Abstract: The present disclosure relates to recombinant sensor cells comprising an AND gate such that an expressible sequence (e.g., a reporter gene) is expressed after the occurrence of two separate triggering events. Nucleic acids, kits, and methods for making and using the recombinant sensor cells are also disclosed herein.

Abstract: Compositions, methods and uses of a recombinant virus and/or recombinant viral vector encoding a distinct antibody or antibody fragment generated from high-diversity nucleic acid library are presented. Preferably, the recombinant virus is genetically modified, low immunogenic virus, for example, an E2b-deleted adenovirus. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

Abstract: Compositions, methods and uses of high-diversity nucleic acid library that encodes a plurality of antibodies or antibody fragments are presented. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

Abstract: Compositions, methods, and uses of recombinant recombinant IL-8 antibody, fragment thereof or single chain variable fragment (scFv) having high affinity to IL-8 to target tumor-expressed or endogenous IL-8 are presented. Preferably, the recombinant IL-8 antibody or scFv fragment includes a VH segment comprising a first amino acid sequence selected from SEQ ID NO. 1-15, 31-32, and/or a VL segment comprising a second amino acid sequence selected from SEQ ID NO. 16-30, 33-34. The recombinant IL-8 antibody or scFv fragment can be formulated as pharmaceutical compositions to administer to a patient having a tumor to reduce metastasis of the tumor, reduce immune suppression in the tumor microenvironment or reduce Th2 mediated immune response.

Abstract: A system and method for managing website scripts includes operations which provide for user contributed website scripts for use by consumer users and computing systems providing services on their behalf. User contributed website scripts are used to periodically gather user data. Following receipt of a user contributed website script, validation operations are performed in order to ensure that the user contributed website script operates properly. Once an initial determination is made that the website script operates properly, award points are awarded to the contributing user, and contribution points are assigned. If performance of the user contributed website script fails after initial acceptance, the number of contribution points associated with the user contributed website script is reduced. If the number of contribution points associated with a user contributed website script falls below a predetermined threshold, the user contributed website script is rendered unavailable for use.

Abstract: A method of patterning a combined layer of an electrically-conductive film, such as indium-tin-oxide (ITO), that is disposed on a flexible substrate includes bending the combined layer about a radius of curvature. The combined layer is initially bent in a first direction so that the electrically-conducive film is distal to the radius of curvature, so as to form initial dielectric lines in the electrically-conductive film. The combined layer is then bent in another direction so that the electrically-conductive film is proximate to the radius of curvature to further enhance the dielectric performance of the initial dielectric lines. The dielectric lines electrically isolate a portion of the electrically-conductive film that is disposed therebetween, to form an electrically conductive electrode.