Patents by Inventor Andras Guttman

Andras Guttman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210082676
    Abstract: Fluorescently labeled molecules of a sample are quantitated in an ion source device of a mass spectrometer. An illumination source device to illuminates at least a first portion of a sample. The sample is illuminated to excite fluorescently labeled molecules of the sample as it is being ionized in the ion source device. A two-dimensional digital image detector measures an image of at least a second portion of the illuminated first portion of the sample at each time interval of a plurality of time intervals. Each measured image at each of the plurality of time intervals is stored in a memory device. A trace of the intensity of the second portion as a function of time is calculated from the stored measured images. A quantity of the fluorescently labeled molecules is calculated from the trace of the calculated intensity of the second portion as a function of time.
    Type: Application
    Filed: May 23, 2019
    Publication date: March 18, 2021
    Inventors: Andras Guttman, Mate Szarka
  • Publication number: 20190299215
    Abstract: A holder that can be used to stand up a PCR tube on a flat surface or held in a user's hand. The holder has a first elongated portion, a second elongated portion and a third elongated portion. The first elongated portion having a magnet, the second elongated portion having a tube holding means and the third elongate portion acting as a stabilizer to allow the holder to standup in an upright position. The third elongate portion can also be used as a gripping surface to be held between fingers of a hand.
    Type: Application
    Filed: May 24, 2017
    Publication date: October 3, 2019
    Inventors: Andras Guttman, Marton Szigeti
  • Publication number: 20190234867
    Abstract: A separation device receives a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separated glycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration times of the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database of GU values for known glycan structures.
    Type: Application
    Filed: August 17, 2017
    Publication date: August 1, 2019
    Inventors: Gabor Jarvas, Marton Geza Szigeti, Andras Guttman, Jeff Chapman
  • Publication number: 20190227086
    Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol that can enable full automation and reduced sample preparation time relative to current methods of glycoanalysis. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method without requiring time-consuming sample preparation steps such as centrifugation or vacuum-centrifugation.
    Type: Application
    Filed: February 15, 2019
    Publication date: July 25, 2019
    Inventors: Andras Guttman, Clarence Lew, Marton Szigeti, Csaba Varadi
  • Patent number: 10247745
    Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol that can enable full automation and reduced sample preparation time relative to current methods of glycoanalysis. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method without requiring time-consuming sample preparation steps such as centrifugation or vacuum-centrifugation.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: April 2, 2019
    Assignee: Beckman Coulter, Inc.
    Inventors: Andras Guttman, Clarence Lew, Marton Szigeti, Csaba Varadi
  • Publication number: 20170175099
    Abstract: N-glycosylation profiling of glycoprotein biotherapeutics is an essential step in each phase of product development in the biopharmaceutical industry. For example, during clone selection hundreds of clones should be analyzed quickly from limited amounts of samples. On the other hand, identification of disease related glycosylation alterations can serve as early indicators for various pathological conditions in the biomedical field. We describe an improved packed bed column PNGase F functionalized column reactor. The reactor may be packed into a pipette tip column. In some embodiments, a second column or mixed stationary phase may be packed into the column to capture and purify the cleaved glycan prior to analysis. Complete N-glycan removal can be obtained in 10 minutes from all major N-linked glycoprotein types. The approach can be readily applied to automated sample preparation systems, such as liquid handling robots.
    Type: Application
    Filed: December 15, 2016
    Publication date: June 22, 2017
    Inventors: Marton Szigeti, Akos Szekrenyes, Zsolt Keresztessy, Andras Guttman
  • Publication number: 20170045538
    Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol that can enable full automation and reduced sample preparation time relative to current methods of glycoanalysis. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method without requiring time-consuming sample preparation steps such as centrifugation or vacuum-centrifugation.
    Type: Application
    Filed: April 27, 2015
    Publication date: February 16, 2017
    Inventors: Andras Guttman, Clarence Lew, Marton Szigeti, Csaba Varadi
  • Patent number: 8512959
    Abstract: A method or platform for monoclonal antibody based biomarker discovery is disclosed. The method according to the invention provides for the integration of analyte collection, hybridoma screening and nanovolume integrated mass spectrometry (NVIMS) to achieve a robust screening system that is capable, for example, of cutting 4-6 years off of the classical biomarker discovery and development process. The invention provides a platform for the rapid, high-throughput production, isolation and characterization of, e.g., disease specific biomarkers together with highly specific monoclonal antibodies. The method of the invention has a variety of applications such as, but not limited to, drug testing, biohazard applications, ecological applications, physiological applications and/or pathology screening applications. The method of the invention is also capable of being performed or used as or with a high-throughput screening process or system of the invention.
    Type: Grant
    Filed: February 9, 2005
    Date of Patent: August 20, 2013
    Assignee: Northwestern University
    Inventors: Laszlo Takacs, Andras Guttman, William S. Hancock, Barry L. Karger, Manuel Duval, Patrick Berna
  • Patent number: 8163152
    Abstract: A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, high speed, high throughput, carbohydrate analysis. In one aspect of the present invention, a high-performance capillary electrophoresis analyzer system has been optimized for carbohydrate analysis application. The system uses a multiplexed/time-staggered fluorescence type detection mechanism, with an integrated fiber optic array-based technology and a novel disposable gel-cartridge. Twelve carbohydrate samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available labeling agent such as APTS (representing 8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt) as an indicator, the capillary electrophoresis-based carbohydrate system/analyzer provides high resolving power in 2-15 minutes of separations.
    Type: Grant
    Filed: November 9, 2007
    Date of Patent: April 24, 2012
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Ming-Sun Liu, Andras Guttman
  • Publication number: 20110077164
    Abstract: The present invention relates to an efficient mAb panel-based expression profiling technology platform suitable for global and accurate measurement of proteins, peptides and metabolites in complex mixtures. The platform is comprised of new and well established technologies that are coupled in a unique fashion to provide a novel platform technology for (i) the discovery of differentially displayed elements of complex protein, peptide and metabolite mixtures and (ii) the development of robust mAb based assays that detect the differentially expressed elements.
    Type: Application
    Filed: September 24, 2010
    Publication date: March 31, 2011
    Inventors: Andras GUTTMAN, Laszlo Takacs
  • Patent number: 7816151
    Abstract: Reactors and methods for miniaturized reactions having enhanced reaction kinetics. In particular the subject matter is directed to chemical and biological reactions conducted in a nanoporous membrane environment. The subject matter contemplates methods for modifying the kinetics of reactions and devices for conducting reactions having modified kinetics. The subject matter also provides systems for rapid miniaturized reactions. Further the subject matter includes methods and kits for conducting a reaction with enhanced throughput and methods of conducting miniaturized, high throughput analyses of reaction products, and the like. Reactions performed on or within a nanoporous membrane exhibits improved kinetic characteristics.
    Type: Grant
    Filed: August 8, 2005
    Date of Patent: October 19, 2010
    Assignee: Syngenta Participations AG
    Inventors: Andras Guttman, Zsolt Ronai, Csaba Barta
  • Publication number: 20100227410
    Abstract: The present invention relates to methods for identifying antigens from complex analyte samples. More specifically, the invention relates to methods for identifying antigens recognized by monoclonal antibodies in a complex analyte sample. The methods of this invention use a first affinity chromatography via a “multiaffinity” step and a second, “singleaffinity” step that implements parallel affinity chromatography.
    Type: Application
    Filed: May 5, 2008
    Publication date: September 9, 2010
    Inventors: Laszlo Takacs, Janos Kadas, Andras Guttman
  • Publication number: 20090136966
    Abstract: The present invention relates to methods and compositions for the normalization of complex analyte mixtures. The invention allows the preparation of profiled samples from highly complex analyte mixtures, allowing the identification of relevant targets or biomarkers. The invention also relates to methods for producing devices, such as a support, suitable for normalization of complex analyte samples. The invention can be used for the normalization of any complex mixture, such as immunogenic libraries, particularly of human source, and to identify or produce biomarkers highly relevant to human traits or conditions.
    Type: Application
    Filed: July 27, 2006
    Publication date: May 28, 2009
    Applicant: BIOSYSTEMS INTERNATIONAL SAS
    Inventors: Laszlo Takacs, Andras Guttman, Mariana Kuras
  • Publication number: 20070293437
    Abstract: The present invention relates to an efficient mAb panel-based expression profiling technology platform suitable for global and accurate measurement of proteins, peptides and metabolites in complex mixtures. The platform is comprised of new and well established technologies that are coupled in a unique fashion to provide a novel platform technology for (i) the discovery of differentially displayed elements of complex protein, peptide and metabolite mixtures and (ii) the development of robust mAb based assays that detect the differentially expressed elements.
    Type: Application
    Filed: October 21, 2005
    Publication date: December 20, 2007
    Inventors: Andras Guttman, Laszlo Takacs
  • Publication number: 20060057576
    Abstract: The invention provides a microcapillay hybridization chamber made of a narrow bore tubing with probe segments. Each probe segment has oligonucleotide probes covalently attached to the inner wall of the tubing and the oligonucleotide probes within each segment have identical, known sequences. Many oligonucleotide probe segments can be present within a single centimeter of tubing. The invention further provides methods for using the microcapillary hybridization chambers in hybridization assays.
    Type: Application
    Filed: March 12, 2003
    Publication date: March 16, 2006
    Inventors: Jerzy Paszkowski, Andras Guttman, Xun Wang
  • Publication number: 20060051976
    Abstract: Reactors and methods for miniaturized reactions having enhanced reaction kinetics. In particular the subject matter is directed to chemical and biological reactions conducted in a nanoporous membrane environment. The subject matter contemplates methods for modifying the kinetics of reactions and devices for conducting reactions having modified kinetics. The subject matter also provides systems for rapid miniaturized reactions. Further the subject matter includes methods and kits for conducting a reaction with enhanced throughput and methods of conducting miniaturized, high throughput analyses of reaction products, and the like. Reactions performed on or within a nanoporous membrane exhibits improved kinetic characteristics.
    Type: Application
    Filed: August 8, 2005
    Publication date: March 9, 2006
    Inventors: Andras Guttman, Zsolt Ronai, Csaba Barta
  • Patent number: 6998251
    Abstract: Reactors and methods for miniaturized reactions having enhanced reaction kinetics. In particular the subject matter is directed to chemical and biological reactions conducted in a nanoporous membrane environment. The subject matter contemplates methods for modifying the kinetics of reactions and devices for conducting reactions having modified kinetics. The subject matter also provides systems for rapid miniaturized reactions. Further the subject matter includes methods and kits for conducting a reaction with enhanced throughput and methods of conducting miniaturized, high throughput analyses of reaction products, and the like. Reactions performed on or within a nanoporous membrane exhibits improved kinetic characteristics.
    Type: Grant
    Filed: January 14, 2002
    Date of Patent: February 14, 2006
    Assignee: Syngenta Participations AG
    Inventors: Andras Guttman, Zsolt Ronai, Csaba Barta
  • Publication number: 20040266021
    Abstract: A method of injecting, isolating and separating mixed analytes from one or more samples. The method includes collecting successive fractions from each of a plurality of samples at discrete points in time. Fractions may be analyzed at the time of collecting, or later, using one or more detector systems. In one embodiment, a processor controls the elutions of fractions by modulating the migration field in a separation pathway. The processor also controls distribution of the fraction into a particular collection well of a plurality of collection wells.
    Type: Application
    Filed: January 28, 2002
    Publication date: December 30, 2004
    Inventors: Andras Guttman, Liang Shi, Xun Wang
  • Publication number: 20030135030
    Abstract: Reactors and methods for miniaturized reactions having enhanced reaction kinetics. In particular the subject matter is directed to chemical and biological reactions conducted in a nanoporous membrane environment. The subject matter contemplates methods for modifying the kinetics of reactions and devices for conducting reactions having modified kinetics. The subject matter also provides systems for rapid miniaturized reactions. Further the subject matter includes methods and kits for conducting a reaction with enhanced throughput and methods of conducting miniaturized, high throughput analyses of reaction products, and the like. Reactions performed on or within a nanoporous membrane exhibits improved kinetic characteristics.
    Type: Application
    Filed: January 14, 2002
    Publication date: July 17, 2003
    Inventors: Andras Guttman, Zsolt Ronai, Csaba Barta
  • Publication number: 20030132117
    Abstract: High throughput electrophoresis having a plurality of samples migrating through a highly resistive separatory media under an electric field. Multiple electrophoresis runs are conducted using a single separatory media. Automated methods of data analysis yields quantitative and qualitative data using optical densitometer or other detection systems. Controlled operating temperatures and small geometry reduces dispersion inaccuracy in banded data.
    Type: Application
    Filed: January 14, 2002
    Publication date: July 17, 2003
    Inventors: Andras Guttman, Bart Wanders, Phil Alei