Abstract: The present invention relates to a combination of antibodies comprising (a) an anti-HPV-16 E7 antibody obtainable by (i) eliciting an in vivo humoral response against HPV-16 E7 protein or a fragment thereof in a goat; and (ii) affinity-purifying antibodies as obtained in the eliciting-step (i) and (b) an anti-HPV-18 E7 antibody. Additionally, in another aspect the present invention relates to methods for producing said combination of antibodies. Furthermore, the invention provides for the use of the combination of antibodies or for the use of an anti-HPV-16 E7 antibody obtainable as mentioned above for the preparation of a diagnostic composition for the (immuno-) histological detection of high risk HPV in a biological sample. Additionally, the invention relates to diagnostic compositions comprising said combination of antibodies or said anti-HPV-16 E7 antibody obtainable as mentioned above as well as to methods for producing said diagnostic compositions.

Abstract: The present invention relates to an anti-HPV-16 E7 antibody obtainable by (a) eliciting an in vivo humoral response against highly purified HPV-16 E7 protein or a fragment thereof in a non-human vertebrate; and (b) affinity-purifying antibodies as obtained in the eliciting-step (a). Furthermore, the invention provides for the use of the anti-HPV-1 E7 antibody/antibodies for the preparation of a diagnostic composition of the (immuno-)histological detection of expressed HPV-16 E7 in a biological sample. Additionally, the invention relates to specific diagnostic compositions as well as to methods producing the same. The invention also provides for kits comprising (an) anti-HPV-16 E7 antibody(ies) of the invention or a diagnostic composition and discloses in vitro methods for the detection of a sexually transmittable disease or cancer, in particular cervical cancer, breast cancer, prostate cancer, anogenital cancer/neoplasia, penile cancer or head and neck cancer by using the described antibodies.