Abstract: The present invention encompasses compositions and methods to allow one to deliver and express multiple genes under the control of multiple gene regulatory components in a recipient cell via a synthetic chromosome. The engineering of synthetic chromosomes to contain multiple gene control units permits the construction of complex biological circuits.

Abstract: The present invention encompasses compositions and methods to create a whole chromosome regulatable “kill” switch to inactivate expression of one or more therapeutic genes expressed from a synthetic chromosome.

Abstract: The field of the invention encompasses synthetic chromosome compositions and methods that allow single cell spatiotemporal analysis in response to differentiation cues and labeling of transplanted cells to monitor the fate and function of such cells in the patient recipient.

Abstract: The present invention encompasses compositions and methods to allow one to deliver and express multiple genes from a biosynthetic pathway in a recipient cell via a synthetic chromosome.

Abstract: Aspects of the present invention relate to the use the growth factors such as e.g., cytokines IL-2 and IL-12 for T-cell activation whereby the growth factor(s) is/are expressed by cells comprising synthetic chromosomes. The growth factor(s) is/are desirably under controllable expression from a synthetic chromosome. By approaches described herein, one can control an immune response to treat or inhibit a disease such as a cancer. The control may be provided by inducing expression of the growth factor(s), wherein expression level(s) can be fine-tuned. If more than one growth factor is expressed, the levels can be individually controlled so that the desired concentrations of each growth factor are obtained.

Abstract: The present invention provides a novel method for targeted integration of nucleic acids onto an autonomously replicating nucleic acid using site-directed recombination that allows for sequential loading of multiple delivery vectors using a single selectable marker.

Abstract: The field of the invention encompasses synthetic chromosome compositions and methods that allow single cell spatiotemporal analysis in response to differentiation cues and labeling of transplanted cells to monitor the fate and function of such cells in the patient recipient.

Abstract: Systems and methods for performing open-loop quantum error mitigation using quantum measurement emulations are provided. The open-loop quantum error mitigation methods do not require the performance of state readouts or state tomography, reducing hardware requirements and increasing overall computation speed. To perform a quantum measurement emulation, an error mitigation apparatus is configured to stochastically apply a quantum gate to a qubit or set of qubits during a quantum computational process. The stochastic application of the quantum gate projects the quantum state of the affected qubits onto an axis, reducing a trace distance between the quantum state and a desired quantum state.

Abstract: The present invention encompasses compositions and methods to allow one to deliver and express multiple genes from a biosynthetic pathway in a recipient cell via a synthetic chromosome.

Abstract: The present invention encompasses compositions and methods to rejuvenate cells by, expanding the replicative life span of the cells for, e.g., use in regenerative therapies. Specifically, the methods and compositions of the present invention increase the proliferation capacity and differentiation capacity and plasticity of cells.

Abstract: The present invention encompasses compositions and methods to rejuvenate cells by, expanding the replicative life span of the cells for, e.g., use in regenerative therapies. Specifically, the methods and compositions of the present invention increase the proliferation capacity and differentiation capacity and plasticity of cells.

Abstract: The present invention encompasses compositions and methods to allow one to monitor formation of synthetic chromosomes in real-time via standardized fluorescent technology, eliminating the need for cumbersome, expensive, and possibly mutagenic analysis.

Abstract: Systems and methods for performing open-loop quantum error mitigation using quantum measurement emulations are provided. The open-loop quantum error mitigation methods do not require the performance of state readouts or state tomography, reducing hardware requirements and increasing overall computation speed. To perform a quantum measurement emulation, an error mitigation apparatus is configured to stochastically apply a quantum gate to a qubit or set of qubits during a quantum computational process. The stochastic application of the quantum gate projects the quantum state of the affected qubits onto an axis, reducing a trace distance between the quantum state and a desired quantum state.

Abstract: The present invention encompasses compositions and methods to allow one to deliver and express multiple genes from a biosynthetic pathway in a recipient cell via a synthetic chromosome.

Abstract: The present invention encompasses compositions and methods to allow one to monitor formation of synthetic chromosomes in real-time via standardized fluorescent technology, eliminating the need for cumbersome, expensive, and possibly mutagenic analysis.

Abstract: The present invention provides a novel method for targeted integration of nucleic acids onto an autonomously replicating nucleic acid using site-directed recombination that allows for sequential loading of multiple delivery vectors using a single selectable marker.

Abstract: The present invention encompasses compositions and methods to create a whole chromosome regulatable “kill” switch to inactivate expression of one or more therapeutic genes expressed from a synthetic chromosome.

Abstract: The present invention encompasses compositions and methods to allow one to deliver and express multiple genes under the control of multiple gene regulatory components in a recipient cell via a synthetic chromosome. The engineering of synthetic chromosomes to contain multiple gene control units permits the construction of complex biological circuits.

Abstract: A wall covering assembly includes a sheet that is rolled onto itself to form a roll. An adhesive layer is coupled to the sheet and the adhesive layer adheres to a support surface thereby retaining the sheet on the support surface for ornamental purposes. A rolling unit is provided and the roll is rotatably coupled thereto thereby facilitating the sheet to be selectively drawn off of the rolling unit. The rolling unit is manipulated thereby facilitating the sheet to be applied to the support surface. A heating unit is coupled to the rolling unit and the heating unit is in thermal communication with the rolling unit. The heating unit selectively heats the rolling unit when the heating unit is turned on thereby communicating the heat to the adhesive layer for activation.

Abstract: The field of the invention encompasses synthetic chromosome compositions and methods that allow single cell spatiotemporal analysis in response to differentiation cues and labeling of transplanted cells to monitor the fate and function of such cells in the patient recipient.