Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13. Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.

Abstract: A novel device and method for characterization of molecules is provides that improves characterization accuracy by utilizing larger numbers of reactive molecules that are smaller or shorter in chain length for the analysis procedure. Modification of markers such as nanotubes form nanotube assemblies that are easily detected using a number of surface analysis devices such as AFM and STM. The novel method shown using carbon nanotubes to mark a signature on reactive molecules permits a larger distribution and smaller molecule size of reactive molecules used in characterization of a sample molecule. The modification of the carbon nanotubes allows the characterization procedure to detect the nanotube markers more easily, thus decreasing characterization errors, and allowing faster characterization speeds.

Abstract: The present invention relates to a method for controlling non-linear coupled systems with a single output. More particularly, the present invention relates to control and coordination of force actuators that act on a single object without use of a central controller.

Abstract: Distributed market based analog control of a system of actuators and sensors. The system is comprised of one or more producing units having an output responsive to a market price and one or more input units having an input responsive to a market price. Market price information is communicated through an information channel that is encoded as measurable changes in non-electrical partitionable physical properties. Such partitionable physical properties include pressure, thermal, chemical or acoustic properties.

Abstract: A microsensor including a VCSEL for use in a MEMS. By coupling the top mirror of the VCSEL to the micromechanical structure, either directly or indirectly, the motion of the micromechanical structure in response to a physical phenomenon can directly modulate the wavelength of the light emitted from the VCSEL. Also, a method for sensing and transmitting information about the configuration or motion of a mechanical structure. The method includes coupling the top mirror of a VCSEL to the mechanical structure that directly encodes information about the motion into the frequency of light emitted by the VCSEL.

Abstract: The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.

Abstract: The disclosed methods, apparatus and compositions are of use for nucleic acid sequencing. More particularly, the methods and apparatus concern sequencing single molecules of single stranded DNA or RNA by exposing the molecule to exonuclease activity, removing free nucleotides one at a time from one end of the nucleic acid, and identifying the released nucleotides by Raman spectroscopy or FRET.

Abstract: An air jet game comprising an air jet conduiting member having a plurality of air jet outlets and a controller adapted to selectively control at least partially, the flow of air out of the air jet outlets in order to move an object located in an air flow path of the outlet in a desired direction.

Abstract: A method of marking is disclosed in which a propellant stream is passed through a channel and directed toward a substrate. Liquid marking material, such as ink, is controllably introduced into the propellant stream and imparted with sufficient kinetic energy thereby to be made incident upon a substrate. A multiplicity of channels for directing the propellant and marking material allow for high throughput, high resolution marking. Multiple marking materials may be introduced into the channel and mixed therein prior to being made incident on the substrate, or mixed or superimposed on the substrate without registration. One example is a single-pass, full-color printer.

Abstract: A MEMS-based system that enables low cost, low power, single chip realization of real-time signal detection and fault diagnosis. The system is suitable for analyzing the time-varying properties of a signal that are important for condition-based monitoring of electro-mechanical machines or structures. The system includes mechanical sensors that sense input signals such as vibration, signal templates of fault conditions, logic units that detect, store, and compare signal features to provide a diagnostic state, and an output readout mechanism to couple the diagnostic state to readout device that provides an external signal.

Abstract: An air jet game comprising an air jet conduiting member having a plurality of air jet outlets and a controller adapted to selectively control at least partially, the flow of air out of the air jet outlets in order to move an object located in an air flow path of the outlet in a desired direction.

Abstract: A micromechanical resonator including a motion arresting mechanism to rapidly damp the vibration of a resonator beginning at any given moment in time to remove vibration caused by previous events. An electrostatic clamp uses a bias voltage between an electrode and the resonator to damp the resonator and return it to its equilibrium position. A mechanical clamp includes an actuator that forces the mechanical clamp to contact the resonator. These micromechanical resonators facilitate condition based monitoring of complex electromechanical machines and components by allowing signature analysis in multiple temporal and frequency domains.

Abstract: A marking apparatus is disclosed in which a propellant stream is passed through a channel and directed toward a substrate. A liquid marking material, such as ink, is controllably introduced into the propellant stream and imparted with sufficient kinetic energy thereby to be made incident upon a substrate. A multiplicity of channels for directing the propellant and marking material allow for high throughput, high resolution marking. Multiple marking materials may be introduced into the channel and mixed therein prior to being made incident on the substrate, or mixed or superimposed on the substrate without registration. One example is a single-pass, full-color printer.

Abstract: A bistable sensor with a tunable threshold for use in microelectromechanical systems. The sensor uses electrostatic force to modify the threshold and to disable the sensor in a deflected position once a sustained extreme in vibration is detected. Potential applications include mechanical implementations of signature analysis to automatically eliminate large amplitude noise at a specific frequency, shock detection without requiring quiescent DC power consumption, and determination of the magnitude of a shock.

Abstract: Batch fabrication of thin film structures can be facilitated by sandwiching a thin film between a first and a second polymeric or elastomeric layers. The sandwiched layer can be machined to define a thin film structure, typically a micoroelectromechanical element. This element is separated from the sandwiching layers by adhesive attachment to a target substrate.

Abstract: Construction of fluid conduit systems in printed circuit boards or other dielectric laminate substrates is disclosed. The fluid conduits can be angled or curved to provide greater directional control of fluid flow. Conduits are created by lamination of a first laminate layer and a second laminate layer. The first laminate layer is composed of a dielectric base material impregnated with a resin, with a first aperture defined therethrough, while the second laminate layer has a second aperture. The second aperture is positioned with respect to the first aperture to only partially overlap, together defining an angled conduit. Fluid flow through the conduits can be controlled using microdevice valves.

Abstract: A market based controller controls the motion of an object on a transport assembly. The transport assembly is formed using sensors and actuators that are proximately coupled in physical space. To efficiently allocate a fixed amount of air pressure directed to the actuators, the market controller maps each market agent to points in space on the transport assembly, as well as, points in time relative to a system clock. Using information gathered from the sensor units, each market agent determines whether to bid on the fixed amount of air pressure each bid interval. Using the bids submitted by the market agents, a market equilibrium price and quantity are computed by a market auctioneer. The air pressure purchased by each market agent is then distributed to the actuators mapped thereto.

Abstract: A valve for redirecting fluid flow has a valve chamber supporting fluid flow, with the valve chamber having an inlet, a first outlet, and a second outlet. The valve chamber can be closed or partially open. The flap element is movable to alternatively block the first outlet and the second outlet. In or immediately adjacent to the valve chamber are opposing first and a second catch mechanisms for controllably latching the flap element to block respectively the first outlet and the second outlet. The first and second catch mechanisms have a disabled state and an activated state for holding and allowing release of the flap element. Once the first or second catch mechanism is disabled, the flap element is free to move to another position, provided it can overcome the mechanical fluid flow forces that tend to hold it in position.

Abstract: A system for detecting and damping vibrations in printer components, mechanical devices, buildings, or large structures has a plurality of light beam detectors for generating signals corresponding to a reference light beam position. The light beam detectors are partially transparent to allow passage of the light beam through the detector, permitting multiple detectors to use the same reference light beam. The detectors are attached to vibration susceptible structural elements, with detected movement of the light beam with respect to the partially transparent light beam detector corresponding to movement of the vibration susceptible structural element. Motion control units connected to the detectors can be used to control or damp detected vibrations in real-time.

Abstract: A valve array for supporting objects such as paper with controlled fluid flow is disclosed. Optional valve arrays can be used to controllably eject marking agents for marking or coating a paper or other suitable substrate. Each valve in the valve array has one or more flap elements positioned in a valve chamber. These flap elements are movable to alternatively block the fluid flow through valve chamber outlets with the aid of various catch mechanisms for controllably latching the flap elements. The catch mechanisms have a disabled state and an activated state for holding and allowing release of the flap elements. Once the catch mechanism are disabled, the flap elements are free to move to another position, provided they can overcome the mechanical fluid flow forces that tend to hold it in position. To counter and utilize such forces for moving the flap elements, an impulse mechanism kicks the flap element into the valve chamber, away from the catch mechanisms.