Abstract: The present invention refers to a device for measuring impedance in organotypic tissue comprising at least one recording chamber with a liquid permeable membrane supporting the organotypic tissue, at least one bottom electrode and at least one top electrode, wherein the liquid permeable membrane divides the recording chamber into a top chamber and a bottom chamber, wherein at least the bottom chamber contains culture medium for the organotypic tissue, and the bottom electrode(s) is/are located in the bottom chamber and the top electrode(s) is/are located in the top chamber, and wherein the organotypic tissue is located between the bottom electrode(s) and the top electrode(s). The present invention also refers to the use of the device according to the present invention for measuring impedance in organotypic tissue.

Abstract: The present invention refers to a device for measuring impedance in organotypic tissue comprising at least one recording chamber with a liquid permeable membrane supporting the organotypic tissue, at least one bottom electrode and at least one top electrode, wherein the liquid permeable membrane divides the recording chamber into a top chamber and a bottom chamber, wherein at least the bottom chamber contains culture medium for the organotypic tissue, and the bottom electrode(s) is/are located in the bottom chamber and the top electrode(s) is/are located in the top chamber, and wherein the organotypic tissue is located between the bottom electrode(s) and the top electrode(s). The present invention also refers to the use of the device according to the present invention for measuring impedance in organotypic tissue.

Abstract: The present invention relates to a device and a method for parallel recording of impedance spectra and field potential of cells in vitro.

Abstract: Disclosed herein are methods for identifying an agent for treating or preventing neurogenerative disease and to methods for recapitulating tauopathies using a tau protein that includes at least four different mutations that cause the condition frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), and to nucleic acids encoding the tau protein.

Abstract: In a method for label-free detection and classification of cellular alterations, cells, cell-spheroids or tissue samples are introduced in culture reservoirs of a cultivation chamber plate. The cells, cell-spheroids or tissue sample are positioned in the microcavities of the cultivation chamber plate by movement of a rotary shaker that is provided with an amplifier board and mounting device on which the cultivation chamber plate is mounted. The impedance of the cells, cell-spheroids or tissue samples between two microelectrodes and/or electrogenic activity of the cells, cell-spheroids or tissue sample on every microelectrode and their changes during the cultivation and between different culture reservoirs are determined.

Abstract: The present invention relates to a device and a method for parallel recording of impedance spectra and field potential of cells in vitro.

Abstract: The present invention relates to a tau protein comprising at least four different mutations selected from group consisting of mutations which cause the condition frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), and to nucleic acids encoding said tau protein. The invention further pertains to methods for identifying an agent for treating or preventing neurogenerative disease, and to methods for recapitulating tauopathies.

Abstract: The invention relates to an integrated cultivation and measurement device for label-free detection and classification of cellular alterations, in particular for generation and characterisation of cell-spheroids and monitoring the condition of the cell-spheroids in real time, comprising a) a mounting device for a cultivation chamber plate, b) an amplifier board linked with the contacts for the microelectrodes in the mounting device, c) a rotary shaker, on which the amplifier board and the mounting device for the cultivation chamber plate are placed, and d) a control unit, that is linked with the amplifier board and the rotary shaker, wherein the control unit allows recording, analyzing of data and controlling of the movement of the rotary shaker. The cultivation chamber plate has several culture reservoirs, wherein the bottom of each culture reservoir forms a microcavity and each microcavity features microelectrodes on the microcavity walls. The mounting device has contacts for the microelectrodes.

Abstract: Disclosed is a device for detecting bioelectric signals from spheroids comprising a measuring chamber having a measuring chamber wall which encloses a volume, which is open at least at one side, is composed of an electrically non-conducting material, and has, in at least one measuring region, an inner cross section, which corresponds as far as possible to the largest cross section of a spheroid, comprising at least a number of electrodes which are disposed in a common plane inside said measuring chamber wall and each electrode has a freely accessible electrode surface which is oriented towards the measuring region, and comprising an impedance measuring arrangement which is connected to the electrodes. The device and the method can be used to test substances in 3D biological in-vitro (three-dimensional) cell aggregates.

Abstract: The present invention refers to a device for measuring impedance in organotypic tissue comprising at least one recording chamber with a liquid permeable membrane supporting the organotypic tissue, at least one bottom electrode and at least one top electrode, wherein the liquid permeable membrane divides the recording chamber into a top chamber and a bottom chamber, wherein at least the bottom chamber contains culture medium for the organotypic tissue, and the bottom electrode(s) is/are located in the bottom chamber and the top electrode(s) is/are located in the top chamber, and wherein the organotypic tissue is located between the bottom electrode(s) and the top electrode(s). The present invention also refers to the use of the device according to the present invention for measuring impedance in organotypic tissue.

Abstract: The present invention relates to a device and a method for characterizing spheroids. The to-be-characterized spheroid is introduced into a tube of an electrically insulating material and with an inner diameter which is smaller than the diameter of the to-be-characterized spheroid in such a manner that the latter is in mechanical contact over the entire circumference with the inner wall of the electrically insulated tube. Electrodes are disposed on both sides of the spheroid in the tube. By means of these electrodes, a current flow can be generated through the spheroid and the resulting drop in voltage at the spheroid can be measured. The invented arrangement and the corresponding method permits producing an impedance spectrum of spheroids with high sensitivity, thereby realizing rapid and nondestructive characterization of spheroids.