Abstract: The present invention relates to an anti-HIV recombinant HIV-1 derived Topoisomerase II? kinase. It inhibits HIV-1 replication by blocking viral entry. Its recognition by envelope antibodies ID6 and 4G10 makes it a justifiable immunogen for use as vaccine candidate in form of protein, mRNA and DNA vaccine against HIV infection. Thus, the protein, mRNA and DNA of immunogenic recombinant HIV-1 derived Topoisomerase II? kinase and derived peptides with and without spacers can be used as a HIV vaccine. FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7, FIG. 7, FIG. 8, FIG. 9, FIG. 10, FIG. 11, FIG. 12, FIG. 13, FIG. 14, FIG. 15, FIG. 16.