Patents by Inventor Amit Meller

Amit Meller has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11364499
    Abstract: Devices for detecting a molecule of interest comprising an electrokinetic focusing apparatus and a nanopore apparatus are provided. Kits and systems comprising the apparatus are also provided; as are methods of detecting molecules of interest comprising running the molecules through the electrokinetic focusing apparatus and then detecting the focused molecules as they pass through the nanopore.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: June 21, 2022
    Assignee: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED
    Inventors: Amit Meller, Moran Bercovici, Xander Frank Van Kooten, Joshua Spitzberg
  • Publication number: 20220036973
    Abstract: Methods for identifying a peptide by analyzing a linear readout representative of at least a portion of at least two amino acids along the peptide using a machine learning model, wherein the machine learning model is trained on linear readouts representative of a set of peptides of known sequence are provided. Methods of training a machine learning model on linear readouts representative of a set of known peptides, and systems for performing the methods of the invention are also provided.
    Type: Application
    Filed: October 24, 2019
    Publication date: February 3, 2022
    Inventors: Amit MELLER, Shilo OHAYON, Arik GIRSAULT
  • Publication number: 20220009034
    Abstract: Systems comprising a light source, thin membrane immersed in an aqueous solution and a system to direct and focus light from the light source to a spot on the membrane are provided. Methods of thinning and etching a membrane are also provided, as are membranes comprising a nanopore with a Gaussian curve shaped cross-section.
    Type: Application
    Filed: September 23, 2021
    Publication date: January 13, 2022
    Inventors: Amit MELLER, Tal GILBOA, Adam ZREHEN, Arik GIRSAULT, Eran ZVULONI
  • Publication number: 20210263011
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproducibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Application
    Filed: April 27, 2021
    Publication date: August 26, 2021
    Inventors: Amit MELLER, Meni WANUNU
  • Patent number: 11002724
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproductibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: May 11, 2021
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Amit Meller, Meni Wanunu
  • Publication number: 20210003547
    Abstract: Systems for detecting fluorescence from a molecule comprising an ion-impermeable film comprising at least one ion-conducting nanopore; a first and second liquid reservoir separated by the film; a means to induce movement of the molecule from the first reservoir to the second reservoir via the nanopore; a light source capable of exciting the molecule to emit fluorescence, wherein the light source shines into the second reservoir; a metallic layer adhered to the film by an adhesion layer and comprising a nanowell structure located adjacent to the nanopore; and a detector configured to detect the fluorescence emitted by the molecule are provided. Methods of use of the systems are also provided.
    Type: Application
    Filed: December 20, 2018
    Publication date: January 7, 2021
    Inventors: Amit MELLER, Ossama ASSAD, Tal GILBOA, Joshua SPITZBERG
  • Patent number: 10866230
    Abstract: Provided herein are compositions, compounds, processes, and methods of use of 3D porous coating(s) on or near a nanopore(s) for analysis or detection of charged polymers such as nucleic acids, proteins, protein-nucleic acid complexes, small molecule-biological complexes, polymer-biological complexes, and/or polyelectrolytes.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: December 15, 2020
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Mark Grinstaff, Amit Meller, Joseph Hersey, Allison Squires
  • Publication number: 20200340032
    Abstract: Methods and kits for detecting the presence of at least one target DNA sequence with or without a modification in a DNA molecule are provided.
    Type: Application
    Filed: July 31, 2018
    Publication date: October 29, 2020
    Inventors: Amit MELLER, Tal GILBOA, Chen TORFSTEIN, Yuval EBENSTEIN, Elmar WEINHOLD
  • Publication number: 20200216881
    Abstract: The present invention concerns compositions, kits and methods for genetic variation detection and classification. These methods combine the specificity of a ligation reaction with the single-molecule sensitivity of nanopore biosensors. This invention can achieve clinical detection of many diseases, including bacterial infections and cancer entities, by identification of specific genetic alterations.
    Type: Application
    Filed: June 14, 2018
    Publication date: July 9, 2020
    Inventors: Amit MELLER, Michelle PATKIN, Tal GILBOA, Yana BEN ZVI ROZEVSKY
  • Publication number: 20200171489
    Abstract: Devices for detecting a molecule of interest comprising an electrokinetic focusing apparatus and a nanopore apparatus are provided. Kits and systems comprising the apparatus are also provided; as are methods of detecting molecules of interest comprising running the molecules through the electrokinetic focusing apparatus and then detecting the focused molecules as they pass through the nanopore.
    Type: Application
    Filed: May 15, 2018
    Publication date: June 4, 2020
    Inventors: Amit MELLER, Moran BERCOVICI, Xander Frank VAN KOOTEN, Joshua SPITZBERG
  • Patent number: 10613076
    Abstract: Optoelectronic control of solid-state nanopores and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. Methods for controlling the translocation speed of biopolymers through solid-state nanopores and methods for unblocking clogged pores by illuminating nanopores are described.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 7, 2020
    Assignee: THE TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Amit Meller, Nicolas Di Fiori
  • Publication number: 20190025277
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproductibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Application
    Filed: September 21, 2018
    Publication date: January 24, 2019
    Inventors: Amit MELLER, Meni WANUNU
  • Patent number: 10101315
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproductibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: October 16, 2018
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Amit Meller, Meni Wanunu
  • Patent number: 9903820
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproducibly ceased, and (2) self-assembly under voltage driven electrolyte flow, in which 5 nm nanopore may be coated. Applications of chemically modified nanopore are provided including, the detection of biopolymers such as DNA and RNA, immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: February 27, 2018
    Assignee: The Trustees of Boston University
    Inventors: Amit Meller, Meni Wanunu
  • Publication number: 20170198346
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproductibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Application
    Filed: January 20, 2017
    Publication date: July 13, 2017
    Inventors: Amit MELLER, Meni WANUNU
  • Publication number: 20160169864
    Abstract: Provided herein are compositions, compounds, processes, and methods of use of 3D porous coating(s) on or near a nanopore(s) for analysis or detection of charged polymers such as nucleic acids, proteins, protein-nucleic acid complexes, small molecule-biological complexes, polymer-biological complexes, and/or polyelectrolytes.
    Type: Application
    Filed: May 30, 2014
    Publication date: June 16, 2016
    Inventors: Mark Grinstaff, Amit Meller, Joseph Hersey, Allison Squires
  • Publication number: 20160103067
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?19 nm diameter can be reproducibly ceased, and (2) self-assembly under voltage driven electrolyte flow, in which 5 nm nanopore may be coated. Applications of chemically modified nanopore are provided including, the detection of biopolymers such as DNA and RNA, immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Application
    Filed: July 31, 2015
    Publication date: April 14, 2016
    Inventors: Amit MELLER, Meni WANUNU
  • Publication number: 20160033471
    Abstract: Optoelectronic control of solid-state nanopores and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. Methods for controlling the translocation speed of biopolymers through solid-state nanopores and methods for unblocking clogged pores by illuminating nanopores are described.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 4, 2016
    Applicant: THE TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Amit MELLER, Nicolas Di FIORI
  • Patent number: 9121843
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproducibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Grant
    Filed: May 8, 2008
    Date of Patent: September 1, 2015
    Assignee: Trustees of Boston University
    Inventors: Amit Meller, Meni Wanunu
  • Patent number: 9046483
    Abstract: The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid.
    Type: Grant
    Filed: August 8, 2013
    Date of Patent: June 2, 2015
    Assignee: President and Fellows of Harvard College
    Inventors: Timothy J. Denison, Alexis Sauer-Budge, Jene A. Golovchenko, Amit Meller, Eric Brandin, Daniel Branton