Abstract: The present invention relates to a method for controlling process parameters in a bioreactor when producing a target product with a pre-determined critical to quality, CTQ, profile. The method comprises: monitoring (S20) at least one product quality attribute, PQA, for the target product; identifying (S30) trends and/or deviations by comparing the monitored at least one PQA to the CTQ profile of the target product; and controlling (S40) process parameters of the bioreactor based on the identified trends and/or deviations to maintain the target product within the CTQ profile.

Abstract: The present invention relates to a computer implemented method (400) performed by a controller (C) configured to control a bioprocess comprised in a bioreactor (BR), the method comprising obtaining (410) measurement results by performing spectrophotometry of a bioprocessing fluid (FL), generating (420) control parameters based on the measurement results and one model and, controlling (430) the bioprocess using the generated parameters.

Abstract: The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

Abstract: A bio-processing system (100) is presented. The bio-processing system (100) includes one or more bio-processing units (110-114), one or more process supporting devices (116, 118), one or more sensors (120-132), and a bio-processing workflow controller (104) wirelessly coupled to at least one of the one or more bio-processing units (110-114), the one or more process supporting devices (116, 118), and the one or more sensors (120-132). The bio-processing workflow controller (104) includes a processor (202) configured to create a reconfigurable bio-processing workflow, and where the reconfigurable bio-processing workflow is representative of an arrangement of one or more of the one or more bio-processing units (110-114), the one or more process supporting devices (116, 118), and the one or more sensors (120-32) to perform at least one bio-processing operation.

Abstract: A bio-processing system (100) for wirelessly powering one or more sensors (116-128, 400) is presented. The system (100) includes bio-processing units (106-110), process supporting devices (112-114), energy sources (146-148), and sensors (116-128, 400) including an energy harvesting unit (402) and an energy storage unit (404). The system (100) includes a power management subsystem (104, 200) wirelessly coupled to the sensors (116-128, 400) and including a processor (202) configured to wirelessly monitor energy consumption of the sensors (116-128, 400) and a level of energy stored in corresponding energy storage units (404), select at least one sensor (116-128, 400) based on the energy consumption of the sensors (116-128, 400) and corresponding levels of energy stored in the energy storage units (404), and identify at least one active energy source (146-148) as a power source, where the identified power source is configured to wirelessly transfer power to the selected sensor (116-128, 400).

Abstract: The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

Abstract: The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.

Abstract: The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

Abstract: The present invention relates to a method and a device for small scale reactions, such as sample preparation of a desired substance in a sample. In the method using the device samples mixed with functionalized magnetic particles are magnetically transferred between different working stations on the device. The method uses a hydrophobic surface, such as a Petri dish, provided with hydrophilic spots of, for example, agarose beads located on said hydrophobic surface and provided with buffers, reactants or ligands.

Abstract: The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.

Abstract: The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.

Abstract: The present invention relates to a separation media and a method for separation of cells from different cell sources, such as umbilical cord blood, using the separation media to obtain the desired cells, such as stem cells. The separation media has beads with a diameter of 200-500 ?m and is provided with cell separation ligands. The cell specific ligands are preferably CD3 and CD19 for depletion of B and T cells and production of a stem cell rich product.

Abstract: The present invention relates to separation matrices comprising base matrices with first ligands comprising hydrophobic functions covalently bound to said base matrices and with extenders covalently bound to said base matrices, said extenders comprising second ion exchange ligands.

Abstract: The present invention relates to a method for cell expansion. In the method, preferably a cell culture product is used, such as a microcarrier, or other adherent cell culture surface, comprising degradable polysaccharide, preferably starch, modified with small molecular weight cell-binding ligands. This allows recovery (detachment) of adhered cells to be aided by degradation of the culture surface with enzymatic agents, such as amylase. The method for cell expansion comprises the following steps: a) adding cells, culture medium and cell culture surface comprising a degradable polysaccharide with guanidine group containing ligands to a bioreactor; b) expanding said cells by adherent cell culture; and c) aiding the detachment of said cells by exposing them to a polysaccharidase to degrade the culturing surface.

Abstract: The present invention relates to a method for generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of (a) providing at least one scaffold defined by the general formula (I): H2N—(X1)n—S—S—(X2)m—CH2—NH2 wherein X1 and X2 irrespective of each other are carbon atoms or heteroatoms, and n and m irrespective of each other are integers of 1 to 5; (b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities of at least one arm are protected, on each scaffold by derivatisation of the nucleophilic NH2 groups of the scaffold; (c) reducing the disulfide bond of the derivatised scaffold; and, if required (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment, the reduction of the disulfide bond and the deprotection step is performed essentially simultaneously.

Abstract: The present invention relates to magnetic beads suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The magnetic beads are composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and these are then enclosed in a hydrophilic porous polymer, preferably agarose. This provides porous biocompatible beads without metal leakage. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer is preferably provided with ligands having affinity for selected biomolecules.

Abstract: The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

Abstract: The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.

Abstract: The present invention relates to a separation media and a method for separation of cells from different cell sources, such as umbilical cord blood, using the separation media to obtain the desired cells, such as stem cells. The separation media has beads with a diameter of 200-500 ?m and is provided with cell separation ligands. The cell specific ligands are preferably CD3 and CD19 for depletion of B and T cells and production of a stem cell rich product.

Abstract: The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.