Abstract: The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.

Abstract: The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.

Abstract: The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.

Abstract: The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.

Abstract: The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.

Abstract: The design of new ribozymes capable of self-catalyzed trans-splicing which are base a upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.

Abstract: In a process for purifying and deaerating the feed water line in the circulation system of a power-generating plant heated by nuclear power, the following steps are carried out in order to solve acute problems of steam-generator corrosion:(a) when the preheater train is filled, with the steam-generator isolating valve (40) closed, the condensate flows several times via a recirculation line (37, 52, 53) back to the condensate-collecting vessel (6) and to the condensate purification apparatus (9);(b) below the water level (7) in the condensate-collecting vessel (6), a deaerating medium (37,38,39) is introduced into the condensate via spray nozzles, rises in the condensate and forms in the deaerating chamber (44), as result of displacement of the air present in its, a cushion which is conducive to oxygen deaerating;(c) the steam produced during deaerating is precipitated in an additional condenser (48) which is located in the venting line upstream of the vacuum pump (47) and which is subjected to a secondary coo

Abstract: In order to improve upon the prior arrangement of a valve within a liquid distributor head for a mixing pre-heater degasifier it is proposed that a valve containing a valve plate, valve shaft, valve spring and spring plate, is arranged within the mixing pre-heater centrally within a deflection screen or baffle and at the same time the valve is surrounded by a protective housing in order to prevent that, in the event of possible rupture of the valve spring any broken pieces thereof will drop into the mixing pre-heater.