Patents by Inventor Ana Negrete-Raymond
Ana Negrete-Raymond has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240018464Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.Type: ApplicationFiled: June 15, 2023Publication date: January 18, 2024Applicant: CARGILL, INCORPORATEDInventors: Kenneth R. FINLEY, Holly JESSEN, Erin Kathleen MARASCO, Thomas William MCCMULLIN, Ana NEGRETE-RAYMOND, Amit VAS
-
Patent number: 11718820Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.Type: GrantFiled: August 2, 2018Date of Patent: August 8, 2023Assignee: CARGILL, INCORPORATEDInventors: Kenneth R. Finley, Holly Jessen, Erin Kathleen Marasco, Thomas William McMullin, Ana Negrete-Raymond, Amit Vas
-
Patent number: 11591620Abstract: A system for editing of a target sequence at a locus of a host cell is disclosed. The system has a nucleic acid molecule comprising a nucleic acid segment comprising a targeting RNA sequence and an RNA segment that binds a protein. The system also has a nucleic acid molecule comprising a nucleic acid segment encoding a polypeptide with endonuclease activity fused to a protein that binds the RNA segment. The system also comprises a double stranded DNA molecule comprising DNA comprising at least one nucleotide sequence that is capable of binding to the target sequence at the locus.Type: GrantFiled: May 18, 2018Date of Patent: February 28, 2023Assignee: CARGILL, INCORPORATEDInventors: Kenneth R. Finley, Briana Kozlowicz, Ana Negrete-Raymond, Gregory M. Poynter, Amit Vas
-
Publication number: 20220403425Abstract: Genes encoding mutant 3-ketoacyl-CoA synthases are introduced into host cells. Certain of the mutants enhance the production of shorter-chain fatty acids and derivatives by the cell than do the wild-type (unmutated) enzymes. In other cases, the chain length is not significantly affected, but productivity is enhanced. In specific cases, both a shift toward lower chain length and higher productivity is seen. Cells producing the mutant 3-ketoacyl-CoA synthases are especially suitable for producing C6-C10 fatty acids and derivatives.Type: ApplicationFiled: May 2, 2022Publication date: December 22, 2022Applicant: CARGILL, INCORPORATEDInventors: Hans H. LIAO, Catherine Bradshaw POOR, Travis Robert WOLTER, Michael Tai Man LOUIE, Erin Kathleen MARASCO, Ana NEGRETE-RAYMOND
-
Publication number: 20220177926Abstract: A system for editing of a target sequence at a locus of a host cell is disclosed. The system has a nucleic acid molecule comprising a nucleic acid segment comprising a targeting RNA sequence and an RNA segment that binds a protein. The system also has a nucleic acid molecule comprising a nucleic acid segment encoding a polypeptide with endonuclease activity fused to a protein that binds the RNA segment. The system also comprises a double stranded DNA molecule comprising DNA comprising at least one nucleotide sequence that is capable of binding to the target sequence at the locus.Type: ApplicationFiled: May 18, 2018Publication date: June 9, 2022Applicant: CARGILL, INCORPORATEDInventors: Kenneth R. FINLEY, Briana KOZLOWICZ, Ana NEGRETE-RAYMOND, Gregory M. POYNTER, Amit VAS
-
Patent number: 11345938Abstract: Genes encoding mutant 3-ketoacyl-CoA synthases are introduced into host cells. Certain of the mutants enhance the production of shorter-chain fatty acids and derivatives by the cell than do the wild-type (unmutated) enzymes. In other cases, the chain length is not significantly affected, but productivity is enhanced. In specific cases, both a shift toward lower chain length and higher productivity is seen. Cells producing the mutant 3-ketoacyl-CoA synthases are especially suitable for producing C6-C10 fatty acids and derivatives.Type: GrantFiled: February 1, 2018Date of Patent: May 31, 2022Assignee: CARGILL, INCORPORATEDInventors: Hans H. Liao, Catherine Bradshaw Poor, Travis Robert Wolter, Michael Tai Man Louie, Erin Kathleen Marasco, Ana Negrete-Raymond
-
Publication number: 20200308613Abstract: A recombinant cell for producing a fatty acid ester. The recombinant cell is genetically engineered to produce a reduction in free fatty acids compared to a cell that has not been similarly genetically engineered. Methods for producing fatty acid esters while decreasing free fatty acid production are also described.Type: ApplicationFiled: April 1, 2020Publication date: October 1, 2020Applicant: CARGILL, INCORPORATEDInventors: Michael Tai Man LOUIE, Ana NEGRETE-RAYMOND, Travis Robert WOLTER
-
Publication number: 20200270644Abstract: Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO:44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.Type: ApplicationFiled: December 16, 2016Publication date: August 27, 2020Inventors: CHRISTOPHER K. MILLER, Ana Negrete-Raymond, Brian J. Rush, Amit Vas, Jon Veldhouse
-
Patent number: 10724023Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.Type: GrantFiled: July 15, 2019Date of Patent: July 28, 2020Assignee: CARGILL, INCORPORATEDInventors: Christopher K. Miller, Ana Negrete-Raymond, Jonathan Dwight Veldhouse, Amit Vas
-
Publication number: 20200172855Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.Type: ApplicationFiled: August 2, 2018Publication date: June 4, 2020Applicant: CARGILL, INCORPORATEDInventors: Kenneth R. FINLEY, Holly JESSEN, Erin Kathleen MARASCO, Thomas William MCCMULLIN, Ana NEGRETE-RAYMOND, Amit VAS
-
Publication number: 20200048623Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.Type: ApplicationFiled: July 15, 2019Publication date: February 13, 2020Applicant: CARGILL, INCORPORATEDInventors: Christopher K. MILLER, Ana NEGRETE-RAYMOND, Jon VELDHOUSE, Amit VAS
-
Publication number: 20190376071Abstract: Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.Type: ApplicationFiled: June 12, 2019Publication date: December 12, 2019Applicant: CARGILL, INCORPORATEDInventors: Christopher K. Miller, Ana Negrete-Raymond, Jon Veldhouse, Amit Vas
-
Publication number: 20190360010Abstract: Genes encoding mutant 3-ketoacyl-CoA synthases are introduced into host cells. Certain of the mutants enhance the production of shorter-chain fatty acids and derivatives by the cell than do the wild-type (unmutated) enzymes. In other cases, the chain length is not significantly affected, but productivity is enhanced. In specific cases, both a shift toward lower chain length and higher productivity is seen. Cells producing the mutant 3-ketoacyl-CoA synthases are especially suitable for producing C6-C10 fatty acids and derivatives.Type: ApplicationFiled: February 1, 2018Publication date: November 28, 2019Applicant: GARGILL, INCORPORATEDInventors: Hans H. LIAO, Catherine Bradshaw POOR, Travis Robert WOLTER, Michael Tai Man LOUIE, Erin Kathleen MARASCO, Ana NEGRETE-RAYMOND
-
Patent number: 10364421Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.Type: GrantFiled: February 5, 2016Date of Patent: July 30, 2019Assignee: CARGILL, INCORPORATEDInventors: Christopher K. Miller, Ana Negrete-Raymond, Jon Veldhouse, Amit Vas
-
Patent number: 10344288Abstract: Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.Type: GrantFiled: March 25, 2016Date of Patent: July 9, 2019Assignee: CARGILL, INCORPORATEDInventors: Christopher K. Miller, Ana Negrete-Raymond, Jon Veldhouse, Amit Vas
-
Publication number: 20180094269Abstract: Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.Type: ApplicationFiled: March 25, 2016Publication date: April 5, 2018Inventors: Christopher K. MILLER, Ana NEGRETE-RAYMOND, Jon VELDHOUSE, Amil VAS
-
Publication number: 20180080014Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.Type: ApplicationFiled: February 5, 2016Publication date: March 22, 2018Inventors: Christopher K. Miller, Ana NEGRETE-RAYMOND, Jon VELDHOUSE, Amit VAS
-
Patent number: 9862975Abstract: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.Type: GrantFiled: September 18, 2015Date of Patent: January 9, 2018Assignee: CARGILL, INCORPORATEDInventors: Holly J. Jessen, Jian Yi, Joshua Lundorff, Hans Liao, Ana Negrete-Raymond, Pirkko Suominen, Aristos Aristidou
-
Patent number: 9845484Abstract: Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding an aspartate 1-decarboxylase (ADC) of the Class Insecta, Bivalvia, Branchioporia, Gastropoda, or Leptocardii. Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid.Type: GrantFiled: July 31, 2014Date of Patent: December 19, 2017Assignee: NOVOZYMES A/SInventors: Michelle Barnhart, Ana Negrete-Raymond, Janice Frias, Gui Barbier, Michael Catlett
-
Publication number: 20160168600Abstract: Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding an aspartate 1-decarboxylase (ADC) of the Class Insecta, Bivalvia, Branchioporia, Gastropoda, or Leptocardii. Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid.Type: ApplicationFiled: July 31, 2014Publication date: June 16, 2016Applicant: NOVOZYMES A/SInventors: Michelle Barnhart, Ana Negrete-Raymond, Janice Frias, Gui Barbier, Michael Catlett