Patents by Inventor Andrei Alexeev

Andrei Alexeev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10557166
    Abstract: The present invention provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: February 11, 2020
    Assignee: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Patent number: 10494630
    Abstract: Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5? end of the long strand has a phosphoric acid modification, and the 3? end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3? end thereof can be in a complementary pairing with the 5? end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: December 3, 2019
    Assignee: MGI TECH CO., LTD.
    Inventors: Yuan Jiang, Chunyu Geng, Xia Zhao, Shujin Fu, Lingyu He, Yaqiao Li, Xiaoshan Su, Fanzi Wu, Wenwei Zhang, Hui Jiang, Andrei Alexeev, Radoje Drmanac
  • Patent number: 10479991
    Abstract: A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: November 19, 2019
    Assignee: MGI TECH CO., LTD
    Inventors: Yuan Jiang, Qiaoling Li, Andrei Alexeev, Evan Hurowitz, Xia Zhao, Tong Wang, Chao Dong, Dong Li, Radoje Drmanac, Wenwei Zhang, Hui Jiang
  • Publication number: 20190002969
    Abstract: The present invention provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Application
    Filed: March 26, 2018
    Publication date: January 3, 2019
    Applicant: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Publication number: 20190002970
    Abstract: This disclosure provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Application
    Filed: May 30, 2018
    Publication date: January 3, 2019
    Applicant: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Publication number: 20180355421
    Abstract: The present invention provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Application
    Filed: March 17, 2014
    Publication date: December 13, 2018
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Patent number: 10023910
    Abstract: This disclosure provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: July 17, 2018
    Assignee: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Publication number: 20180080092
    Abstract: Disclosed are a one-stop treatment method for breaking a nucleic acid by means of a transposase, and a reagent. The method of the present invention comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, the transposase-embedded complex comprising a transposase and a first adaptor comprising a transposase identification sequence; adding a first reagent to conduct treatment, so as to break an absorption effect of the transposase to a target sequence of the nucleic acid; adding a second reagent to conduct treatment, so as to weaken the influence of the first reagent on a follow-up enzyme-catalyzed reaction; and conducting a PCR reaction by using a product generated after the second reagent treatment as a template component, so as to obtain a PCR product of a broken nucleic acid segment whose two ends are connected to adaptors.
    Type: Application
    Filed: October 14, 2014
    Publication date: March 22, 2018
    Applicant: BGI SHENZHEN CO., LIMITED
    Inventors: Chunyu GENG, Rongrong GUO, Ruoying CHEN, Yingxin ZHANG, Andrei ALEXEEV, Hui JIANG, Wenwei ZHANG
  • Publication number: 20180044668
    Abstract: The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are also provided.
    Type: Application
    Filed: October 13, 2015
    Publication date: February 15, 2018
    Applicant: BGI SHENZHEN CO., LIMITED
    Inventors: Yuan JIANG, Radoje DRMANAC, Evan HUROWITZ, Andrei ALEXEEV, Xia ZHAO, Jie RUAN
  • Publication number: 20170355981
    Abstract: A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library.
    Type: Application
    Filed: November 26, 2014
    Publication date: December 14, 2017
    Applicants: BGI SHENZHEN, BGISHENZHEN CO., LIMITED
    Inventors: Yuan JIANG, Qiaoling LI, Andrei ALEXEEV, Evan HUROWITZ, Xia ZHAO, Tong WANG, Chao DONG, Dong LI, Radoje DRMANAC, Wenwei ZHANG, Hui JIANG
  • Publication number: 20170349893
    Abstract: A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library.
    Type: Application
    Filed: November 26, 2014
    Publication date: December 7, 2017
    Applicants: BGI SHENZHEN, BGI SHENZHENN CO., LIMITED
    Inventors: Yuan JIANG, Xia ZHAO, Andrei ALEXEEV, Radoje DRMANAC, Wenwei ZHANG, Hui JIANG
  • Publication number: 20170292153
    Abstract: Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent.
    Type: Application
    Filed: October 14, 2014
    Publication date: October 12, 2017
    Applicant: BGI SHENZHEN CO., LIMITED
    Inventors: Chunyu GENG, Ruoying CHEN, Rongrong GUO, Andrei ALEXEEV, Yingxin ZHANG, Hui JIANG, Wenwei ZHANG
  • Publication number: 20170233728
    Abstract: Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5? end of the long strand has a phosphoric acid modification, and the 3? end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3? end thereof can be in a complementary pairing with the 5? end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
    Type: Application
    Filed: October 14, 2014
    Publication date: August 17, 2017
    Inventors: Yuan JIANG, Chunyu GENG, Xia ZHAO, Shujin FU, Lingyu HE, Yaqiao LI, Xiaoshan SU, Fanzi WU, Wenwei ZHANG, Hui JIANG, Andrei ALEXEEV, Radoje DRMANAC
  • Publication number: 20170120213
    Abstract: The present invention is directed to methods and compositions for long fragment read sequencing. The present invention encompasses methods and compositions for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data.
    Type: Application
    Filed: October 10, 2016
    Publication date: May 4, 2017
    Applicant: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev, Peter Hong
  • Publication number: 20170022554
    Abstract: This disclosure provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Application
    Filed: April 22, 2016
    Publication date: January 26, 2017
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Patent number: 9499863
    Abstract: The present invention is directed to methods and compositions for long fragment read sequencing. The present invention encompasses methods and compositions for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data.
    Type: Grant
    Filed: September 16, 2013
    Date of Patent: November 22, 2016
    Assignee: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev, Peter Hong
  • Patent number: 9328382
    Abstract: This disclosure provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: May 3, 2016
    Assignee: COMPLETE GENOMICS, INC.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Publication number: 20160046985
    Abstract: The present invention provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 18, 2016
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev
  • Publication number: 20150155441
    Abstract: LED (“light emitting diode”) package (1) comprising a substrate (2) with a top side (9) and a bottom side (10), and at least one LED die (4), the substrate (2) having circuitry (3) arranged on its bottom side (10), the at least one LED die (4) comprising a bottom surface (6) exhibiting at least two separated contact areas (7, 8) for electrical connection. In order to realise an LED package (1) with mechanically robust electrical connections that can be simultaneously produced, according to the present invention, it is provided that the at least one LED die (4) is at least partially arranged in the substrate (2), and that at least one of the at least two contact areas (7, 8) is electrically connected to the circuitry (3) by a contact electrode (11) consisting of a film of conductive material (22).
    Type: Application
    Filed: June 15, 2012
    Publication date: June 4, 2015
    Inventors: Andrei Alexeev, Sergey Popper
  • Publication number: 20140323316
    Abstract: This disclosure provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example.
    Type: Application
    Filed: March 11, 2014
    Publication date: October 30, 2014
    Applicant: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Brock A. Peters, Andrei Alexeev