Patents by Inventor Anandita SETH

Anandita SETH has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • AUTOMATED PRODUCTION OF VIRAL VECTORS
    Publication number: 20250066744
    Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.
    Type: Application
    Filed: November 8, 2024
    Publication date: February 27, 2025
    Inventors: Matthew HEWITT, Young SHIN, Bingnan GU, Caitlin M. GUENTHER, Anandita SETH
  • Automated production of viral vectors
    Patent number: 12180511
    Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.
    Type: Grant
    Filed: June 20, 2023
    Date of Patent: December 31, 2024
    Assignee: LONZA WALKERSVILLE, INC
    Inventors: Matthew Hewitt, Young Shin, Bingnan Gu, Caitlin M. Guenther, Anandita Seth
  • METHODS AND CONSTRUCTS FOR PRODUCTION OF LENTIVIRAL VECTOR
    Publication number: 20240336936
    Abstract: The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.
    Type: Application
    Filed: May 31, 2024
    Publication date: October 10, 2024
    Inventors: Young SHIN, Anandita SETH, Bingnan GU
  • Methods and constructs for production of lentiviral vector
    Patent number: 12037599
    Abstract: The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.
    Type: Grant
    Filed: August 24, 2020
    Date of Patent: July 16, 2024
    Assignee: LONZA WALKERSVILLE, INC.
    Inventors: Young Shin, Anandita Seth, Bingnan Gu
  • AUTOMATED PRODUCTION OF VIRAL VECTORS
    Publication number: 20240026306
    Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.
    Type: Application
    Filed: June 20, 2023
    Publication date: January 25, 2024
    Inventors: Matthew HEWITT, Young SHIN, Bingnan GU, Caitlin M. GUENTHER, Anandita SETH
  • ADENO-ASSOCIATED VIRUS (AAV) PRODUCER CELL LINE AND RELATED METHODS
    Publication number: 20240018545
    Abstract: The present disclosure relates to a mammalian cell line for producing adeno-associated virus (AAV), suitably including nucleic acids encoding helper genes and AAV genes, under the control of derepressible promoters. The disclosure also relates to isolated nucleic acid molecules that encode such genes, as well as methods of using the mammalian cells for producing AAVs.
    Type: Application
    Filed: July 14, 2023
    Publication date: January 18, 2024
    Inventors: Bingnan GU, Caitlin M. GUENTHER, Anandita SETH
  • Mammalian cells for producing adeno-associated viruses
    Patent number: 11781116
    Abstract: A mammalian cell comprising at least four distinct recombination target sites (RTS), an adenovirus (Ad) gene comprising E1A, E1B or a combination thereof, and a promoter operatively linked to the Ad gene, wherein the RTS, the Ad gene, and the promoter are chromosomally-integrated; methods for using the cell for generating a recombinant adeno-associated virus (rAAV) producer host cell; and methods for using the AAV producer host cell to produce, package and purify rAAV.
    Type: Grant
    Filed: February 17, 2018
    Date of Patent: October 10, 2023
    Assignee: LONZA LTD.
    Inventors: Marc Feary, Robert J. Young, Anandita Seth
  • Adeno-associated virus (AAV) producer cell line and related methods
    Patent number: 11739347
    Abstract: The present disclosure relates to a mammalian cell line for producing adeno-associated virus (AAV), suitably including nucleic acids encoding helper genes and AAV genes, under the control of derepressible promoters. The disclosure also relates to isolated nucleic acid molecules that encode such genes, as well as methods of using the mammalian cells for producing AAVs.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: August 29, 2023
    Assignee: LONZA WALKERVILLE, INC.
    Inventors: Bingnan Gu, Caitlin M. Guenther, Anandita Seth
  • Automated production of viral vectors
    Patent number: 11718833
    Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: August 8, 2023
    Assignee: LONZA WALKERSVILLE, INC.
    Inventors: Matthew Hewitt, Young Shin, Bingnan Gu, Caitlin M. Guenther, Anandita Seth
  • METHODS AND KITS FOR DETECTING ADENO-ASSOCIATED VIRUSES
    Publication number: 20230168250
    Abstract: The present disclosure provides methods and kits for detecting adeno-associated viruses. In some embodiments, the adeno-associated virus is Anc80. Detection methods include HPLC- and ELISA-based methods.
    Type: Application
    Filed: May 10, 2021
    Publication date: June 1, 2023
    Inventors: Peng WANG, Bingnan GU, Anandita SETH
  • METHODS OF DETERMINING VIRAL TITER
    Publication number: 20230038151
    Abstract: The present disclosure relates to methods for determining a viral titer of a biological sample, suitably from a mammalian cell sample. The methods include the use of mechanical disruption of the cells, followed by droplet digital polymerase chain reaction (ddPCR) to determine the viral titer. Methods of mechanical disruption suitably include the use of glass beads.
    Type: Application
    Filed: January 26, 2021
    Publication date: February 9, 2023
    Inventors: Young SHIN, Anandita SETH
  • METHODS AND CONSTRUCTS FOR TRANSIENT PRODUCTION OF LENTIVIRAL VECTOR
    Publication number: 20230026345
    Abstract: The present disclosure relates to methods for producing lentiviral vectors using mammalian cells. Specifically, the methods utilize three plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral vectors in mammalian cells, including suspension-based cells. These methods allow for the production of lentiviral vectors that can be tailored to include a specific gene of interest.
    Type: Application
    Filed: December 17, 2020
    Publication date: January 26, 2023
    Inventors: Young SHIN, Anandita SETH
  • Methods for isolating adeno-associated virus using a polydialkylammonium salt
    Patent number: 11427809
    Abstract: The invention relates to methods of selectively purifying an adeno-associated virus (AAV) from an aqueous biomass using a flocculent. In embodiments, the flocculent is polydiallyldialkylammonium salt, e.g., polydiallyldimethvlammonium chloride (pDADMAC).
    Type: Grant
    Filed: May 25, 2017
    Date of Patent: August 30, 2022
    Assignee: LONZA HOUSTON, INC.
    Inventors: Wenling Dong, Anandita Seth, Robert J. Milczarek, Francesca P. Vitelli
  • METHODS AND CONSTRUCTS FOR PRODUCTION OF LENTIVIRAL VECTOR
    Publication number: 20210062220
    Abstract: The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.
    Type: Application
    Filed: August 24, 2020
    Publication date: March 4, 2021
    Inventors: Young SHIN, Anandita SETH, Bingnan GU
  • METHODS FOR ISOLATING ADENO-ASSOCIATED VIRUS USING A POLYDIALKYLAMMONIUM SALT
    Publication number: 20200407695
    Abstract: The invention relates to methods of selectively purifying an adeno-associated virus (AAV) from an aqueous biomass using a flocculent. In embodiments, the flocculent is polydiallyldialkylammonium salt, e.g., polydiallyldimethvlammonium chloride (pDADMAC).
    Type: Application
    Filed: May 25, 2017
    Publication date: December 31, 2020
    Inventors: Wenling DONG, Anandita SETH, Robert J. MILCZAREK, Francesca P. VITELLI
  • AUTOMATED PRODUCTION OF VIRAL VECTORS
    Publication number: 20200208121
    Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.
    Type: Application
    Filed: December 19, 2019
    Publication date: July 2, 2020
    Inventors: Matthew HEWITT, Young SHIN, Bingnan GU, Caitlin M. GUENTHER, Anandita SETH
  • ADENO-ASSOCIATED VIRUS (AAV) PRODUCER CELL LINE AND RELATED METHODS
    Publication number: 20200199627
    Abstract: The present disclosure relates to a mammalian cell line for producing adeno-associated virus (AAV), suitably including nucleic acids encoding helper genes and AAV genes, under the control of derepressible promoters. The disclosure also relates to isolated nucleic acid molecules that encode such genes, as well as methods of using the mammalian cells for producing AAVs.
    Type: Application
    Filed: December 18, 2019
    Publication date: June 25, 2020
    Inventors: Bingnan GU, Caitlin M. GUENTHER, Anandita SETH
  • MAMMALIAN CELLS FOR PRODUCING ADENO-ASSOCIATED VIRUSES
    Publication number: 20200002682
    Abstract: A mammalian cell comprising at least four distinct recombination target sites (RTS), an adenovirus (Ad) gene comprising E1A, E1B or a combination thereof, and a promoter operatively linked to the Ad gene, wherein the RTS, the Ad gene, and the promoter are chromosomally-integrated; methods for using the cell for generating a recombinant adeno-associated virus (rAAV) producer host cell; and methods for using the AAV producer host cell to produce, package and purify rAAV.
    Type: Application
    Filed: February 17, 2018
    Publication date: January 2, 2020
    Inventors: Marc FEARY, Robert J. YOUNG, Anandita SETH