Abstract: The present invention discloses a system and method for determination and prioritization of a plurality of secondary target protein. According to the invention, the present invention ensures the abstraction and authentication of possible scientific data for the primary target protein and introduces a statistical analysis of multiple similarity criteria to determine similar proteins in the form of the plurality of secondary target protein for an input query. Furthermore, the identified plurality of secondary target protein is analysed via matrix and multi-relational directed network analysis to prioritize and depict relationships among similarity concepts. Beneficially, the present invention ensures a high level of coverage and precision to descript protein-protein similarity.