Abstract: Apparatuses may be used for nucleic acid amplification. An example apparatus may include an amplification chamber to contain a biochemical reaction associated with amplification of a biologic sample. The biologic sample may include a nucleic acid. The apparatus may also include a heater thermally coupled to the amplification chamber. The apparatus may also include a thermally conductive substrate in contact with the heater and including a liquid coolant passage to pass a liquid coolant through the thermally conductive substrate.

Abstract: A microfluidic device includes a first channel, second channels, and a transition channel splitting the first channel into the second channels. The transition has a first end fluidically connected to the first channel and a second end fluidically connected to the second channels. The transition channel expands in width from a width of the first channel at the first end to no less than a sum of widths of the second channels at the second end so as to promote fluid flow from the first channel to the second channels.

Abstract: A microfluidic device includes a first channel having a first width and a second channel having a second width greater than the first width. The microfluidic device includes a transition channel having a first end fluidically connected to the first channel and a second end fluidically connected to the second channel. The transition channel expands in width from the first width to the second width so as to promote fluid flow from the first channel to the second channel.

Abstract: A microfluidic device includes a chamber having sidewalls, a floor, a ceiling, and an inlet. The microfluidic device includes pillars extending from the floor to the ceiling of the chamber. Each pillar has an orientation relative to the inlet defined by a leading surface and a trailing corner opposite the leading corner. The trailing corner has an angle less than a threshold angle that is based on a fluidic contact angle. The orientations of the pillars relative to the inlet promote fluid flow from the inlet throughout the chamber without trapping gas at the sidewalls of the chamber.

Abstract: An example microfluidic mixer can include an inlet microfluidic channel portion and a fluid splitting channel portion including an overpass microfluidic channel to receive fluid from a first side of the inlet microfluidic channel portion and an underpass microfluidic channel to receive fluid from a second side of the inlet microfluidic channel portion, where the underpass microfluidic channel extends under the overpass microfluidic channel such that the channels overlap at their respective downstream ends. A fluid recombining channel portion is downstream of the fluid splitting portion and includes an angled recombining surface having an acute angle with respect to a direction of fluid flow, where the angled recombining surface is between the downstream ends of the overpass and underpass microfluidic channels. An outlet microfluidic channel portion is fluidly connected downstream from the fluid recombining channel portion.

Abstract: An example microfluidic device comprises a plurality of fluidic channels and a fluidic multiplexor. The fluidic multiplexor includes a plurality of fluidic micro-valves fluidically coupled to the plurality of fluidic channels, and a plurality of control lines that cross the plurality of fluidic channels proximal to the plurality of fluidic micro-valves.

Abstract: An example microfluidic structure can include a first microfluidic channel segment in a first elevation plane, a second microfluidic channel segment in a second elevation plane, and a transverse microfluidic channel segment connecting the first microfluidic channel segment to the second microfluidic channel segment. An angled exterior wall segment can be at the transverse microfluidic channel segment. The angled exterior wall segment can be angled in the first or second elevation plane at an acute angle with respect to a direction of fluid flow through the first or second microfluidic channel segment. A fluid cross-sectional area can increase in the fluid flow direction along the angled exterior wall segment.

Abstract: An example microfluidic structure can include a first microfluidic channel segment in a first elevation plane, a second microfluidic channel segment in a second elevation plane, and a transverse microfluidic channel segment connecting the first microfluidic channel segment to the second microfluidic channel segment. An interior pillar can be positioned at the transverse microfluidic channel segment. The interior pillar can have a tapered downstream edge. The tapered downstream edge can be angled in the first or second elevation plane at an acute angle. A fluid cross-sectional area can increase in the fluid flow direction along the tapered downstream edge.

Abstract: An example microfluidic device comprises a plurality of fluidic channels and a fluidic multiplexor. The fluidic multiplexor includes a plurality of fluidic micro-valves fluidically coupled to the plurality of fluidic channels, and a plurality of control lines that cross the plurality of fluidic channels proximal to the plurality of fluidic micro-valves.