary to a clamp nucleic acid in a nucleic acid amplification reaction by the clamp nucleic acid is enhanced in the coexistence of an amphoteric copolymer comprising a cationic structural unit (1) with an amino acid and an anionic structural unit (2), the mole ratio of the cationic structural unit (1) to the anionic structural unit (2) (cationic structural unit (1)/anionic structural unit (2)) being in the range of 0.13 to 1.

Abstract: One purpose of the present invention is to provide a simple method by which a mutant gene can be detected at a lower mutant gene content than the mutant gene detection limit by the ordinary clamp-PCR method, and a kit for performing the method. Another purpose of the present invention is to provide a simple method by which, in a nucleic acid amplification reaction, the amplification of a target base sequence can be selectively inhibited, and a kit for performing the method.

Abstract: In order to address problems in the prior art, the present invention provides a novel agent for raising the melting temperature (Tm) of a double-stranded nucleic acid, methods for detecting mismatching of a double-stranded nucleic acid or mutation with respect to a standard nucleic acid in a sample nucleic acid, and kits for carrying out these methods.

Abstract: It is an object of the present invention to provide a method for preparing or separating pancreatic progenitor cells (or a group of pancreatic progenitor cells) that do not contain undifferentiated cells and efficiently differentiate into pancreatic islet cells. More specifically, the present invention relates to: pancreatic progenitor cells that appear in the process of differentiation of stem cells into pancreatic islet cells, which are characterized in that the pancreatic progenitor cells are positive to CD82; an agent for treating blood glucose level impairment, wherein the agent comprises the pancreatic progenitor cells; and a method for preparing pancreatic progenitor cells, wherein the method comprises separating CD82-positive cells.