Abstract: The present invention relates to process of enrichment of Boron isotopes by ion exchange chromatography by “Auto Cut” system using continuous Ion Exchange Chromatography. The process comprises development of borate band in the ion exchange chromatographic column; eluting said band without any regeneration in a manner where said ion exchange chromatographic columns comprises 3-9 chromatographic columns in groups of three columns each and connected in series with silicon tubes; and wherein said eluting agent is provided to first column of each said group of three columns; and wherein said enriched boron isotopes are obtained upto 90%.