Patents by Inventor Amritava Das

Amritava Das has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220042048
    Abstract: Described herein are non-viral, ex vivo methods of site-specifically inserting a transgene containing a chimeric antigen receptor (CAR) gene into a T cell genome by introducing into a population of unmodified T cells a Cas9 ribonucleoprotein (RNP) and a non-viral double-stranded homology-directed repair (HDR) template, to provide genome-edited T cells. The Cas9 ribonucleoprotein includes a Cas9 protein and a guide RNA that directs double stranded DNA cleavage of a cleavage site in a T cell expressed gene. The non-viral double-stranded HDR template comprises the synthetic DNA sequence flanked by homology arms that are complementary to sequences on both sides of the cleavage site in the T cell expressed gene. The transgene is specifically integrated into the cleavage site of the T cell expressed gene created by the Cas9 RNP in the genome-edited T cells, and the cells are then cultured.
    Type: Application
    Filed: August 20, 2021
    Publication date: February 10, 2022
    Inventors: Krishanu Saha, Christian Matthew Capitini, Katherine Paige Mueller, Nicole Jenine Piscopo, Amritava Das, Matthew Hull Forsberg, Louise Armie Saraspe