Patents by Inventor Andrei Darievich Mirzabekov
Andrei Darievich Mirzabekov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7846656Abstract: The invention refers to the field of molecular biology and bio-organic chemistry and deals with a composition for immobilization in polymer carriers of oligonucleotides, proteins, nucleic acids or any other molecules, which structure comprises active groups, including amino- or sulfhydryl groups, as well as a method for immobilization of various molecules including oligonucleotides, proteins, nucleic acids or any other molecules which structure comprises active groups, including amino- or sulfhydryl groups, consisting of a porous polymer being obtained on the basis of the composition claimed under conditions of addition or substitution reaction (radical, nucleophilic, or electrophilic nature, etc.) during synthesis of polymer by photo- and chemical initiated polymerization.Type: GrantFiled: October 16, 2001Date of Patent: December 7, 2010Assignee: Institut Molekulyarnoi Biologii IM.V.A. Engelgardta Rossiiskoi Akademii NaukInventors: Andrei Darievich Mirzabekov, Alla Jurievna Rubina, Sergei Vasilievich Pankov
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Publication number: 20100261163Abstract: The present invention relates to molecular biology, microbiology, and medicine and provides the method for detection of Mycobacterium tuberculosis complex with simultaneous evaluation of sensitivity of the strains to rifampicin and isoniazid in clinical sample on differentiating biochip. The method is based on two-stage multiplex PCR to obtain fluorescent DNA fragments followed by hybridization of these fragments on microarray containing the set of specific discriminating oligonucleotides. The determination of the resistance of Mycobacterium tuberculosis to rifampicin and isoniazid is carried out by evaluation of point nucleotide substitutions in DNA of microorganism. The present invention allows conduct analysis directly in clinical sample, to evaluate a number of mutations simultaneously, to decrease the cost price of analysis, and to reduce the time of its conducting. The present invention also relates to set of primers, biochip, and set of oligonucleotide probes used in realization of the method.Type: ApplicationFiled: December 26, 2006Publication date: October 14, 2010Inventors: Alexandr Sergeevich Zasedatelev, Alexander Yurievich Sobolev, Dmitry Alexandrovich Gryadunov, Sergei Anatolievich Lapa, Vladimir Mikhailovich Mikhailovich, Andrei Darievich Mirzabekov, Natalia Vladimirovna Mirzabekova
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Publication number: 20040241713Abstract: The invention relates to molecular biology and bioorrganic chemistry and concerns compositions for immobilising modified oligonucleotides, proteins, nucleic acids and any other molecules carrying unsaturated groups in a hydrogel for producing microchips by means of a photo-induced copolymerisation. Said invention also relates to the production of microchips and a polymerase chain reaction (PCR) on a chip which are used in molecular biology for sequencing and mapping DNA, detecting mutations and for an entire range of medical applications.Type: ApplicationFiled: January 23, 2004Publication date: December 2, 2004Applicant: INSTITUT MOLEKULYARNOI BIOLOGII IM. V.A. ENGELGARDTA ROSSIISKOI AKADEMII NAUKInventors: Andrei Darievich Mirzabekov, Alla Jurievna Rubina, Sergei Vasilievich Pankov, Alexandr Nikolaevich Perov, Valentina Vladimirovna Chupeeva
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Publication number: 20040053298Abstract: The invention refers to the field of molecular biology and bio-organic chemistry and deals with a composition for immobilization in polymer carriers of oligonucleotides, proteins, nucleic acids or any other molecules, which structure comprises active groups, including amino- or sulfhydryl groups, as well as a method for immobilization of various molecules including oligonucleotides, proteins, nucleic acids or any other molecules which structure comprises active groups, including amino- or sulfhydryl groups, consisting of a porous polymer being obtained on the basis of the composition claimed under conditions of addition or substitution reaction (radical, nucleophilic, or electrophilic nature, etc.) during synthesis of polymer by photo- and chemical initiated polymerization.Type: ApplicationFiled: October 28, 2003Publication date: March 18, 2004Inventors: Andrei Darievich Mirzabekov, Alla Jurievna Rubina, Sergei Vasilievich Pankov
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Patent number: 6465174Abstract: A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to polymerize into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.Type: GrantFiled: July 31, 1998Date of Patent: October 15, 2002Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Gennadiy Moiseyevich Yershov, Dmitry Yuryevich Guschin, Margaret Anne Gemmell, Valentine V. Shick, Dmitri Y. Proudnikov, Edward N. Timofeev
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Patent number: 6440671Abstract: A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength.Type: GrantFiled: February 7, 2000Date of Patent: August 27, 2002Assignee: The University of ChicagoInventors: Andrei Darievich Mirzabekov, Eugene Vladislavovich Kirillov, Sergei Valeryevich Parinov, Victor Evgenievich Barski, Svetlana Alekseevna Dubiley
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Patent number: 6143499Abstract: A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers.Type: GrantFiled: June 19, 1998Date of Patent: November 7, 2000Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Yuri Petrovich Lysov, Svetlana A. Dubley
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Patent number: 6090549Abstract: A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength.Type: GrantFiled: May 13, 1997Date of Patent: July 18, 2000Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Eugene Vladislavovich Kirillov, Sergei Valeryevich Parinov, Victor Evgenievich Barski, Svetlana Alekseevna Dubiley
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Patent number: 5981734Abstract: A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.Type: GrantFiled: July 17, 1997Date of Patent: November 9, 1999Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Dimitri Y. Proudnikov, Edward N. Timofeev, Svetlana V. Kochetkova, Vladimir L. Florentiev, Valentine V. Shick
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Patent number: 5962329Abstract: A method and a device for dispensing microdoses of aqueous solutions are provided, whereby the substance is transferred by the free surface end of a rodlike transferring element; the temperature of the transferring element is maintained at essentially the dew point of the ambient air during the transfer. The device may comprise a plate-like base to which are affixed a plurality of rods; the unfixed butt ends of the rods are coplanar. The device further comprises a means for maintaining the temperature of the unfixed butt ends of the rods essentially equal to the dew point of the ambient air during transfer of the aqueous substance.Type: GrantFiled: April 16, 1998Date of Patent: October 5, 1999Assignee: University of ChicagoInventors: Gennady Moiseevich Ershov, Eugenii Vladislavovich Kirillov, Andrei Darievich Mirzabekov
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Patent number: 5908745Abstract: A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength.Type: GrantFiled: January 16, 1996Date of Patent: June 1, 1999Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Gennadiy Moseyevich Yershov, Eugene Vladislavovich Kirillov, Sergei Valeryevich Parinov, Victor Evgenievich Barski, Yuri Petrovich Lysov
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Patent number: 5905024Abstract: A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers.Type: GrantFiled: December 17, 1996Date of Patent: May 18, 1999Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Yuri Petrovich Lysov, Svetlana A. Dubley
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Patent number: 5861247Abstract: A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.Type: GrantFiled: January 26, 1996Date of Patent: January 19, 1999Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Edward Nikolaevich Timofeev, Vladimer Leonidovich Florentiev, Eugene Vladislavovich Kirillov
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Patent number: 5851772Abstract: A method for enriching specific genetic material sequences is provided, whereby oligonucleotide molecules complementary to the desired genetic material is first used to isolate the genetic material from a first source of genomic material. Then the genetic material is used as a label to isolate similar genetic sequences from other sources.Type: GrantFiled: January 29, 1996Date of Patent: December 22, 1998Assignee: University of ChicagoInventors: Andrei Darievich Mirzabekov, Yuri Petrovich Lysov, Valentine Vladimirovich Shick, Svetlana Alekseevna Dubiley
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Patent number: 5770721Abstract: This method for preparing micromatrices consists in applying a specially-patterned intermediate layer of laser-absorbing substance on a solid support. The configuration of the sublayer fully corresponds to the topology of the manufactured matrix. The intermediate layer is further covered by a continuous layer of gel , the gel and the material of the support being transparent towards laser radiation. The gel layer is irradiated by a laser beam for a time needed to evaporate simultaneously the gel in the places immediately above the laser-absorbing sublayer and the sublayer itself. Oligonucleotides from a chosen set are then attached to the formed gel `cells`, one oligonucleotide to each cell.This method is intended for use in biotechnology, specifically for deciphering the nucleotide sequence of DNA.Type: GrantFiled: May 19, 1995Date of Patent: June 23, 1998Assignee: University of ChicagoInventors: Gennady Moiseevich Ershov, Andrei Darievich Mirzabekov
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Patent number: 5756050Abstract: A device for dispensing microdoses of aqueous solutions are provided, whereby the substance is transferred by the free surface end of a rodlike transferring element; the temperature of the transferring element is maintained at essentially the dew point of the ambient air during the transfer. The device may comprise a plate-like base to which are affixed a plurality of rods; the unfixed butt ends of the rods are coplanar. The device further comprises a means for maintaining the temperature of the unfixed butt ends of the rods essentially equal to the dew point of the ambient air during transfer of the aqueous substance.Type: GrantFiled: May 26, 1995Date of Patent: May 26, 1998Assignee: University of ChicagoInventors: Gennady Moiseevich Ershow, Evgenii Vladislavovich Kirillov, Andrei Darievich Mirzabekov
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Patent number: 5741700Abstract: The method for immobilizing water-soluble bioorganic compounds to capillary-porous carrier comprises application of solutions of water-soluble bioorganic compounds onto a capillary-porous carrier, setting the carrier temperature equal to or below the dew point of the ambient air, keeping the carrier till appearance of water condensate and complete swelling of the carrier, whereupon the carrier surface is coated with a layer of water-immiscible nonluminescent inert oil and is allowed to stand till completion of the chemical reaction of bonding the bioorganic compounds with the carrier.Type: GrantFiled: June 2, 1995Date of Patent: April 21, 1998Assignee: University of ChicagoInventors: Gennady Moiseevich Ershov, Eduard Nikolaevich Timofeev, Igor Borisovich Ivanov, Vladimir Leonidovich Florentiev, Andrei Darievich Mirzabekov