Abstract: Assays, kits, and methods that target and/or detect the presence of bovine respiratory complex (BRD)-associated pathogens in a sample. These assays, kits and methods can be portable and capable of providing fast (within 45 minutes) and accurate (at least 96%) results in the field, eliminating the need for a laboratory and other complex equipment.

Abstract: The present disclosure is drawn to methods of preparing a saliva sample for loop-mediated isothermal amplification (LAMP) detection of a pathogen target. In some embodiments, such methods can include providing an amount of saliva from a test subject, and diluting the saliva in water to a degree that reduces a buffering capacity of the saliva while maintaining a sufficient concentration to allow for detection of the pathogen target.