Abstract: An apparatus for separation of chemical substances and production of sterile solutions, which comprises a column closed with plugs at the ends thereof and containing an ion-exchange agent and at least one filter. In the body of each plug there is a piercing duct with an inner expansion chamber wherein a resilient stopper is placed, which stopper is provided with longitudinal notches at its external surface extending over the end surface of the stopper facing the column so that said longitudinal notches communicate with the piercing duct of the plug. At the opposite end of the stopper there is a closing cone abutting with its surface upon the edges of the end portion of the piercing duct thus ensuring tight sealing of the latter.