Abstract: This invention relates to methods of obtaining volatile hydrocarbons produced by photosynthetic microorganisms using a two-phase gaseous/aqueous bioreactor.

Abstract: This invention relates to a new method of selecting for transgene transformants in the absence of antibiotic selective pressure, where the method is based on recovery of function.

Abstract: The invention provides methods of extracting and quantifying extracellular terpenoid hydrocarbons, e.g., botryococcenes, methylated squalenes, and carotenoids, from terpenoid-producing and secreting green microalgae.

Abstract: The present invention provides methods and compositions for producing isoprene hydrocarbons from cyanobacteria.

Abstract: The present invention provides methods and compositions for producing isoprene hydrocarbons from microalgae, cyanobacteria, and photosynthetic and non-photosynthetic bacteria.

Abstract: The present invention provides methods and compositions for producing isoprene hydrocarbons from microalgae, cyanobacteria, and photosynthetic and non-photosynthetic bacteria.

Abstract: The present invention provides methods and compositions for producing isoprene hydrocarbons from microalgae, cyanobacteria, and photosynthetic and non-photosynthetic bacteria.

Abstract: The present invention provides methods and compositions for producing isoprene hydrocarbons from Ascomycota such as yeast and filamentous fungi.

Abstract: The invention provides method and compositions to minimize the chlorophyll antenna size of photosynthesis by decreasing TLA1 gene expression, thereby improving solar conversion efficiencies and photosynthetic productivity in plants, e.g., green microalgae, under bright sunlight conditions.

Abstract: The invention provides method and compositions to minimize the chlorophyll antenna size of photosynthesis by decreasing TLA1 gene expression, thereby improving solar conversion efficiencies and photosynthetic productivity in plants, e.g., green microalgae, under bright sunlight conditions.

Abstract: A method for determining the content of a bioproduct in a cell culture comprises (a) loading a sample of the cell culture onto a density gradient comprising a density determination agent; (b) centrifuging the product of step (a) for a period of time sufficient to establish a density equilibrium between the cell culture sample and the density gradient; (c) measuring the density of the cell culture sample containing the bioproduct based on its density equilibrium, and (d) calculating the weight percent of the bioproduct in the cell culture using the equations: ?S=(x·?P)+(y·?B) x+y=1 wherein: ?S represents the density of the cell culture sample containing the bioproduct (in g/mL); ?P represents the density of the bioproduct in pure form (in g/mL); ?B represents the density of the cell biomass in the culture devoid of bioproduct (in g/mL); x represents the weight % of the bioproduct in the cell culture; and y represents the weight % of the cell biomass in the cell culture.

Abstract: The invention provides method and compositions to minimize the chlorophyll antenna size of photosynthesis by decreasing TLA1 gene expression, thereby improving solar conversion efficiencies and photosynthetic productivity in plants, e.g., green microalgae, under bright sunlight conditions.

Abstract: The present invention provides methods and compositions for producing isoprene hydrocarbons from microalgae, cyanobacteria, and photosynthetic and non-photosynthetic bacteria.

Abstract: Sustained hydrogen production is obtained by the culturing of a genetically-modified algae, where the ability of the chloroplasts to intake sulfate is reduced or eliminated compared to wild-type algae. The alga is cultured in a sealed environment in a liquid or solid medium that contains sulfur, and hydrogen is generated continuously. Alternatively, the algae may be cultured in the presence of bacteria that also produce hydrogen gas. The hydrogen produced can be collected and used as a clean energy source.

Abstract: A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.

Abstract: Sustained hydrogen production is obtained by the culturing of a genetically-modified algae, where the ability of the chloroplasts to intake sulfate is reduced or eliminated compared to wild-type algae. The alga is cultured in a sealed environment in a liquid or solid medium that contains sulfur, and hydrogen is generated continuously. Alternatively, the algae may be cultured in the presence of bacteria that also produce hydrogen gas. The hydrogen produced can be collected and used as a clean energy source.

Abstract: Sustained hydrogen production is obtained by the culturing of a genetically-modified algae, where the ability of the chloroplasts to intake sulfate is reduced or eliminated compared to wild-type algae. The alga is cultured in a sealed environment in a liquid or solid medium that contains sulfur, and hydrogen is generated continuously. Alternatively, the algae may be cultured in the presence of bacteria that also produce hydrogen gas. The hydrogen produced can be collected and used as a clean energy source.