Abstract: Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO: 44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.

Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.

Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.

Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.

Abstract: A system for editing of a target sequence at a locus of a host cell is disclosed. The system has a nucleic acid molecule comprising a nucleic acid segment comprising a targeting RNA sequence and an RNA segment that binds a protein. The system also has a nucleic acid molecule comprising a nucleic acid segment encoding a polypeptide with endonuclease activity fused to a protein that binds the RNA segment. The system also comprises a double stranded DNA molecule comprising DNA comprising at least one nucleotide sequence that is capable of binding to the target sequence at the locus.

Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).

Abstract: A system for editing of a target sequence at a locus of a host cell is disclosed. The system has a nucleic acid molecule comprising a nucleic acid segment comprising a targeting RNA sequence and an RNA segment that binds a protein. The system also has a nucleic acid molecule comprising a nucleic acid segment encoding a polypeptide with endonuclease activity fused to a protein that binds the RNA segment. The system also comprises a double stranded DNA molecule comprising DNA comprising at least one nucleotide sequence that is capable of binding to the target sequence at the locus.

Abstract: Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO:44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.

Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.

Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.

Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.

Abstract: Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.

Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An C aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).

Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.

Abstract: Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.

Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.