Abstract: The invention relates to a recombinant Marasmius rotula unspecific peroxygenase (rMroURO) and two mutants thereof, wherein said mutants show enhanced selectivity towards either the epoxidation or the (sub)terminal ?/(?-1)-hydroxylation of unsaturated fatty acids. The invention also refers to the use of these enzyme variants for the specific epoxidation or hydroxylation of fatty acids such as oleic acid, linoleic acid and/or alpha-linolenic acid.

Abstract: The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of two ends of a substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

Abstract: The invention relates to the use of mediator-enzyme system, which is characterised in that the enzyme comprises a laccase-type enzyme and the mediator comprises a chemical compound which acts as a redox intermediate in the enzymatic oxidation, for the elimination of lipophilic compounds (including, among others, sterols that are free and conjugated in the form of esters and glycosides, triglycerides, fatty alcohols and resin acids) which cause deposits in the product, the machinery and the circuits during the production of pulp and paper (both from hardwood and softwood and non-woody plants). The treatment eliminates up to 100% of the aforementioned lipophilic compounds, depending on the material treated, and, in this way, improves the quality of the pulp obtained, the operation of the industrial installations and the characteristics of the end product.