Abstract: The present disclosure provides methods to diagnose and/or treat hemoglobinopathies via comparative analysis of cell magnetic properties.

Abstract: A stimulation system for a patient is provided. The system comprises: at least one implantable device comprising at least one implantable antenna; and an external device comprising at least one external antenna, wherein the at least one external antenna transfers power to the at least one implantable antenna. The at least one implantable device delivers therapy to the patient. A patient attachment device or body covering positions the at least one external antenna relative to the patient.

Abstract: Disclosed are method of using compositions comprising PolyHSA to preventing, protecting and/or treating conditions such as endothelial dysfunction, and hypercytokinemia.

Abstract: Tangential flow filtration (TFF) is a size-based separation method conventionally used for buffer exchange, concentration, pathogen removal, and for coarse purification. Disclosed herein, TFF was used for selective purification of proteins in their complexed form. In some examples, this process was demonstrated to recover human serum albumin (HSA) in its complexed form from an artificially produced mixture of hemoglobin (Hb) and HSA and from plasma using an anti-HSA polyclonal immunoglobulin G (IgG) as the target-protein binding molecule (TPBM). Moreover, another embodiment of the method recovered haptoglobin (Hp) in its complexed form from human Cohn Fraction IV using Hb as the TPBM. In addition, a mathematical model used to describe the TFF purification process provided that product recovery could be increased without loss of purity by introducing TFF filters with the same MWCO in series.

Abstract: The present disclosure provides methods of treating plasma protein imbalances or depletion (e.g., plasma protein imbalances or depletion caused by hemorrhagic shock or other clinical conditions), in particular by administering protein compositions derived from plasma or plasma isolates.

Abstract: Methods for purifying and isolating extracellular vesicles (EVs) from a biofluid using a sequential processing. Tangential flow filtration is applied to the biofluid to increase the concentration of EVs in the biofluid. After this is achieved, enrichment mode is halted and the biofluid is processed in diafiltration mode to remove contaminants (up to 99.9%). After performing the tangential flow filtration step, the concentration of EVs in the biofluid is further increased by ultracentrifugal filtration. After performing the ultracentrifugal filtration step, EVs of a particular target type are separated from other EVs by immunomagnetic affinity separation. In some implementations, the methods are used to isolate and quantify tumor EVs for cancer evaluation. Additionally, these methods can be used with a scaling factor to quantify EVs from a less concentrated biofluid such as, for example, urine.

Abstract: Disclosed herein are oxygen transporting formulations, in particular those composed of hemoglobin-based nanoparticles, and their use and process of manufacture. These formulations are more uniform and monodisperse than prior hemoglobin-based oxygen carriers, such as polymeric hemoglobin. In addition, these formulations provide higher hemoglobin encapsulation efficiencies and higher hemoglobin content that hemoglobin-containing vesicles.

Abstract: Provided herein are apohemoglobin-haptoglobin complexes as well as apohemoglobin-haptoglobin complexes comprising an active agent coordinated thereto. Methods of using these compositions are also described.

Abstract: Provided herein are methods for purifying proteins from mixtures of proteins using ultrafiltration, such as tangential-flow filtration (TFF). Provided herein are methods for isolating an apoprotein from a protein solution comprising a conjugated protein, wherein the conjugated protein comprises the apoprotein and a hydrophobic ligand associated with the apoprotein.