Abstract: Disclosed herein are spore compositions and methods of producing such compositions. Additionally disclosed herein are plant protection products benefiting from such spore compositions and methods of using such compositions for the benefit of plants, for the reduction of pathogen emissions to nearby areas and for the benefit of animals or humans. Further disclosed herein are methods of efficient fermentation.

Abstract: A method for determining optimized basis functions for describing trajectories. The method includes receiving reference data which describes possible trajectories; preprocessing the reference data, wherein the reference data is aggregated in a matrix Y; carrying out a singular value decomposition Y=USVT, wherein the matrices U and V each comprise singular vectors and S is a diagonal singular value matrix with singular values ?i; identifying at least one of the singular values ?i as a dominant singular value ?d,i and at least one other of the singular values ?i as a non-dominant singular value ?nd,i; and determining a matrix Ud which comprises dominant singular vectors assigned to the dominant singular values ?d,i, wherein the optimized basis functions are described by the dominant singular vectors.

Abstract: The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.

Abstract: The presently claimed invention is directed to a method for the preparation of an aqueous solution comprising at least one beta-glucan comprising at least the steps of a) fermentation of at least one fungal strain in an a fermentation broth, b) addition of at least one acid to the fermentation broth to adjust the pH to a value in the range of ?2.0 to ?4.0 and c) filtration of the fermentation broth to obtain an aqueous solution comprising the at least one beta-glucan and at least one beta-glucan that is obtained by this method.

Abstract: The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.

Abstract: The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.

Abstract: The presently claimed invention is directed to a method for the preparation of an aqueous solution comprising at least one beta-glucan comprising at least the steps of a) fermentation of at least one fungal strain in an a fermentation broth, b) addition of at least one acid to the fermentation broth to adjust the pH to a value in the range of ?2.0 to ?4.0 and c) filtration of the fermentation broth to obtain an aqueous solution comprising the at least one beta-glucan and at least one beta-glucan that is obtained by this method.

Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-?-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity or the use of such a polypeptide for producing ?-glucans. Furthermore, the present invention relates to methods for producing ?-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity into a microorganism being able to synthesize ?-glucans.

Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-?-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity or the use of such a polypeptide for producing ?-glucans. Furthermore, the present invention relates to methods for producing ?-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity into a microorganism being able to synthesize ?-glucans.

Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-?-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity or the use of such a polypeptide for producing ?-glucans. Furthermore, the present invention relates to methods for producing ?-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity into a microorganism being able to synthesize ?-glucans.

Abstract: The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.

Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-?-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity or the use of such a polypeptide for producing ?-glucans. Furthermore, the present invention relates to methods for producing ?-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity into a microorganism being able to synthesize ?-glucans.

Abstract: Described is the use of synthetic adhesives in the manufacture of corrugated fiberboard at relatively low temperatures and high lineal speeds. The corrugated fiberboard includes a corrugated sheet of paper and a flat linerboard and the corrugation of the corrugated sheet of paper is produced at paper temperatures below 95° C. and at a lineal speed above 150 m/min. The production of the corrugation of a corrugated sheet of paper is immediately followed by a continuous operation in which a preferably unheated corrugated board adhesive is applied and the corrugated sheet of paper is adhered to at least one first linerboard. The corrugated board adhesive used is an aqueous adhesive dispersion based on at least one synthetic, dispersed polymer having preferably more than 40% by weight solids content, selected from acrylate copolymers, copolymers of vinylaromatics and conjugated aliphatic dienes and vinyl acetate-alkylene copolymers, wherein the glass transition temperatures of the polymers are above 20° C.

Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.

Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.

Abstract: Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-?-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity or the use of such a polypeptide for producing ?-glucans. Furthermore, the present invention relates to methods for producing ?-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-?-D-glucan synthase-activity into a microorganism being able to synthesize ?-glucans.

Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a ?metF organism or a ?metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A ?metF ?metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

Abstract: The present invention relates to nulceotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof being modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.

Abstract: The present invention relates to a method of producing methionine in Coryneform bacteria in which enzymes of the pentose phosphate pathway are over-expressed. The present invention also relates to Coryneform bacteria for producing methionine in which at least two enzymes of the pentose phosphate pathway are over-expressed.