Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, using concatemers of heterologous polynucleotides that are flanked by sequences capable of sequence hybridization with a guide RNA. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a heterologous polynucleotide as a repair template.

Abstract: The disclosure relates to gene expression modulation elements from plants and their use in modulating the expression of one or more heterologous nucleic acid fragments in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds containing the expression modulating elements, and methods using the same.

Abstract: Methods and compositions to modulate maturity of a rice plant are disclosed. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds exhibiting altered stature characteristics or produce plants that exhibit altered maturity parameters.

Abstract: The present invention relates to the regulation of transgene expression in plants through a transactivation system which comprises the following: (1) a promoter comprising LexA binding sites; and (2) a fusion transactivator protein comprising a LexA DNA-binding domain and an activation domain, such as the transactivation domain of a C-repeat binding factor protein, for example, from a dehydration responsive element (DREB) factor.

Abstract: Methods are described for the identification of genes useful for conferring tolerance in plants to abiotic stress. Transgenic plants and seeds comprising the stress tolerant genes are also described. Methods to monitor the growth rate of the transgenic plants under stressed or non-stressed conditions are also described.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring stress tolerance are presented herein, along with compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP4 polypeptide.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering agronomic characteristics of plants under nitrogen limiting conditions are described. Compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs also are described. The recombinant DNA construct may comprise a polynucleotide operably linked to a heterologous promoter functional in a plant, wherein said polynucleotide encodes a PH11 or a NUCPU29 polypeptide.

Abstract: The present invention relates to the regulation of transgene expression in plants through a transactivation system which comprises the following: (1) a promoter comprising LexA binding sites; and (2) a fusion transactivator protein comprising a LexA DNA-binding domain and an activation domain, such as the transactivation domain of a C-repeat binding factor protein.

Abstract: Methods are described for the identification of genes useful for conferring tolerance in plants to abiotic stress. Transgenic plants and seeds comprising the stress tolerant genes are also described. Methods to monitor the growth rate of the transgenic plants under stressed or non-stressed conditions are also described.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a MATE-efflux polypeptide.