Patents by Inventor Andrew B. Feldman
Andrew B. Feldman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11952622Abstract: Methods for analyzing DNA-containing samples are provided. The methods can comprise isolating a single genomic equivalent of DNA from the DNA-containing sample to provide a single isolated DNA molecule. The single isolated DNA molecule can be subjected to amplification conditions in the presence of one or more sets of unique molecularly tagged primers to provide one or more amplicons. Any spurious allelic sequences generated during the amplification process are tagged with an identical molecular tag. The methods can also include a step of determining the sequence of the one or more amplicons, in which the majority sequence for each code is selected as the sequence of the single original encapsulated target. The DNA-containing sample can be a forensic sample (e.g., mixed contributor sample), a fetal genetic screening sample, or a biological cell.Type: GrantFiled: July 15, 2014Date of Patent: April 9, 2024Assignee: The Johns Hopkins UniversityInventors: Andrew B. Feldman, Jeffrey S. Lin, David Weitz, Assaf Rotem
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Patent number: 11660600Abstract: A microfluidic assembly may include a microfluidic chip operably coupled to a device source pressure port and a device relief pressure port, first and second input reservoirs, an output reservoir, and a reservoir interface. The microfluidic chip may include a microfluidic circuit configured to support a fluid flow that includes a gas flow and a liquid flow within the microfluidic circuit. The reservoir interface may be configured to operably couple the first and second input reservoirs to the microfluidic circuit. The device source pressure port may be configured to receive a source pressure to generate the fluid flow through the microfluidic circuit and cause a mixing of liquids to form an output liquid for delivery to the output reservoir via the fluid flow. The first liquid, the second liquid, and the output liquid need not contact the device source pressure port or the device relief pressure port during the mixing.Type: GrantFiled: July 28, 2021Date of Patent: May 30, 2023Assignee: The Johns Hopkins UniversityInventors: Jeffrey S. Lin, Andrew B. Feldman
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Publication number: 20230034468Abstract: A microfluidic assembly may include a microfluidic chip operably coupled to a device source pressure port and a device relief pressure port, first and second input reservoirs, an output reservoir, and a reservoir interface. The microfluidic chip may include a microfluidic circuit configured to support a fluid flow that includes a gas flow and a liquid flow within the microfluidic circuit. The reservoir interface may be configured to operably couple the first and second input reservoirs to the microfluidic circuit. The device source pressure port may be configured to receive a source pressure to generate the fluid flow through the microfluidic circuit and cause a mixing of liquids to form an output liquid for delivery to the output reservoir via the fluid flow. The first liquid, the second liquid, and the output liquid need not contact the device source pressure port or the device relief pressure port during the mixing.Type: ApplicationFiled: July 28, 2021Publication date: February 2, 2023Inventors: Jeffrey S. Lin, Andrew B. Feldman
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Patent number: 10524493Abstract: Food products and/or pharmaceutical preparations including (i) viral neutralizing antibodies or antibody fragments anchored to a probiotic microorganism and (ii) a carrier medium for delivering the viral neutralizing antibodies or antibody fragments anchored to probiotic microorganisms to the gut of a mammal. Also provided are methods of making food products and/or pharmaceutical preparations, which can be used to treat existing viral infections or prevent the spread or transmission of viral infection.Type: GrantFiled: September 16, 2014Date of Patent: January 7, 2020Assignee: The Johns Hopkins UniversityInventors: Andrew B. Feldman, Jeffrey S. Lin, Kellogg Schwab, Timothy Julian, Christiane Wobus, David Weitz, John Heyman
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Patent number: 9920128Abstract: A method for synthesizing an antiserum for rapid-turnaround therapies includes collecting antibody-secreting cells from a test subject, wherein the test subject has been exposed to a target biological agent and has produced an antibody response; selecting a subset of the antibody-secreting cells, the subset of the antibody-secreting cells producing antibodies that neutralize the target biological agent; generating variable-region-coding DNA sequences from the antibodies that neutralize the target biological agent; tagging amplicons of the variable-region-coding DNA sequences with unique nucleic acid identifiers to associate the variable-region-coding DNA sequences derived from individual ones of the subset of the antibody-secreting cells; analyzing antibody-type distribution in a natural immune response; synthesizing antibodies from the variable-region-coding DNA sequences to form synthetic antibodies; and mixing the synthetic antibodies in a proportion equal to the antibody-type distribution in the natural iType: GrantFiled: January 20, 2016Date of Patent: March 20, 2018Assignee: The Johns Hopkins UniversityInventors: Jeffrey S. Lin, Andrew B. Feldman, Jared D. Evans, Joshua T. Wolfe, David Weitz, John Heyman, Andrew S. Pekosz
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Patent number: 9696295Abstract: Certain embodiments of the present invention include a method for rapidly designing pharmaceutical preparations for preventing viral infection that have adapted to growth in various culturing systems but maintain antigenic similarity to the original virus. The method may include serial passaging a plurality of instances of a targeted virus strain from an infected first animal in respective droplets in second animal cells, in parallel synthesizing antibodies that neutralize the targeted virus strain in a third animal, and selecting the second animal cell-adapted viral lineages that are neutralized by the antibodies to identify second animal cell-adapted viral lineages having antigenic similarity to the targeted virus strain.Type: GrantFiled: January 18, 2017Date of Patent: July 4, 2017Assignee: The Johns Hopkins UniversityInventors: Jeffrey S. Lin, Andrew S. Pekosz, Andrew B. Feldman
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Publication number: 20170122933Abstract: Certain embodiments of the present invention include a method for rapidly designing pharmaceutical preparations for preventing viral infection that have adapted to growth in various culturing systems but maintain antigenic similarity to the original virus. The method may include serial passaging a plurality of instances of a targeted virus strain from an infected first animal in respective droplets in second animal cells, in parallel synthesizing antibodies that neutralize the targeted virus strain in a third animal, and selecting the second animal cell-adapted viral lineages that are neutralized by the antibodies to identify second animal cell-adapted viral lineages having antigenic similarity to the targeted virus strain.Type: ApplicationFiled: January 18, 2017Publication date: May 4, 2017Inventors: Jeffrey S. Lin, Andrew S. Pekosz, Andrew B. Feldman
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Patent number: 9579374Abstract: Certain embodiments of the present invention include a method for rapidly designing pharmaceutical preparations for preventing viral infection that have adapted to growth in various culturing systems but maintain antigenic similarity to the original virus. The method may include serial passaging a plurality of instances of a targeted virus strain from an infected first animal in respective droplets in second animal cells, in parallel synthesizing antibodies that neutralize the targeted virus strain in a third animal, and selecting the second animal cell-adapted viral lineages that are neutralized by the antibodies to identify second animal cell-adapted viral lineages having antigenic similarity to the targeted virus strain.Type: GrantFiled: December 3, 2014Date of Patent: February 28, 2017Assignee: The Johns Hopkins UniversityInventors: Jeffrey S. Lin, Andrew S. Pekosz, Andrew B. Feldman
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Publication number: 20160215282Abstract: A method for synthesizing an antiserum for rapid-turnaround therapies includes collecting antibody-secreting cells from a test subject, wherein the test subject has been exposed to a target biological agent and has produced an antibody response; selecting a subset of the antibody-secreting cells, the subset of the antibody-secreting cells producing antibodies that neutralize the target biological agent; generating variable-region-coding DNA sequences from the antibodies that neutralize the target biological agent; tagging amplicons of the variable-region-coding DNA sequences with unique nucleic acid identifiers to associate the variable-region-coding DNA sequences derived from individual ones of the subset of the antibody-secreting cells; analyzing antibody-type distribution in a natural immune response; synthesizing antibodies from the variable-region-coding DNA sequences to form synthetic antibodies; and mixing the synthetic antibodies in a proportion equal to the antibody-type distribution in the natural iType: ApplicationFiled: January 20, 2016Publication date: July 28, 2016Inventors: Jeffrey S. Lin, Andrew B. Feldman, Jared D. Evans, Joshua T. Wolfe, David Weitz, John Heyman, Andrew S. Pekosz
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Publication number: 20160175425Abstract: Certain embodiments of the present invention include a method for rapidly designing pharmaceutical preparations for preventing viral infection that have adapted to growth in various culturing systems but maintain antigenic similarity to the original virus. The method may include serial passaging a plurality of instances of a targeted virus strain from an infected first animal in respective droplets in second animal cells, in parallel synthesizing antibodies that neutralize the targeted virus strain in a third animal, and selecting the second animal cell-adapted viral lineages that are neutralized by the antibodies to identify second animal cell-adapted viral lineages having antigenic similarity to the targeted virus strain.Type: ApplicationFiled: December 3, 2014Publication date: June 23, 2016Inventors: Jeffrey S. Lin, Andrew S. Pekosz, Andrew B. Feldman
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Patent number: 9353396Abstract: The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.Type: GrantFiled: June 24, 2013Date of Patent: May 31, 2016Assignee: The Johns Hopkins UniversityInventors: Plamen A. Demirev, Nathan A. Hagan, Miquel D. Antoine, Jefffrey S. Lin, Andrew B. Feldman
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Publication number: 20150195189Abstract: Various exemplary embodiments relate to a method, network node, and non-transitory machine-readable storage medium including one or more of the following: receiving a message at the network node; selecting a routing tree of a plurality of routing trees based on a plurality of weights associated with the plurality of routing trees; determining a next hop network node for the message based on the selected routing tree; and forwarding the message to the next hop network node.Type: ApplicationFiled: January 7, 2014Publication date: July 9, 2015Applicant: Alcatel Lucent USA, Inc.Inventors: Peter J. Winzer, John E. Simsarian, Andrew B. Feldman, Thierry E. Klein
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Publication number: 20150079115Abstract: Food products and/or pharmaceutical preparations including (i) viral neutralizing antibodies or antibody fragments anchored to a probiotic microorganism and (ii) a carrier medium for delivering the viral neutralizing antibodies or antibody fragments anchored to probiotic microorganisms to the gut of a mammal. Also provided are methods of making food products and/or pharmaceutical preparations, which can be used to treat existing viral infections or prevent the spread or transmission of viral infection.Type: ApplicationFiled: September 16, 2014Publication date: March 19, 2015Inventors: Andrew B. Feldman, Jeffrey S. Lin, Kellogg Schwab, Timothy Julian, Christiane Wobus, David Weitz, David Heyman
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Publication number: 20150024378Abstract: Methods for analyzing DNA-containing samples are provided. The methods can comprise isolating a single genomic equivalent of DNA from the DNA-containing sample to provide a single isolated DNA molecule. The single isolated DNA molecule can be subjected to amplification conditions in the presence of one or more sets of unique molecularly tagged primers to provide one or more amplicons. Any spurious allelic sequences generated during the amplification process are tagged with an identical molecular tag. The methods can also include a step of determining the sequence of the one or more amplicons, in which the majority sequence for each code is selected as the sequence of the single original encapsulated target. The DNA-containing sample can be a forensic sample (e.g., mixed contributor sample), a fetal genetic screening sample, or a biological cell.Type: ApplicationFiled: July 15, 2014Publication date: January 22, 2015Inventors: Andrew B. Feldman, Jeffrey S. Lin, David Weitz, Assaf Rotem
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Publication number: 20130288356Abstract: The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.Type: ApplicationFiled: June 24, 2013Publication date: October 31, 2013Inventors: Plamen A. Demirev, Nathan A. Hagan, Miquel D. Antoine, Jefffrey S. Lin, Andrew B. Feldman
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Patent number: 8481281Abstract: The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.Type: GrantFiled: February 17, 2011Date of Patent: July 9, 2013Assignee: The Johns Hopkins UniversityInventors: Plamen A. Demirev, Nathan A. Hagan, Miquel D. Antoine, Jeffrey S. Lin, Andrew B. Feldman
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Publication number: 20110300552Abstract: The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.Type: ApplicationFiled: February 17, 2011Publication date: December 8, 2011Inventors: Plamen A. Demirev, Nathan A. Hagan, Miquel D. Antoine, Jeffrey S. Lin, Andrew B. Feldman
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Patent number: 7270948Abstract: Mass spectrometric techniques are provided for detecting the presence of parasites that accumulate unbound heme in red blood cells (including malaria parasites), based on the discovery that unbound heme can be detected and quantified using mass spectrometry. In one aspect of the invention, the method includes the steps of: obtaining a blood sample from the animal; preparing a test sample on a support from the blood sample, and inserting the support into a mass spectrometer for analysis. Next one obtains a mass spectrum of the test sample and determines whether the mass spectrum contains a mass/charge signature of unbound heme. If it is determined that the mass spectrum of the test sample shows the mass/charge signature of unbound heme, the animal is diagnosed as infected with malaria parasites.Type: GrantFiled: March 28, 2003Date of Patent: September 18, 2007Assignee: The Johns Hopkins UniversityInventors: Plamen A. Demirev, Andrew B. Feldman, Darin Kongkasuriyachai, Nirbhay Kumar, Peter F. Scholl, David J. Sullivan, Jr.
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Patent number: 7109038Abstract: Methods are described for detecting and quantifying occult blood in a biological sample using laser desorption mass spectrometry (LD MS). Biological samples that can be analyzed using various embodiments of the present invention include stool (fecal occult blood, FOB), and any bodily fluid including urine, cerebrospinal fluid and other bodily fluids. If the heme or heme metabolite is bound to protein, the sample is treated with acid before analysis to release the porphyrin. Some of the methods use LD MS with a time of flight analyzer (TOF) to detect and measure unbound heme, other hemoglobin metabolites and other molecules that have a porphyrin-based structure, e.g., bilirubin, biliverdin, protoporphyrin IX, and Zinc protoporphyrin in the biological sample. In other methods, matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is used to detect and quantify the individual ?- and ?-polypeptide chains of hemoglobin.Type: GrantFiled: June 6, 2003Date of Patent: September 19, 2006Assignee: The Johns Hopkins UniversityInventors: Peter F. Scholl, Plamen Demirev, Andrew B. Feldman
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Publication number: 20040241677Abstract: Techniques for automatically analyzing a biological sample with a microscope include obtaining a first digital image of a first field of view of the biological sample. Cell data and anomalous data are automatically determined. Cell data indicates an area co-located in the first digital image with a cell set of one or more cells of a particular type. Anomalous data indicates an area co-located in the first digital image with an anomalous set of zero or more particular objects that are anomalous to normal cells of the particular type. The cell data and the anomalous data are automatically combined to determine the particular objects inside the cell set in the first digital image. An analytical result for the biological sample is generated based on the particular objects inside the cell set. These techniques allow the automated classification and quantification of malaria in microscope views of blood smears, among other diseases.Type: ApplicationFiled: November 13, 2003Publication date: December 2, 2004Inventors: Jeffrey S Lin, Andrew B Feldman, Plamen A Demirev, Peter F Scholl, Sean P Murphy