Abstract: Multi-well plate compatible device or insert for three-dimensional (3D) cell cultures, use and kit thereof are provided. The device includes outer and inner walls. the inner wall defining a volume and is located within the outer wall, forming a cavity therebetween. It also includes a base configured to position a cell culture sample below the volume within the inner wall and one or more partitions connected to the base. connecting the inner wall to the outer wall and segmenting the cavity into a plurality of voids. Openings in surfaces forming the voids allow fluid flow from a first void. below the volume within the inner wall to a second void, the fluid flow configured to interact with the cell culture sample when flowing through a sample region within the inner wall. The sample region has a depth defined by a height of the inner wall greater than a length defined by an inner distance across the volume within the inner wall.