Patents by Inventor Andreas Castan
Andreas Castan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11927503Abstract: The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.Type: GrantFiled: September 9, 2019Date of Patent: March 12, 2024Assignee: Cytiva Sweden ABInventors: Sameera Simha Hindupur, Manoj Kumar Ramakrishna, Andreas Castan, Swapnil Puranik, Klaus Gebauer
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Publication number: 20240043879Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.Type: ApplicationFiled: September 14, 2023Publication date: February 8, 2024Inventors: Daniel Ivansson, Andreas Castan
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Publication number: 20240018460Abstract: Methods and apparatus for scaling in bioprocess systems are disclosed. An example apparatus for bioprocess scaling includes at least one memory to store instructions, and processor circuitry to execute the instructions to identify an operating parameter of a target bioreactor, determine an upper boundary or a lower boundary defining a design space for at least one bioreactor process parameter to match at least one of a first target parameter range or a second target parameter range based on the operating parameter, simulate changes in the first target parameter range or a second target parameter range based on an adjustment to the upper boundary or the lower boundary in the design space, and configure the target bioreactor using output obtained from the adjustment to the upper boundary or the lower boundary to identify a match between the first target parameter range or the second target parameter range.Type: ApplicationFiled: September 29, 2021Publication date: January 18, 2024Inventors: Andreas CASTAN, Nagaraju KONDURU, Ashish HANDA, Neelima BODDAPATI, Helena ÖHRVIK, Alok Singh CHAUHAN
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Patent number: 11802297Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.Type: GrantFiled: February 23, 2018Date of Patent: October 31, 2023Assignee: Cytiva Sweden ABInventors: Daniel Ivansson, Andreas Castan
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Publication number: 20230093940Abstract: The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.Type: ApplicationFiled: December 6, 2022Publication date: March 30, 2023Inventors: Andreas Castan, Klaus Gebauer
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Patent number: 11549091Abstract: The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.Type: GrantFiled: August 27, 2013Date of Patent: January 10, 2023Assignee: Cytiva Sweden ABInventors: Andreas Castan, Klaus Gebauer
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Patent number: 11525111Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.Type: GrantFiled: September 27, 2019Date of Patent: December 13, 2022Assignee: Cytiva Sweden ABInventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
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Publication number: 20220242903Abstract: The present disclosure is directed to method (100) for separating a biomolecule from a fluid (2), wherein the biomolecule is produced by biological cells in a perfusion bioreactor (3), the method comprising: forwarding (110) a product fluid comprising the biomolecule and biological cells from the perfusion bioreactor (3) during a bioreactor product output time period; loading (210) a first separation device (4) with the product fluid obtained in step (110) during a first separation loading time period, which constitutes a first part of a first separation cycle time period; pausing (130) the forwarding of the product fluid from the perfusion bioreactor during a bioreactor non-product output time period; and pausing (220) the loading of the first separation device (4) during a second part of the first separation cycle time period.Type: ApplicationFiled: June 11, 2020Publication date: August 4, 2022Inventors: Niklas JUNGNELIUS, Andreas CASTAN
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Publication number: 20220234012Abstract: A sparger assembly for a bioprocessing system includes a first layer having a plurality of pores of a first size, and a second layer disposed above the first layer and having a plurality of holes of a second size, the second size being greater than the first size. The pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.Type: ApplicationFiled: May 21, 2020Publication date: July 28, 2022Applicant: GLOBAL LIFE SCIENCES SOLUTIONS USA LLCInventors: ANDREAS CASTAN, RICHARD DAMREN
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Publication number: 20220127557Abstract: A bioreactor system for culturing cells, comprising: —at least one bioreactor (3); and —at least one hydrocyclone (5a; 5b; 5c; 5d, 5d?; 5e) comprising: —a cell culture inlet (7) which is connected to a cell culture outlet (9) of the bioreactor (3) via a pump (11), whereby a cell culture from the bioreactor can be transferred to the hydrocyclone (5a: 5b; 5c; 5d, 5d?; 5e) when a cell culture is provided in the bioreactor, —a hydrocyclone separation part (13) into which cell culture can be introduced from the cell culture inlet (7), —a top outlet (15) through which a part of the cell culture with decreased cell concentration can be transferred after separation in the hydrocyclone separation pan (13); and —a bottom outlet (17) through which a pan of the cell culture with increased cell concentration can be transferred after separation in the hydrocyclone separation part (13), wherein said bottom outlet (17) of the hydrocyclone (5a; 5b; 5c; 5d, 5d?; 5e) is provided in a position such that when the bioreactor systeType: ApplicationFiled: March 25, 2020Publication date: April 28, 2022Inventors: Andreas Castan, Ricardo A. Medronho, Leda Castilho, Iona Walter Bettinardi
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Publication number: 20220034748Abstract: The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.Type: ApplicationFiled: September 9, 2019Publication date: February 3, 2022Inventors: Sameera Simha Hindupur, Manoj Kumar Ramakrishna, Andreas Castan, Swapnil Puranik, Klaus Gebauer
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Patent number: 11136598Abstract: The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GO genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.Type: GrantFiled: February 28, 2018Date of Patent: October 5, 2021Assignee: Cytiva Sweden ABInventors: Daniel Leif Ivansson, Andreas Castan
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Patent number: 11052165Abstract: The invention discloses a method for virus clearance of a cell culture medium, comprising the steps of: i) providing a bulk medium portion, comprising amino acids and glucose, and a first additive portion, comprising vitamins in aqueous solution; ii) subjecting the bulk medium portion to a high temperature short time treatment (HTST); iii) passing the first additive portion through a virus retentive filter or an ultrafilter; and iv) after steps ii) and iii), mixing the bulk medium portion with the first additive portion to obtain a cell culture medium.Type: GrantFiled: September 28, 2017Date of Patent: July 6, 2021Assignee: Global Life Sciences Solutions USA LLCInventors: Andreas Castan, Colin R. Tuohey
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Patent number: 10760041Abstract: The invention discloses a flexible bag for cell cultivation, comprising a cultivation compartment and at least one dialysis compartment, wherein the dialysis compartment(s) is/are delimited from the cultivation compartment by one or more dialysis membranes and is/are fluidically connected to a first and a second port in the bag.Type: GrantFiled: August 27, 2014Date of Patent: September 1, 2020Assignee: GE Healthcare Bio-Sciences ABInventors: Karl Liderfelt, Eric Faldt, Andreas Castan
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Patent number: 10731118Abstract: Provided is a bioreactor assembly that includes a plurality of cultivation bags at least one flexible temperature control bag having a thermostating fluid, and a tray adapted to rock back and forth around at least one axis. The flexible temperature control bag is mounted on the tray and the cultivation bags are located on the flexible temperature control bag.Type: GrantFiled: November 29, 2016Date of Patent: August 4, 2020Assignee: GE Healthcare Bio-Sciences Corp.Inventors: Ralph Stankowski, Asa Lundstrom, Andreas Castan, Michael Miller, Yvette Klingberg
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Publication number: 20200202051Abstract: The present invention relates to a method for predicting outcome of a process used for manufacturing a sample in a bioreactor, the process belonging to a category. The method comprises selecting (51) a process model based on the category; accessing (53) historic data related to past process runs for manufacturing the sample; accessing (53) current data obtained (54) from a current process run of the process. The obtained current data, which is based on the selected process model, comprises: process strategy data, bioreactor instrument data, data from online sensors and/or data from offline sensors. The method further comprises predicting (62) an outcome of at least one selected parameter of the current process run for manufacturing the sample based on the accessed historic data and current data. The present invention also relates to a method for modelling a process and a control system (10) for controlling a process.Type: ApplicationFiled: June 18, 2018Publication date: June 25, 2020Inventors: Krishna Kumar Swaminathan, Sridhar Dasaratha, Sittal George, Vinay Bhaskar Jammu, Alok Singh Chauhan, Nagaraju Konduru, Neelima Boddapati, Ankita Bhatia, Mithilesh Mohanty, Harsha Aeron, Andreas Castan
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Publication number: 20200087605Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.Type: ApplicationFiled: September 27, 2019Publication date: March 19, 2020Applicant: GE Healthcare Bio-Sciences ABInventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
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Publication number: 20200002728Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.Type: ApplicationFiled: February 23, 2018Publication date: January 2, 2020Inventors: Daniel Ivansson, Andreas Castan
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Patent number: 10450540Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.Type: GrantFiled: April 27, 2015Date of Patent: October 22, 2019Assignee: GE Healthcare Bio-Sciences ABInventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
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Publication number: 20180251790Abstract: The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GOI genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.Type: ApplicationFiled: February 28, 2018Publication date: September 6, 2018Inventors: Daniel Leif Ivansson, Andreas Castan