Abstract: The present invention provides the use of the antigens CD49e and/or CD49f as selection markers for enrichment, isolation, detection and/or analysis of atrial and ventricular cardiomyocytes and a method for enrichment, isolation, detection and/or analysis of these cells from a sample comprising cardiomyocytes. In addition substantially pure compositions of these cardiomyocyte subpopulations are provided.

Abstract: The invention is directed to a Perfusion device for biological tissues comprising a casing having two parts, a first part (1) and a second part (9), a holder (7) for a plurality of hollow penetration structures (8), wherein the hollow penetration structures (8) are provided with at least one orifice having fluid communication through the holder (7) a support (5) for the biological tissue (6) characterized in that the support (5) for the biological tissue (6) is positioned in the casing at a distance to the holder (7) that by joining the first part (1) and the second part (9) to form the casing, the hollow penetration structures (8) are in proximity to the holder (7). Use of the perfusion device in a process for disaggregation of a biological tissue to yield target cells.

Abstract: The present invention provides a method for analyzing levels of markers of subpopulations of vesicles in a sample comprising a heterogeneous population of said vesicles, the method comprising a) contacting said sample with a set of at least two capture agents, marker of said vesicles; thereby capturing at least two subpopulations of said vesicles, b) contacting the captured at least two subpopulations of vesicles with at least two different detection agents, thereby labeling at least two markers of said at least two subpopulations of said vesicles inversely with said at least two capture agents and at least two detection agents, thereby generating an at least 2×2 matrix of signal intensities, and c) determining the levels of said at least two markers of said at least two subpopulations of said vesicles, wherein said levels of said at least two markers of subpopulations of said vesicles are determined relative to each other.

Abstract: Object of the invention is a process for depleting host cells from a xenograft of human cells on a murine host characterized in a) fragmenting the xenograft into a sample comprising a single cell suspension b) subjecting the sample to antibodies specific for a murine CD9 epitope coupled to a detection means c) depleting the cell suspension from cells bound by the CD9-antibodies using the detection means d) collecting the cells not bound by the CD9-antibodies as target cells.

Abstract: The present invention provides the use of the biomarkers SSEA4 and/or ST3GAL2 for assessing the outcome for chemotherapeutic treatment of a cancer in an individual and methods thereof.

Abstract: The invention relates to a cell containing a gene encoding a conditional transgenic surface marker that is detectable upon expression on the surface of the cell, wherein the gene encoding a conditional transgenic surface marker comprises: (i) a promoter, operably linked to (ii) a first transcription sequence, and (iii) a second transcription sequence encoding the surface marker, whereby the first transcription sequence prevents the transcription of the second transcription sequence, whereby the first transcription sequence is conditionally removable such that the second transcription sequence is transcribable, and whereby the surface marker renders the cell sortable through the detection of the conditional transgenic surface marker. Furthermore, the invention relates to a construct for generating such a cell, and to a method for separating such a cell from a population of cells.

Abstract: The present invention provides the use of the cell surface antigen CD51 as a negative selection marker for neuronal cells and a method for enrichment, isolation and/or detection of neuronal cells comprising the steps a) contacting a sample containing neuronal cells with an antigen-binding fragment specific for the CD51 antigen coupled to a solid phase, thereby labeling the CD51 positive cells of said sample and b) isolating the non-labeled cells of said sample.

Abstract: The present invention provides the use of the antigens CD49e and/or CD49f as selection markers for enrichment, isolation, detection and/or analysis of atrial and ventricular cardiomyocytes and a method for enrichment, isolation, detection and/or analysis of these cells from a sample comprising cardiomyocytes. In addition substantially pure compositions of these cardiomyocyte subpopulations are provided.

Abstract: A cell strainer for separating particles from a cell suspension, having an upper portion with at least one filter area lying essentially perpendicular to the direction of flow of the suspension and a lower portion adapted to fit into the openings of at least two sample tubes with different sized openings. The lower portion has a first section with shoulders or flanges having the diameter of the opening of a first tube and a second section having an inner and outer wall serving as a receptacle for the neck of a second tube, where the diameter of the first section is larger than the diameter of the second section.

Abstract: The invention relates to a cell containing a gene encoding a conditional transgenic surface marker that is detectable upon expression on the surface of the cell, wherein the gene encoding a conditional transgenic surface marker comprises: (i) a promoter, operably linked to (ii) a first transcription sequence, and (iii) a second transcription sequence encoding the surface marker, whereby the first transcription sequence prevents the transcription of the second transcription sequence, whereby the first transcription sequence is conditionally removable such that the second transcription sequence is transcribable, and whereby the surface marker renders the cell sortable through the detection of the conditional transgenic surface marker. Furthermore, the invention relates to a construct for generating such a cell, and to a method for separating such a cell from a population of cells.

Abstract: A receiving device for object holders comprises a base part. A plurality of centering elements are connected with the base part. The centering elements are arranged such that a plurality of receiving areas for receiving one object holder each are configured. The centering elements form a preferably circumferential centering frame around each receiving area.

Abstract: A device for carrying out chemical and/or biological reactions comprises a sample chamber (38) formed by a first portion (12) and a second portion (26) and designed for holding the sample. With the aid of a moving means (46,50) the sample can be moved in the sample chamber (38). The second portion (26) is connected via an intermediate element (27) with a heating and cooling-control element (29) for heating and cooling the sample. For ensuring good temperature conductivity and resistance to the sample of the second portion (26), in a preferred embodiment, the second portion (26) is made of ceramic.

Abstract: An analyzing device for analyzing chemical and/or biological samples comprises a plurality of base parts, e. g. object holders, in a receiving device. The base parts are closed by a lid which is configured such that each base part and the lid form a test chamber for receiving the sample. Further, a conveying means for moving the sample in the test chamber is provided. The pumps of the conveying means are connected via channels with the test chamber and are controlled such that a portion of the sample liquid is alternately drawn off the test chamber and supplied back thereto by the pumps.

Abstract: A nucleic acid complex with an anchor oligonucleotide, an arbitrary nucleic acid or an arbitrary oligonucleotide and a linker nucleic acid, wherein the linker nucleic acid is partially hybridized with the anchor nucleotide and partially hybridized with the arbitrary nucleic acid or the arbitrary oligonucleotide and the anchor nucleotide is immobilized on a support by its 3?-terminus.

Abstract: A receiving device for object holders comprises a base part. A plurality of centering elements are connected with the base part. The centering elements are arranged such that a plurality of receiving areas for receiving one object holder each are configured. The centering elements form a preferably circumferential centering frame around each receiving area.

Abstract: The invention relates to a support. Oligonucleotides or polynucleotides are covalently bound with the 5′- or 3′-termination on least one main surface of said support via bifunctional spacers and bifunctional linkers.

Abstract: An analyzing device for analyzing chemical and/or biological samples comprises a plurality of base parts (12) provided in a receiving device (10), for example object supports. Said base parts are sealed by means of a head part (26,80), which is configured in such a way that each base part (12) and the head part (26,80) form a test chamber (38) for receiving the samples. The analyzing device also comprises a foot part (58,88) for receiving the base part (12). The support (58,88) and the head part (26,80) are displaceable in relation to each other in order to seal the test chamber (38). The head piece (26,80) and/or the foot part (58,88) are arranged in such a way that the base part is oriented relative to the head part (56) when the test chamber is sealed. As a result, deterioration of the base parts (12) is prevented when the test chamber (38) is closed.

Abstract: An analyzing device for analyzing chemical and/or biological samples comprises a plurality of base parts (12), e.g. object holders, in a receiving device (10). Said base parts (12) are closed by a lid (26) which is configured such that each base part (12) and the lid (26) form a test chamber (38) for receiving the sample. Further, a conveying means (46,50;68) for moving the sample in the test chamber (38) is provided. The pumps (46,50) of the conveying means are connected via channels (40,42) with the test chamber and are controlled such that a portion of the sample liquid is alternately drawn off the test chamber (38) and supplied back thereto by the pumps (46,50).

Abstract: A nucleic acid complex with an anchor oligonucleotide, an arbitrary nucleic acid or an arbitrary oligonucleotide and a linker nucleic acid, wherein the linker nucleic acid is partially hybridized with the anchor nucleotide and partially hybridized with the arbitrary nucleic acid or the arbitrary oligonucleotide and the anchor nucleotide is immobilized on a support by its 3′-terminus.

Abstract: A receiving device for object holders (12) comprises a base part (14). A plurality of centering elements (16,18,20) are connected with the base part (14). Said centering elements (16,18,20) are arranged such that a plurality of receiving areas (22) for receiving one object holder (12) each are configured. The centering elements (16,18,20) form a preferably circumferential centering frame around each receiving area (22).