Abstract: A method to express myoglobin in a fungus is described. The method includes optimizing a codon for a fungal host (e.g., Trichoderma reesei), inserting the optimized codon into a plasmid (e.g., a pTrEno plasmid or one of its derivatives), and ultimately collecting the secreted myoglobin from a feeding media in response to the myoglobin being expressed extracellularly. Optionally, the myoglobin is purified.

Abstract: Methods for creating edible scaffolding using algae, and more specifically, a protocol for the sterilization and decellularization of Durvillaea antarctica, Chilean seaweed, or cochayuyo to prepare the cochayuyo to be suitable for the culture of cells are described herein. The scaffold may be used for cell growth and is composed of sterile decellularized cochayuyo rehydrated with adhesion/carrier proteins. The decellularized algae supports cell growth, differentiation, and nutrient diffusion due to its porosity. The product is completely edible. The methods allow for the seaweed material to be dried and rehydrated without problems before and/or after decellularization. Also, the sample described herein can be easily sterilized without losing its form and can be hydrated with proteins. Additionally, the scaffolding can be used as a microcarrier because it can float inside the cell media, and one wouldn't need to change the cells for differentiation.