Abstract: The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.

Abstract: The present invention provides methods for analyzing polynucleotides such as genomic DNA. In some embodiments, the disclosure provides a method for preparing and amplifying a genomic DNA library in situ in a fixed biological sample. The method comprises treating a fixed biological sample with an insertional enzyme complex to produce tagged fragments of genomic DNA. The method further comprises circularizing the tagged fragments of genomic DNA. The method further comprises amplifying the tagged fragments of genomic DNA.

Abstract: Systems and methods for personalizing dialogs are disclosed. A dialog system can provide a user interface to allow a first user to specify a second user to participate in an automated dialog, a specific response option, and a maximum time period for responding. The dialog system can send a message to the second user with the specific response option. The dialog system can branch the dialog based on the occurrence of an event or combination of events occurring in conjunction the second user. In a first branch, the dialog system is configured to send a second communication to the second user using a second communications channel, the second communication containing the specific response option. In a second branch, the dialog system is further configured to execute a third instruction associated with the dialog.

Abstract: The present invention provides methods for analyzing polynucleotides such as genomic DNA. In some embodiments, the disclosure provides a method for preparing and amplifying a genomic DNA library in situ in a fixed biological sample. The method comprises treating a fixed biological sample with an insertional enzyme complex to produce tagged fragments of genomic DNA. The method further comprises circularizing the tagged fragments of genomic DNA. The method further comprises amplifying the tagged fragments of genomic DNA.

Abstract: This invention relates to methods for controlling and monitoring access to network servers. In particular, the process described in the invention includes client-server sessions over the Internet. In this environment, when the user attempts to access an access-controlled file, the server subjects the request to a secondary server which determines whether the client has an authorization or valid account. Upon such verification, the user is provided with a session identification which allows the user to access to the requested file as well as any other files within the present protection domain.

Abstract: This invention relates to methods for controlling and monitoring access to network servers. In particular, the process described in the invention includes client-server sessions over the Internet. In this environment, when the user attempts to access an access-controlled file, the server subjects the request to a secondary server which determines whether the client has an authorization or valid account. Upon such verification, the user is provided with a session identification which allows the user to access to the requested file as well as any other files within the present protection domain.

Abstract: Systems and methods for personalizing dialogues are disclosed. A dialog system can provide a user interface to allow a first user to specify a second user to participate in an automated dialog, a specific response option, and a maximum time period for responding. The dialog system can send a message to the second user with the specific response option. The dialog system can branch the dialog based on the occurrence of an event or combination of events occurring in conjunction the second user. In a first branch, the dialog system is configured to send a second communication to the second user using a second communications channel, the second communication containing the specific response option. In a second branch, the dialog system is further configured to execute a third instruction associated with the dialog.

Abstract: Systems and methods for personalizing dialogs are disclosed. A dialog system can provide a user interface to allow a first user to specify a second user to participate in an automated dialog, a specific response option, and a maximum time period for responding. The dialog system can send a message to the second user with the specific response option. The dialog system can branch the dialog based on the occurrence of an event or combination of events occurring in conjunction the second user. In a first branch, the dialog system is configured to send a second communication to the second user using a second communications channel, the second communication containing the specific response option. In a second branch, the dialog system is further configured to execute a third instruction associated with the dialog.

Abstract: Systems and methods for personalizing marketing dialogues are disclosed. Specifically, embodiments of the systems and methods presented may associate a variable to each event in a script. A variable may be associated with each event. A first instruction in the script may be operable to send a first communication to a user from a server via the network. A second instruction may be operable to according to the script based on the stored set of user information and the variable associated with the first event.

Abstract: Systems and methods for personalizing dialogues are disclosed. A dialog system can provide a user interface to allow a first user to specify a second user to participate in an automated dialog, a specific response option, and a maximum time period for responding. The dialog system can send a message to the second user with the specific response option. The dialog system can branch the dialog based on the occurrence of an event or combination of events occurring in conjunction the second user. In a first branch, the dialog system is configured to send a second communication to the second user using a second communications channel, the second communication containing the specific response option. In a second branch, the dialog system is further configured to execute a third instruction associated with the dialog.

Abstract: An electronic commerce system includes a client computer and a server computer interconnected by a public packet switched communications network. The client computer is programmed to transmit to the server computer an order acceptance request terms or conditions of a proposed offer for a purchase, including multiple options of at least one of the terms or conditions of the offer. The server computer is programmed to process the order acceptance request based on pre-programmed criteria and based on the processing of the order acceptance request to transmit to the client computer an order acceptance response. The order acceptance response includes a plurality of amendments to the proposed offer for the purchase, including selection of an option of the at least one of the terms or conditions.

Abstract: Link traversals leading from an advertisement on the Web to a product page are determined. The determined link traversals are used as a basis for charging for advertising, for example, by counting the number of link traversals from the advertising page to a product page, or by counting the number of sales resulting from such traversals. The effectiveness of advertising on the Web is evaluated by measuring the number of sales or transactions resulting from link traversals from the advertisement to the page.

Abstract: Service requests from a client to a server system through a network are processed. A session identifier is appended to requests for information. The server responds to the requests from the client by returning the requested information to the client. Requests to particular information are counted, exclusive of repeated requests from a common client.

Abstract: An electronic commerce system includes a client computer and a server computer interconnected by a public packet switched communications network. The client computer is programmed to transmit to the server computer an order acceptance request that includes a plurality of terms or conditions of a proposed offer for a purchase, including multiple options of at least one of the terms or conditions of the offer. The server computer is programmed to process the order acceptance request based on pre-programmed criteria and, based on the processing of the order acceptance request, to transmit to the client computer an order acceptance response that includes a plurality of amendments to the proposed offer for the purchase, including selection of an option of the at least one of the terms or conditions.

Abstract: A system is provided that enables customers to search for event tickets for purchase. A computer interacts with the user to allow the user to specify criteria for event tickets the user wishes to purchase. The system presents relevant event ticket results, and provides filters for the user to further refine those search results. The user selects an event, and then selects tickets for that event. Ticket availability is shown, with seat locations indicated directly on an interactive venue map. For purchase, the user is directed to the selling ticket provider.

Abstract: Systems and methods for personalizing marketing dialogues are disclosed. Specifically, embodiments of the systems and methods presented may associate a variable to each event in a script. A variable may be associated with each event. A first instruction in the script may be operable to send a first communication to a user from a server via the network.

Abstract: Systems and methods for personalizing marketing dialogues are disclosed. Specifically, embodiments of the systems and methods presented may allow obtain a set of user actions from a participant. The set of user information may include user information, such as demographics, and may also contain event related information, such as a purchase or the user clicking on a link in an email. A program or script may be generated having decision branches. At each decision branch, a first dialogue or a second dialogue may be selected based on the user information. The personalized program or script may be presented to the user based on the user information.

Abstract: Systems and methods for carrying on marketing dialogues are disclosed. Specifically, embodiments of the systems and methods presented may allow marketing dialogues to be carried on with a set of participants by sending a communication to each of the participants. Another set of participant may be assembled from the initial participants and an action may then be taken with respect to this second set of participants.

Abstract: A system is provided that enables customers to search for event tickets for purchase. A computer interacts with the user to allow the user to specify criteria for event tickets the user wishes to purchase. The system presents relevant event ticket results, and provides filters for the user to further refine those search results. The user selects an event, and then selects tickets for that event. Ticket availability is shown, with seat locations indicated directly on an interactive venue map. For purchase, the user is directed to the selling ticket provider.

Abstract: Systems and methods for personalizing marketing dialogues are disclosed. Specifically, embodiments of the systems and methods presented may allow obtain a set of user actions from a participant. The set of user information may include user information, such as demographics, and may also contain event related information, such as a purchase or the user clicking on a link in an email. A program or script may be generated having decision branches. At each decision branch, a first dialogue or a second dialogue may be selected based on the user information. The personalized program or script may be presented to the user based on the user information.