Abstract: This invention relates to a supplement, the use thereof and method for supplementing the concentration of free cellular L-proline in an organism, for restricting dehydration of such an organism, the supplement comprising an effective amount of free L-proline. The supplement is effective in reducing the levels of free radicals in organisms experiencing water stress. This invention further relates to a method of treating dehydration in humans and animals including the step of administering to an individual in need thereof an effective amount of a supplement according to the third aspect of the invention. An effective amount of free L-proline is typically between 20 and 1000 mg, preferably 100 mg free L-proline per kilogram body mass of the organism, three times a day.

Abstract: This invention relates to the use of free L-proline in the preparation of a supplement, and to a supplement so prepared, as well as a method using such a supplement for supplementing the concentration of cellular free L-proline in abalone and for restricting the dehydration of abalone. The supplement is further effective in reducing the levels of free radicals in abalone experiencing water stress and hypoxic stress. The invention further relates to a method of restricting the dehydration of abalone during storage and transportation including the step of administering to abalone an effective amount of free L-proline.