Patents by Inventor Andrea Candelli
Andrea Candelli has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20230384307Abstract: The current invention relates to an improved method for detecting and/or measuring interaction between lymphocytes and target cells. In particular the current invention relates to a method for improved differentiation between lymphocytes and target cells that have formed (mature) immunological synapses and lymphocytes and target cells that have not, or only to a limited extend, formed such immunological synapses. The current invention can be used to discriminate between lymphocytes and target cells that specifically interact with each other, for example when the lymphocyte specifically interacts with an antigen presented on the target cell, and those that do not or only to a limited extend.Type: ApplicationFiled: October 20, 2021Publication date: November 30, 2023Inventors: Andrea CANDELLI, Keith Alan BAILEY
-
Publication number: 20230349895Abstract: A method for collecting one or more cellular bodies in dependence on their binding force with a sample surface is disclosed. The method comprises providing a sample holder comprising a holding space. The holding space comprises, e.g. is substantially filled with, a fluid medium and comprises the sample surface and the one or more cellular bodies in the fluid medium. Further, the one or more cellular bodies are contacting said sample surface. The method also comprises rotating the sample holder around an axis of rotation for causing a centrifugal force on the one or more cellular bodies herewith separating the one or more cellular bodies from the sample surface. The method also comprises collecting the separated one or more cellular bodies. Also, a method for detection cellular bodies is described. This disclosure further relates to corresponding systems, computer-implemented methods, computer programs and computer-readable storage media having stored such computer programs.Type: ApplicationFiled: August 26, 2021Publication date: November 2, 2023Inventors: Mattijs De Groot, Andrea Candelli, Gerrit Sitters, Olivier Theodoor Heyning
-
Publication number: 20220118453Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.Type: ApplicationFiled: November 18, 2021Publication date: April 21, 2022Applicant: LUMICKS CA HOLDING B.V.Inventors: Andrea Candelli, Felix Oswald, Gerrit Sitters
-
Patent number: 11207684Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.Type: GrantFiled: November 2, 2017Date of Patent: December 28, 2021Inventors: Andrea Candelli, Felix Oswald, Gerrit Sitters
-
Publication number: 20190255527Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.Type: ApplicationFiled: November 2, 2017Publication date: August 22, 2019Applicant: LUMICKS TECHNOLOGIES B.V.Inventors: Andrea Candelli, Felix Oswald, Gerrit Sitters
-
Patent number: 9952421Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1,OT2,OT3). In one method, trapping beams (TB1,TB2,TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1,OT2,OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (?A) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (?A) of the detector beam (AB) is measured.Type: GrantFiled: February 4, 2016Date of Patent: April 24, 2018Assignee: Stichting VUInventors: Andreas Sebastian Biebricher, Andrea Candelli, Iddo Heller, Niels Laurens, Erwin Johannes Gerard Peterman, Gijs Jan Lodewijk Wuite
-
Publication number: 20180024342Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1, OT2, OT3). In one method, trapping beams (TB1, TB2, TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1, OT2, OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (?A) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (?A) of the detector beam (AB) is measured.Type: ApplicationFiled: February 4, 2016Publication date: January 25, 2018Applicant: Stichting VuInventors: Andreas Sebastian Biebricher, Andrea Candelli, Iddo Heller, Niels Laurens, Erwin Johannes Gerard Peterman, Gijs Jan Lodewijk Wuite
-
Patent number: 9766180Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).Type: GrantFiled: June 2, 2014Date of Patent: September 19, 2017Assignee: Stichting VU-VUmcInventors: Gijs Jan Lodewijk Wuite, Erwin Johannes Gerard Peterman, Iddo Heller, Gerrit Sitters, Andrea Candelli, Stefan Walter Hell
-
Publication number: 20160139050Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).Type: ApplicationFiled: June 2, 2014Publication date: May 19, 2016Inventors: Gijs Jan Lodewijk Wuite, Erwin Johannes Gerard Peterman, Iddo Heller, Gerrit Sitters, Andrea Candelli, Stefan Walter Hell