Abstract: The present disclosure is related to the fields of biology, molecular biology, genetics, microbial fermentation, alcohol production and the like. The present compositions and methods relate to yeast strains comprising genetic modifications that results in modified yeast strains thereof comprising enhanced stress tolerance. Certain embodiments of the disclosure are therefore related to compositions and methods for increasing the efficiency of alcohol production using such modified yeast strains in fermentation reactions/processes.

Abstract: The instant disclosure is generally related to microbial fermentation, grain processing and ethanol production. Certain embodiments of the disclosure are therefore related to starch-containing materials and the microbial fermentation of such starch-containing materials for the production ethanol. More particularly, certain embodiments are related to novel protease mixtures for use in processing such starch-containing materials in starch to ethanol fermentation processes described herein.

Abstract: A method for the production of a hydrolysate comprising: (a) admixing at least one protein or a portion thereof with: (A) at least one endoprotease; and (B) (a?) at least one proline tolerant tripeptidyl peptidase or fermentate comprising a proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at P1; and synthetic amino acids at P1; or (b?) at least one proline tolerant tripeptidyl peptidase having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at PV; and synthetic amino acids at PV; and (b) recovering the hydrolysate.

Abstract: The present disclosure is related to the fields of biology, molecular biology, genetics, microbial fermentation, alcohol production and the like. The present compositions and methods relate to yeast strains comprising genetic modifications that results in modified yeast strains thereof comprising enhanced stress tolerance. Certain embodiments of the disclosure are therefore related to compositions and methods for increasing the efficiency of alcohol production using such modified yeast strains in fermentation reactions/processes.

Abstract: Described is yeast having reduced acetate production as a consequence of expressing selected heterologous acetyl-Co synthase (ACS) enzymes. Such yeast may further be modified to reduce glycerol and/or increased ethanol production. The yeast is useful for producing ethanol from carbohydrate-containing substrates.

Abstract: Described are compositions and methods relating to modified yeast that over-express protein phosphatases associated with the HOG pathway. The yeast produces an increased amount of ethanol and deceased amount of glycerol compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates.

Abstract: A method of preparing a feed additive composition comprising: (a) admixing at least one proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having (i) (A) Proline at P1; and (B) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids at P1; or (ii) (a?) Proline at PV; and (b?) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids or amines at PV; and one or more ingredients selected from the group consisting of a salt, polyol including sorbitol and glycerol, whe

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract: Described are composition and methods relate to reducing the redox imbalance in anaerobically growing yeast with attenuated glycerol production by re-engineering the pathway for Ac-CoA biosynthesis.

Abstract: Described are methods for increasing the expression of a transgene in eukaryotic cells by reducing RNA interference (RNAi), and variant cells produce by the method. The methods and variant cells are useful, for example, for the efficient production of therapeutic and industrial polypeptides in eukaryotic cells.

Abstract: A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.

Abstract: A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.

Abstract: A method of preparing a feed additive composition comprising: (a) admixing at least one proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having (i) (A) Proline at P1; and (B) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids at P1; or (ii) (a?) Proline at P1?; and (b?) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids or amines at P1?; and one or more ingredients selected from the group consisting of a salt, polyol including sorbitol and glycerol, w

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract: Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and Trichoderma host cells having a modification comprising or consisting essentially of disruption or deletion of nucleotide(s) for expression of this cellulose, whereby EGV expression is prevented.

Abstract: A method for the production of a hydrolysate comprising: (a) admixing at least one protein or a portion thereof with: (A) at least one endoprotease; and (B) (a?) at least one proline tolerant tripeptidyl peptidase or fermentate comprising a proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at P1; and synthetic amino acids at P1; or (b?) at least one proline tolerant tripeptidyl peptidase having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at PV; and synthetic amino acids at PV; and (b) recovering the hydrolysate.

Abstract: The present invention provides a method for producing an alcohol comprising: (a) admixing a tripeptidyl peptidase, predominantly having exopeptidase activity, with a feedstock or a fraction thereof before, during or after fermentation of said feedstock or a fraction thereof; and (b) recovering an alcohol. Also provided are uses of a tripeptidyl peptidase and by-products of alcohol production obtainable by the method of the invention.

Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Abstract: The present teachings provide large scale genetically engineered yeast in active dry form.