Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

Abstract: A coated ultrafiltration device comprises an upper chamber; a lower chamber; a filtration chamber disposed between the upper chamber and lower chamber; and a semipermeable membrane disposed substantially vertically around the filtration chamber. The semipermeable membrane comprises a coating on a portion of the semipermeable membrane exposed to the filtration chamber, wherein the coating comprises an ultra-low attachment coating that is not derived from animal sources.

Abstract: A cell culture vessel (100) includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate (215) of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (220), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.

Abstract: The present disclosure relates to agarose and methylcellulose storage and transport mediums, systems for cell storage and transport, and cell storage and transport methods. The instantly-disclosed agarose and methylcellulose storage and transport medium is ideally suited for 3D spheroid cell culture storage and transport. In particular aspects, the storage and transport mediums, systems, and methods are used in combination with or performed in labware that combine 3D spheroid culture with a gas permeable, micro-patterned design that allows for protection and prolonged maintenance of spheroid cell (e.g. hepatocytes) viability and functionality during storage and transport.

Abstract: A pipette structure (160B) includes first and second body sections (164, 166) connected at a junction and including first and second fluid passages, respectively, with the pipette structure including (i) a bellows joint (165) permitting pivotal movement between the first and second body sections (164, 165), and/or (ii) the second fluid passage of the second body section includes greater width dimension at a sample introduction end than a width dimension at the junction. Such a pipette structure may be embodied in a unitary measuring pipette or an extension tip device for a measuring pipette. A method is provided for transferring material between an interior of a cell culture housing that includes a structured surface defining an array of microwells suitable for three-dimensional culture of multi-cell aggregates and an external environment. A method for fabricating a pipette structures is further provided.

Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

Abstract: A cell culture vessel (100) includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate (215) of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (220), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.

Abstract: An article including, either: a frame having an interior cavity, and a flexible liner situated within the interior cavity; or a deformable frame having an interior cavity, as defined herein. Either frame can receive a sample, such as liquid having suspended live cells, and cooling freezes the sample to a solid sample. The solid sample can be readily ejected from the article having either a frame with the flexible liner, or the deformable frame, without extensive thawing. The solid sample can be rapidly displaced from the article by the methods and apparatus disclosed herein.