Patents by Inventor Anant Y. Patkar

Anant Y. Patkar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Apparatus and methods for osmotically shocking cells
    Patent number: 8211668
    Abstract: A method of preparing a recombinant polypeptide of interest includes fermenting a host cell being transformed with a recombinant expression system capable of bringing about secretion of a polypeptide of interest into the periplasm of said host cell. The polypeptide of interest is extracted from the periplasm by applying a continuous osmotic shock to the host cells contained in a fermentation medium. An apparatus for osmotically shocking cells includes a first reservoir containing cells in a first solution and a second reservoir containing a second solution, the first solution having a higher osmolarity than the second solution. A method for osmotically shocking cells using the first and second solutions is also disclosed. Also disclosed is a method of isolating a recombinant polypeptide of interest from a cell.
    Type: Grant
    Filed: January 11, 2008
    Date of Patent: July 3, 2012
    Assignee: Dow AgroSciences, LLC.
    Inventors: Anant Y. Patkar, Subrata Sen, Micheal L. Chappell
  • APPARATUS AND METHODS FOR OSMOTICALLY SHOCKING CELLS
    Publication number: 20080182295
    Abstract: A method of preparing a recombinant polypeptide of interest includes fermenting a host cell being transformed with a recombinant expression system capable of bringing about secretion of a polypeptide of interest into the periplasm of said host cell. The polypeptide of interest is extracted from the periplasm by applying a continuous osmotic shock to the host cells contained in a fermentation medium. An apparatus for osmotically shocking cells includes a first reservoir containing cells in a first solution and a second reservoir containing a second solution, the first solution having a higher osmolarity than the second solution. A method for osmotically shocking cells using the first and second solutions is also disclosed. Also disclosed is a method of isolating a recombinant polypeptide of interest from a cell.
    Type: Application
    Filed: January 11, 2008
    Publication date: July 31, 2008
    Inventors: Anant Y. Patkar, Subrata Sen, Micheal L. Chappell