Patents by Inventor Anders Ljunglof
Anders Ljunglof has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11571635Abstract: The present invention relates to a liquid chromatography system for the separation of bio-molecules in a fluid including at least two unit operations, wherein the first unit operation is a step of multi-column chromatography and the second unit operation is a step modifying said bio-molecules and/or the fluid, wherein the modification comprises feeding the fluid resulting from the last chromatography column of the first unit operation into a system comprising at least two containers, wherein each container has a volume and a moveable sidewall arranged to divide the volume into a first sub-volume and a second sub-volume, and each container comprises a first port connected to the first volume and a second port connected to the second sub-volume. The invention also relates to a virus inactivation device for a chromatography system according to the invention, which enables continuous or semi-continuous processing of biomolecules, as well as a method of using such a device.Type: GrantFiled: March 23, 2016Date of Patent: February 7, 2023Assignee: Cytiva Sweden ABInventors: Bjorn Markus Olovsson, Anders Ljunglof, Karol Maciej Lacki
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Publication number: 20180111059Abstract: The present invention relates to a liquid chromatography system for the separation of bio-molecules in a fluid including at least two unit operations, wherein the first unit operation is a step of multi-column chromatography and the second unit operation is a step modifying said bio-molecules and/or the fluid, wherein the modification comprises feeding the fluid resulting from the last chromatography column of the first unit operation into a system comprising at least two containers, wherein each container has a volume and a moveable sidewall arranged to divide the volume into a first sub-volume and a second sub-volume, and each container comprises a first port connected to the first volume and a second port connected to the second sub-volume. The invention also relates to a virus inactivation device for a chromatography system according to the invention, which enables continuous or semi-continuous processing of biomolecules, as well as a method of using such a device.Type: ApplicationFiled: March 23, 2016Publication date: April 26, 2018Inventors: Bjorn Markus Olovsson, Anders Ljunglof, Karol Maciej Lacki
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Publication number: 20180036445Abstract: The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of: a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support, b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I, R—O—O—H (I) wherein R is hydrogen or an acyl group R?—C(O)—, with R? being a hydrogen or a methyl, ethyl or propyl group.Type: ApplicationFiled: February 25, 2016Publication date: February 8, 2018Inventors: Elin Marianne Monie, Tomas Bjorkman, Anna Gronberg, Anders Ljunglof, Gustav Jose Rodrigo, Karin Torstenson, Magnus Carl Erik Wetterhall
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Publication number: 20120149875Abstract: The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods being that each of the ligands consists essentially of a monomer or dimer SpA or protein Z or a functional variant thereof.Type: ApplicationFiled: January 11, 2010Publication date: June 14, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Hans J. Johansson, Anders Ljunglof, Ronnie Palmgren
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Patent number: 8070958Abstract: The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.Type: GrantFiled: January 25, 2010Date of Patent: December 6, 2011Assignee: GE Healthcare Bio-Sciences ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Anders Ljunglof, Jean-Luc Maloisel
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Publication number: 20100130354Abstract: The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.Type: ApplicationFiled: January 25, 2010Publication date: May 27, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: JAN BERGSTROM, GUNNAR GLAD, BO-LENNART JOHANSSON, ANDERS LJUNGLOF, JEAN-LUC MALOISEL
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Publication number: 20080230478Abstract: The present invention relates to a process of regenerating a separation matrix, such as a chromatography matrix, comprising adsorption of at least one target molecule by contacting a mobile phase comprising at target molecule(s) with a matrix; removal of unbound material by washing the matrix; elution of target molecule(s) by contacting the matrix with an eluent; reducing regeneration by contacting said matrix with a reducing agent; alkaline regeneration by contacting the matrix with an alkaline solution; and equilibration of the matrix.Type: ApplicationFiled: May 22, 2006Publication date: September 25, 2008Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Hans Johansson, Anders Ljunglof, Per-Mikael Aberg
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Patent number: 7385040Abstract: The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.Type: GrantFiled: February 24, 2005Date of Patent: June 10, 2008Assignee: GE Healthcare Bio-Sciences ABInventors: Bo-Lennart Johansson, Hans J. Johansson, Anders Ljunglöf, Jean-Luc Maloisel, Nicolas Thevenin
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Publication number: 20070213513Abstract: The present invention relates to a method of isolating a target compound from other components of a liquid, which method comprises at least two chromatographic steps, in any sequence of order, wherein the mobile phase is contacted with an affinity chromatography matrix and/or an ion-exchange chromatography matrix and/or a hydrophobic interaction chromatography matrix, wherein the contacting with at least one of the matrices takes place in the presence of at least one non-ionic polyether; and obtaining the target compound(s) in a separate fraction from the last chromatographic step. In the most preferred embodiment, the non-ionic polyether is poly(ethylene glycol) (PEG).Type: ApplicationFiled: March 31, 2005Publication date: September 13, 2007Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: James Van Alstine, Hamid Houshmand, Anders Ljunglof, Per-Mikael Aberg
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Publication number: 20070161117Abstract: The present invention relates to a method for reducing total sample complexity in native or digested biological sample(s), before analysis thereof by mass spectrometry, comprising the following steps: a) selecting a fraction from the entire native or digested biological sample(s) on the basis of pi-value, said fraction comprising native or digested sample representing a subset of or the entire substance population in the sample; b) separating native or digested sample substances from each other; and c) analysing said substances by mass spectrometry. The invention also relates to a system for reducing total sample complexity in the above method, comprising a high capacity charge-selective column coupled to a MDLC work flow path comprising a cation exchange column and a RPC column. The system is followed by a MS/MS instrument.Type: ApplicationFiled: January 26, 2005Publication date: July 12, 2007Inventors: Johan Axelman, Anders Ljunglof
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Publication number: 20070125711Abstract: The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.Type: ApplicationFiled: November 1, 2004Publication date: June 7, 2007Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Anders Ljunglof, Jean-Luc Maloisel
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Publication number: 20070112178Abstract: The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.Type: ApplicationFiled: February 24, 2005Publication date: May 17, 2007Inventors: Bo-Lennart Johansson, Hans Johansson, Anders Ljunglof, Jean-Luc Maloisel, Nicolas Thevenin