Abstract: Disclosed are various embodiments for growing, culturing, monitoring, and analyzing embryoid bodies, fused embryoid bodies, spheroids, organoids, or other multi-cellular bodies in a microwell structure formed in one or more wells of an assay and culturing microplate. Hydrogel deposited into a well of the microplate and supported by a support ledge and the bottom surface of the well is molded into a microwell structure using a mold insert tool. In some examples, channels can be formed in the bottom of the microwell structure to allow for an exchange of fluid between a primary well section and a secondary well section of the well. The bottom surface of the assay and culturing microplate is optically transparent and gas-permeable.

Abstract: The present disclosure provides a system, including methods and apparatus, for culturing, monitoring, and/or analyzing organoids. In an exemplary method of organoid culture, the method may comprise disposing a scaffold in a receptacle having an open side. A sealing member may be bonded to the open side of the receptacle to create a chamber. An organoid may be formed in the chamber using the scaffold. Fluid and/or at least one substance may be introduced into the chamber from an overlying reservoir for contact with the organoid.

Abstract: Systems, methods, and devices for culturing a multicellular structure, such as an organoid. An exemplary system comprises a vessel, an electric/magnetic module, and a control circuit. The vessel may include a culture chamber to contain a multicellular structure. The electric/magnetic module may be configured to be located in the vessel, at a position in or adjacent the culture chamber. The control circuit may be configured to wirelessly power and/or operate the electric/magnetic module.

Abstract: Methods, systems, and apparatus for cultivating cells in media-exchanging wells. In an exemplary method of cell cultivation, a device (50) may be selected that includes a row of wells (52), and a first reservoir (56) and a second reservoir (58) located at opposite ends of the row of wells (52). Each well (52) may have a lower portion and an upper portion. The lower portion of each well (52) of at least two of the wells (52) may contain a cell culture in contact with a liquid. Liquid may be transferred between the first reservoir (56) and the second reservoir (58) at least partly along a flow path (62) defined by the device and extending through the upper portion of each well (52) of the row of wells (52), such that molecules of the media are exchanged between or among the at least two wells (52).

Abstract: Disclosed are various embodiments for growing, culturing, monitoring, and analyzing embryoid bodies, fused embryoid bodies, spheroids, organoids, or other multi-cellular bodies using a system of microplates. Different types of microplates are designed to be used during the various stages of growing and culturing of cells to form embryoid bodies, fused embryoid bodies, spheroids, organoids, or other multi-cellular bodies. The different microplates are designed to mate with one another to allow for the transfer of cells from wells in one plate to wells in the other plate. An assay plate includes an array of perfusable units that include a supply well that is in fluid communication with a culture well to allow for an exchange of fluid.

Abstract: The present disclosure provides a system, including methods and apparatus, for culturing, monitoring, and/or analyzing organoids. In an exemplary method of organoid culture, the method may comprise disposing a scaffold in a receptacle having an open side. A sealing member may be bonded to the open side of the receptacle to create a chamber. An organoid may be formed in the chamber using the scaffold. Fluid and/or at least one substance may be introduced into the chamber from an overlying reservoir for contact with the organoid.

Abstract: Incubation system and method for automated cell culture and/or testing. An exemplary incubation system may comprise a housing forming a chamber. A rack may define storage positions to support an array of sample holders inside the chamber. A detection robot may be configured to capture one or more images of cells contained by one or more wells of each sample holder while the sample holder remains at one of the storage positions of the rack. A fluid handling station may be configured to add fluid to, and/or remove fluid from, one or more wells of each of the sample holders inside the housing. At least one plate robot may be configured to move sample holders between the rack and the fluid handling station. A computer may control operation of the detection robot, the fluid handling station, and the at least one plate robot.

Abstract: A light-field microscope includes a light-sheet focusing device configured for outputting illumination light as a light sheet, and a microlens array between an objective lens and a light detector. A sample is irradiated by the light sheet along a direction non-parallel to the sample plane. Light-field data may be acquired from the sample without needing to scan through the thickness of the sample. The microscope implements light-field acquisition in conjunction with selective plane illumination.

Abstract: A light-field microscope includes a light-sheet focusing device configured for outputting illumination light as a light sheet, and a microlens array between an objective lens and a light detector. A sample is irradiated by the light sheet along a direction non-parallel to the sample plane. Light-field data may be acquired from the sample without needing to scan through the thickness of the sample. The microscope implements light-field acquisition in conjunction with selective plane illumination.

Abstract: An arrangement for building a compact Fourier transform interferometer for optical radiation according to the Michelson principle or a principle derived therefrom. According to the invention, this arrangement is characterized in that the optical modulation of the radiation in at least one of the interferometer arms is produced by the translational displacement of micromechanical translation mirrors that are optically accessible on one side or on both sides, with an effective oscillation amplitude of at least 50 ?m and a usable mirror surface area of at least 1 mm2. In doing so, the micromechanical mirror components according to the invention assume the function of known movable mirrors so that by using an almost massless microcomponent in comparison to conventional systems, a much smaller size and a mirror modulation frequency that is several orders of magnitude higher can be achieved. The arrangement according to the invention is used especially for optical spectroscopy.

Abstract: An arrangement for building a compact Fourier transform interferometer for optical radiation according to the Michelson principle or a principle derived therefrom. According to the invention, this arrangement is characterized in that the optical modulation of the radiation in at least one of the interferometer arms is produced by the translational displacement of micromechanical translation mirrors that are optically accessible on one side or on both sides, with an effective oscillation amplitude of at least 50 ?m and a usable mirror surface area of at least 1 mm2. In doing so, the micromechanical mirror components according to the invention assume the function of known movable mirrors so that by using an almost massless microcomponent in comparison to conventional systems, a much smaller size and a mirror modulation frequency that is several orders of magnitude higher can be achieved. The arrangement according to the invention is used especially for optical spectroscopy.