Abstract: The present invention relates to a method of conditioning a dry-packed chromatography column, which method comprises providing a column packed with inert and hydrophilic chromatography resin; and driving a solvent or solvent mix across the column. The solvent(s) are selected to be less hydrophilic than the chromatography resin; and the rate of the solvent flow is controlled to a rate which does not cause the system pressure to exceed a predefined threshold. The present invention is advantageously used in FLASH chromatography, where the generation of heat may pose problems e.g. to plastic frits or filters at high flow rates and/or wetting of dry resins.

Abstract: The present invention is a method for automated mass-directed separation of two or more components from a sample, which method comprises defining a solvent gradient by its changing composition; subjecting said solvent gradient to mass spectrometry (MS) to generate gradient signal(s); passing a gradient including at least part(s) of the defined gradient across a packed chromatography column to which a sample has been applied; subjecting the eluent exiting said column to MS to generate sample signal(s); generating a spectrum by subtracting the gradient signals from the sample signals across a selected range of m/z values; and directing a fraction collector to collect fraction(s) each comprising a separated component based on the spectrum generated.

Abstract: The present invention relates to a computer program, an apparatus and a method for correcting light absorption values measured for an eluate in connection with performing chromatography on a chemical sample, the sample being eluted with an eluent having an eluent profile with changing solvent composition during the elution of the sample.

Abstract: The present invention provides an apparatus that can be used to connect a flash chromatography instrument to a fraction collector and a mass detector to perform mass-directed flash chromatography.