Abstract: The invention relates to a surface treatment method for treating the inner walls of a microchannel made from a polymeric material that is at least partially photocured or thermoset. Said treatment is carried out via irradiation in the air at a wavelength of less than or equal to 300 nm. The invention also relates to a method for manufacturing a microfluidic device including such a surface treatment step.

Abstract: A device for the microstructured grafting of proteins onto a substrate, comprising a substrate (7), a layer comprising a polyethylene glycol and being placed on the substrate, a matrix (10) of micromirrors for propagating the light in a first pattern and for replacing the first pattern with a second pattern. The microfluidic circuit is filled so as to bring a first aqueous solution containing a first protein into contact with the layer, a first microstructured image of the first pattern being formed on the layer to photoprint the first protein on the layer, and the microfluidic circuit is adapted to replace the first aqueous solution with a second aqueous solution containing a second protein so as to bring the second aqueous solution and the layer into contact, the first pattern being replaced with the second pattern in order to photoprint the second protein on the layer.

Abstract: The invention relates to a surface treatment method for treating the inner walls of a microchannel made from a polymeric material that is at least partially photocured or thermoset. Said treatment is carried out via irradiation in the air at a wavelength of less than or equal to 300 nm. The invention also relates to a method for manufacturing a microfluidic device including such a surface treatment step.

Abstract: The invention relates to a device for the microstructured grafting of a plurality of proteins onto a substrate, which includes a substrate (7), a film, a matrix (10), a light source (9), an optical system (11), a first container (1) for receiving a first aqueous solution, a second container (2) for receiving a second aqueous solution, and a microfluidic circuit, wherein the film is arranged on the substrate, the source is suitable for illuminating the matrix with the light, the matrix is suitable for propagating the light in a first structured pattern, the matrix includes an optical means for replacing the first structured pattern with a second structured pattern, the optical system is suitable for forming, on the film, a first microstructured image of the first pattern, the circuit is suitable for containing the first aqueous solution, the circuit includes an opening for placing the first solution in contact with the film at the opening, the circuit includes a microfluidic means for replacing the first solut